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RNA INTERFERENCE 7
Custom-Made psiRNA
InvivoGen provides a complete siRNA service to help you speed-up your gene silencing experiments. Just give us the accession number of
your gene of interest and we will take care of all the steps, from helping you choose the target sequence on your gene to sequencing the
resulting siRNA insert. InvivoGen provides a choice of plasmidic vectors to better suit your needs.
➥ Full Custom Service
➥ Your siRNA vector Ready-to-Use
➥ Cost-effective
➥ Rapid Processing
Features and Benefits
Choose your siRNA sequence
You can either send us your siRNA sequence or, using the siRNA Wizard
software, InvivoGen will select the best candidate siRNA sequence for
your target gene.
Choose your psiRNA Plasmid
- psiRNA-h7SK (see page 144)
- psiRNA-DUO (see page 145)
Choose your selectable marker
psiRNA plasmids are available with various selectable markers that confer
antibiotic resistance in both E. coli and mammalian cells:
- Blasticidin - Zeocin
- Hygromycin - GFP::Zeo
- Kanamycin / G418
Description
Construction of custom-made psiRNA plasmids includes the following:
- Selection of the siRNA sequence for the target gene
- Synthesis of the siRNA insert oligonucleotides
- Cloning of the annealed oligonucleotides in psiRNA
- Sequencing of the siRNA insert
Contents and Storage
Custom-made psiRNA are provided as 20 µg of lyophilized DNA. They
can also be provided as a kit that contains in addition
- 20 µg of psiRNA-EGFP or psiRNA-LucGL3 as a control
- 1 vial of LyoComp GT116
- 4 pouches of the corresponding Fast-Media ®
Products are shipped at room temperature. Store at -20°C.
150 www.invivogen.com/custom-cloning
Although the siRNA Wizard software can facilitate the selection
of functional shRNAs, there is no guarantee that the resulting
shRNA will be effective enough for your experiment. Thus it is
usually recommended to select 3-5 candidate shRNAs.
Furthermore, most journal article reviewers require that gene
silencing results be confirmed with a second effective siRNA to a
target before an article is accepted for publication.
Orders of multiple psiRNA plasmids targeting the same gene will
be eligible for a volume discount.
PRODUCT QTY CAT. CODE
Custom-made psiRNA 20 μg p-custom
Custom-made psiRNA Kit 1 kit k-custom