Annual Progress Report on Malting Barley Research March, 2002

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102 Epstein, E. 1999. Silicon. Ann. Rev. Plant Mol. Biol. 50:641-664. Wan, Y., and P.G. Lemaux. 1994. Generation of large numbers of independently transformed fertile barley plants. Plant Physiol. 104:37-48. Zhang, S., M-J. Cho, T. Koprek, R. Yun, P. Bregitzer, and P.G. Lemaux. 1999. Genetic transformation of commercial germplasm of oat (Avena sativa L.) and barley (Hordeum vulgare L.) using in vitro shoot meristematic cultures derived from germinated seedlings. Plant Cell Rep. 18:959-966. Table 1. Comparative genotype regeneration responses to different concentrations of silicon in tissue culture media. All control means were set at one. Data are reported as number of green plants per petri dish as compared to the control response. Si Concentration (:M) Genotype Location 0 10 100 500 1000 5000 GP combined 1.0 1.9 1.1 2.5 1.0 0.7 Morex combined 1.0 0.8 1.4 1.5 1.2 1.3 Harrington Fargo 1.0 0.9 2.0 2.2 1.5 2.0 GP + Morex combined 1.0 1.5 1.2 2.2 1.0 0.9
103 Table 2. Agronomic performance of non-tissue cultured controls and plants regenerated from original and improved media. Parental line Media Heading date (julian) Height (cm) Lodging (%) Yield (bu/ac) Test weight (#/bu) % Plump Kernels 90Ab321 Improved 167 56.1 24.4 86.9 53.2 86.1 90Ab321 Original 165 59.0 9.7 89.1 53.5 88.9 90Ab321 control 165 58.0 14.4 90.7 53.1 87.8 Baronesse Improved 174 65.8 34.7 78.7 51.8 77.6 Baronesse Original 174 65.6 34.1 87.0 52.3 82.5 Baronesse control 173 63.4 31.3 88.0 52.4 83.1 Colter Improved 168 69.3 17.2 108.9 51.3 67.8 Colter Original 168 65.3 16.9 101.7 51.0 69.9 Colter control 167 67.4 5.6 110.7 52.2 78.0 Conlon Improved 168 68.8 6.9 99.9 53.9 96.2 Conlon Original 168 66.6 3.4 98.8 54.0 97.6 Conlon control 170 70.3 4.2 103.2 54.8 98.0 Crystal Improved 175 69.9 25.6 101.2 53.4 81.2 Crystal Original 175 70.6 16.3 100.9 53.9 83.2 Crystal control 173 72.3 29.4 101.9 53.4 78.3 Golden Promise Improved 175 57.5 30.0 78.0 51.2 60.9 Golden Promise Original 175 58.1 37.2 77.7 50.6 52.9 Golden Promise control 175 54.6 32.5 73.5 50.9 55.3 Morex Improved 169 70.5 26.6 84.8 50.1 69.5 Morex Original 169 72.9 30.6 79.7 49.6 71.3 Morex control 168 72.5 9.4 92.6 51.2 82.9 ND15477 Improved 169 70.3 6.3 98.7 52.0 81.7 ND15477 Original 170 71.6 3.1 100.0 52.6 82.0 ND15477 control 169 71.3 7.5 107.6 52.5 86.7 mean of all lines Improved 171 66.0 21.4 92.0 52.1 77.7 mean of all lines Original 170 66.2 18.9 91.8 52.2 78.4 mean of all lines control 170 65.9 17.2 95.6 52.4 80.1
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
Page 59 and 60: 51 BARLEY TRANSFORMATION G.J. Muehl
Page 61 and 62: 53 We have available the sugarcane
Page 63 and 64: 55 Secondly, we tried to grow F. gr
Page 65 and 66: 57 References: Issac, S. and Jennin
Page 67 and 68: 59 Huntley-dryland) with two replic
Page 69 and 70: 61 Table 2. 1992 thru 2001 Overall
Page 71 and 72: 63 performance for Montana farmers.
Page 73 and 74: 65 Blake, T,. Hensleigh P., Boss D.
Page 75 and 76: 67 settling tower cannot accommodat
Page 77 and 78: Table 3. Seedling reaction to strip
Page 79 and 80: 71 TWO-ROWED BARLEY IMPROVEMENT PRO
Page 81 and 82: Table 1. Agronomic trait comparison
Page 83 and 84: 75 mutants reduce plant vigor and g
Page 85 and 86: 77 SIX-ROWED BARLEY IMPROVEMENT PRO
Page 87 and 88: 79 this variety has decreased over
Page 89 and 90: Table 7. Malt quality comparisons o
Page 91 and 92: 83 • Management studies for malt
Page 93 and 94: 85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109: 101 trials of plants regenerated fr
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
Page 133 and 134: 125
Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143 and 144: 135 D.M. Wesenberg - spring and win
Page 145 and 146: 137 A STUDY OF THE MALTING QUALITY
Page 147 and 148: 139 Methods. Our malting schedule.
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
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153 Sissons MJ and MacGregor AW (19
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µM Carbohydrate / Unit of α-Gluco
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157 Tissue-specific gene products f
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159 The promoters were subcloned fr
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161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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