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4 CYTOKINE SIGNALING TABLE of CONTENTS Cytokine Expression Cytokine and Related Genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 Cytokine Detection Anti-Cytokine Neutralizing IgA Antibodies . . . . . . . . . . . . . . . . . . . 104 Blue Cytokine Reporter Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 HEK-Blue Cytokine Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116 5 ANTIBODIES & VACCINATION Antibodies Antibody Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 Antibody Isotype Collections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 IgA Antibody Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 IgA Antibody Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123 IgA Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 IgG Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125 Vaccination Vaccine Adjuvants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126 OVA Antigens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129 DNA Vaccination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 6 CpG-FREE DNA CpG-Free Plasmids pCpGfree Plasmids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134 CpG-Free Genes Synthetic CpG-free Genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138 Custom-Made CpG-free Genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 7 RNA INTERFERENCE psiRNA System psiRNA Cloning Plasmids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142 psiTEST System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146 Interferon response: Off Target effect IFNr qRT-Primers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 psiRNA Vectors Ready-made psiRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Custom-made psiRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 8 INHIBITORS PRR Signaling Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 Cytokine Receptor Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Ion Channel Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Nuclear Export Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 NF-kB Activation Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 8 INHIBITORS MAPK Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Protein Kinase Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Epigenetic Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Heat Shock Protein Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158 DNA Synthesis Inhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 9 CUSTOM SERVICES TLR/NOD Ligand Screening . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161 Custom-Made CpG-free Genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161 Custom Cloning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162 Large Scale Plasmid DNA Production . . . . . . . . . . . . . . . . . . . . . . . . 163 PRODUCT INFORMATION. . . . . . . . . . . . . . . . . . . . . 165 INTERNATIONAL DISTRIBUTORS. . . . . . . . . . 176
1 CELL CULTURE & TRANSFECTION Mycoplasma Detection and Elimination 7 Reporter Detection Kits 12 Selective Antibiotics 16 Transfection Reagents 19 Induced Pluripotent Stem Cells 20
Page 2 and 3: INNOVATION WITHIN REACH InvivoGen
Page 4 and 5: CONTENTS AT A GLANCE 1 CELL CULTURE
Page 8 and 9: Mycoplasma Detection & Elimination
Page 10 and 11: PlasmoTest Contents - HEK-Blue -2
Page 12 and 13: Primocin - The Antimicrobial Shiel
Page 14 and 15: SEAP Reporter Gene System Secreted
Page 16 and 17: HEK-Blue Detection - Real-Time Det
Page 18 and 19: Blasticidin S Description Blasticid
Page 20 and 21: LyoVec New formulation LyoVec is
Page 22 and 23: Poly-Arginine-HA Tagged Reprogrammi
Page 24 and 25: LENTI-Smart - Lentiviral Vector Pr
Page 26 and 27: Lentiviral Vector Production and Ce
Page 28 and 29: Antibody generation using pFUSE-CHI
Page 30 and 31: Engineering Fc regions for altered
Page 32 and 33: pBROAD & pWHERE - Optimized Vector
Page 34 and 35: pSELECT-Tag - Expression of a GFP-,
Page 36 and 37: pVIVO - Expression of Two Genes of
Page 38 and 39: pORF Description pORF plasmids feat
Page 40 and 41: Prom A-List - Human and Rodent Pro
Page 42 and 43: PROMOTER GENE TISSUE DISTRIBUTION S
Page 44 and 45: ChemiComp E. coli ChemiComp E. coli
Page 46 and 47: Fast-Media ® Preparation 1. Empty
Page 48 and 49: RIG-I-like Receptors 54 RLR & RLR-R
Page 50 and 51: Toll-Like Receptors Toll-Like Recep
Page 52 and 53: www.invivogen.com/innate-immunity 5
Page 54 and 55: IPAF and NAIP5 IPAF (NLRC4, CLAN/CA
Page 56 and 57:
C-Type Lectin Receptors C-type lect
Page 58 and 59:
pUNO pUNO-HA Toll-Like Receptors (T
Page 60 and 61:
GENE NAME/ALIASES DESCRIPTION CAT.
Page 62 and 63:
pDUO - Gene Associations Features a
Page 64 and 65:
pZERO-TLR & pDeNy - Dominant Negati
Page 66 and 67:
293/TLR & 293/NOD Clones 293/TLR Cl
Page 68 and 69:
Response of HEK-Blue hTLR2 cells t
Page 70 and 71:
Ramos-Blue Cells - NF-kB/AP-1 Repo
Page 72 and 73:
MEF Cells C3H/TLR4mut & C3H/WT MEFs
Page 74 and 75:
Antibodies for Detection ANTIBODY D
Page 76 and 77:
- NF-kB binding site: Nuclear facto
Page 78 and 79:
PRODUCT ORIGIN/DESCRIPTION TLR2 Ago
Page 80 and 81:
PRODUCT ORIGIN/DESCRIPTION TLR9 Ago
Page 82 and 83:
PRODUCT ORIGIN/DESCRIPTION Dectin-1
Page 84 and 85:
LPS-PG (Porphyromonas gingivalis) R
Page 86 and 87:
• FLA-ST ultrapure was purified b
Page 88 and 89:
1. Zhao H. et al., 2004. Contributi
Page 90 and 91:
1. Hornung V. et al., 2006. 5'-Trip
Page 92 and 93:
TLR/NOD Ligand Screening InvivoGen
Page 94 and 95:
Signal Transduction Inhibitors Invi
Page 96 and 97:
Pepinh-MYD - MyD88 inhibitory pepti
Page 98 and 99:
1. Martinon F,. et al., 2002. The i
Page 100 and 101:
Nigericin - NLRP3 Inflammasome Indu
Page 102 and 103:
Autophagy is a pathway by which cyt
Page 104 and 105:
4 CYTOKINE SIGNALING Cytokine Genes
Page 106 and 107:
Blue Cytokine Reporter Cells Blue
Page 108 and 109:
HEK-Blue IFN-a/b Cells - Human Typ
Page 110 and 111:
HEK-Blue IL-6 Cells - IL6 Reporter
Page 112 and 113:
HEK-Blue IL-1b Cells - IL-1b Repor
Page 114 and 115:
HEK-Blue IL-33/IL-1b Cells - IL-33
Page 116 and 117:
HEK-Blue CD40L Cells - CD40L Repor
Page 118 and 119:
5 ANTIBODIES & VACCINATION Antibodi
Page 120 and 121:
Antibody generation using pFUSE-CHI
Page 122 and 123:
Immunoglobulin A The mucosal surfac
Page 124 and 125:
IgA Detection and Quantification In
Page 126 and 127:
IgG Antibodies InvivoGen offers a s
Page 128 and 129:
Mediators? 1. Leroux-Roels G., 2010
Page 130 and 131:
Poly(I:C) Synthetic double-stranded
Page 132 and 133:
pVAC - Induction of Neutralizing An
Page 134 and 135:
CpG-Free DNA CpGs and the Immune Re
Page 136 and 137:
pCpGfree-basic & pCpGfree-promoter
Page 138 and 139:
pCpGfree-siRNA & pCpGfree-siRNADUO
Page 140 and 141:
Custom-Made CpG-Free Genes InvivoGe
Page 143 and 144:
RNA INTERFERENCE 7 psiRNA System I
Page 145 and 146:
RNA INTERFERENCE 7 psiRNA-h7SK - 7S
Page 147 and 148:
RNA INTERFERENCE 7 psiTEST System
Page 149 and 150:
RNA INTERFERENCE 7 Ready-Made psiRN
Page 151 and 152:
RNA INTERFERENCE 7 Custom-Made psiR
Page 153 and 154:
INHIBITORS 8 Inhibitors PRODUCT DES
Page 155 and 156:
INHIBITORS 8 Pattern Recognition Re
Page 157 and 158:
INHIBITORS 8 Triptolide - NF-kB Inh
Page 159 and 160:
INHIBITORS 8 Heat Shock Protein Inh
Page 161 and 162:
9 CUSTOM SERVICES TLR/NOD Ligand Sc
Page 163 and 164:
CUSTOM SERVICES 9 Custom Cloning :
Page 165 and 166:
CUSTOM SERVICES 9 Prices 164 TERMS
Page 167 and 168:
166 PRODUCT (QUANTITY) CAT. CODE PA
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
A 17-AAG 17-AAGH2 17-AEP-GA 2-Amino
Page 175 and 176:
- ODN 4084-F - ODN INH-1 - ODN INH-
Page 177 and 178:
ARGENTINA Genbiotech S.R.L. Tel: +5
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