Annual Progress Report on Malting Barley Research March, 2002

More documents

Recommendations

Info

86 3. Screen breeders lines for resistance to net blotch. 400 breeder’s lines were screened for net blotch (Pyrenophora teres f. teres) resistance in the greenhouse last year. Ninety eight out of 219 tested 2-rowed lines were found to have good to high levels of resistance (with infection types 4. 3, 2, or lower). Among the 6rowed lines, half of them only exhibited a moderate level of resistance to net blotch, only lines ND19711, ND 19742, and ND19289 exhibited low infection phenotypes to isolate 89-19 of P. teres f. teres. These results confirmed previous screening which clearly showed that in lines originating from the the breeding programs at NDSU, 2 row barley has greater resistance than 6 row. 4. Screen barley for resistance to crown rust. A further study was conducted for the genetics of resistance to barley crown rust (Puccinia coronata var. hordei) in wheat.. F2 populations, derived from Chris monosomic wheat lines crossed to a spring type susceptible selection of PI350005, were inoculated with crown rust isolate 91-36sp1. The disease reactions of the F2 progenies fit closely to the expected single gene ratio and the phenotype data was provided for the on-going molecular mapping of the resistance gene in wheat. Several barley lines were evaluated for their resistance to crown rust in the field. Logan, PC249a, and Hor2596 were found resistant to crown rust with reaction types of MR or R. Aim and Azure are susceptible to crown rust in the field. 5. Molecular mapping of the barley stem rust resistance gene rpg4. In a collaborative effort on physical mapping of the barley stem rust resistance gene rpg4 , barley pathology project of NDSU has screened 100 OS6 x Harrington F2 families and SQ501 derivatives to pathotype QCC-2 of Puccinia graminis f. sp. tritici at both low and high temperatures. In addition, 102 recombinant barley families for rpg4 resistance were also tested against pathotype QCC-2. Analysis is ongoing. 6. Verifying the existence of new spot blotch races. With Dr J Frankowiak we have screened 46 diverse genetic barely lines in the glasshouse against 3 putative new races of Cochliobolus sativus that caused much higher levels of disease in the field in 2001. Preliminary results indicate that these are new races that are able to cause increased disease. OTHER FUNDS AND FUTURE DIRECTIONS As one of the principal investigators on the SBARE Malting Barley for Western North Dakota project I have developed collaborations with Dr Marcia McMullen and Mr Roger Ashley to substantially increase the numbers of barley fields sampled for leaf, stem head and root diseases in Western North Dakota. Currently about 190 barley fields are sampled annually with patchy distributions in some parts of the state. We aim to increase the sampling to 500 fields. This sampling will not only provide spatial data that will be available to farmers and researchers about actual problems in the field but will provide the project with a bank of isolates that can be used to monitor pathotype distributions. The money allocated from SBARE does not fully cover the anticipated project expenditure.
87 With funding from the US Wheat and Barley Scab Initiative, obtained by Dr L Dahleen USDA-ARS Fargo, we are screening in the glasshouse 60 lines of transgenic Conlon barley with anti toxin gene TRI101 and yeast metabolite transporter gene PDR5 for resistance to Fusarium head blight. Preliminary results indicate that lines containing either gene can be effective at reducing disease. Promising lines will be tested in the field in 2002. Another future direction will be to look at manipulating inoculum levels in the stubble of previous crops to reduce the disease pressure on barley. To date most disease control methods are aimed at reducing disease levels once the infection has begun. Manipulation of stubble to reduce inoculum will take place by a combination of mechanical, chemical and microbiological methods. Collaborative research projects are under negotiation with Dr D Schisler USDA-ARS Peoria Il to test biological control strains of Cryptococcus nodaensis yeast and with Dr J. Kloepper Auburn University, AL to test a three component Bacillus mix for reduction of inoculum potential and disease due to Fusarium graminearum. With Dr P Bregitzer USDA-ARS Aberdeen ID and Peggy Lemaux UC Berkley, we have negotiated a collaborative project in which we test transgenic Drummond barley with antifungal genes TLP1 and TLP4 and trichothecene pathway genes TRI101 and TRI102 against a range of barley diseases. Germplasm is not expected to become available for this project for 12 months at which time funding will be sought to support this component of the project. With Dr B Steffenson UM and Dr D Garvin USDA-ARS Minneapolis I have negotiated a collaborative project to screen 120 H. vulgare ssp spontaneum introgression lines for resistance to disease caused by a range barley pathogens. Personnel: Stephen Neate, Associate Professor / Project Leader (since October 8 th 2001) Glen Statler, Prof / Dept Chair / Project Leader (until October 8 th 2001) Yongliang Sun, Research Specialist I Cooperators: Mr Roger Ashley, Dickinson Research Extension Center, ND Dr. Lynn Dahleen, USDA-ARS, Fargo, ND Dr. Jerome Franckowiak, NDSU, Fargo, ND Dr. Richard Horsley, NDSU, Fargo, ND Dr. Shahryar Kianian, NDSU, Fargo, ND Dr. Andy Kleinhofs, WSU, Pullman, WA Dr Joseph Kloepper, Auburn University, AL Dr Marcia McMullen, NDSU, Fargo, ND Dr David Schisler, USDA-ARS Peoria Il
Page 1 and 2:
Annual Pro
Page 3:
-i- INTRODUCTION The March 2002 <st
Page 6 and 7:
Barley Transformation G.J. Muehlbau
Page 9 and 10:
1 2000/2001 WINTER NURSERY: BARLEY
Page 11 and 12:
3 2001 Barley Stripe Rust Screening
Page 13 and 14:
5 Triumph/Tyra//Arupo, a selection
Page 15 and 16:
7 Washington (Fossum Cereals and Wa
Page 17 and 18:
9 This project is supported by the
Page 19 and 20:
11 ‘Triumph’, ‘Valier’, ‘
Page 21 and 22:
13 Table 2. Agronomic data for sele
Page 23 and 24:
15 Table 4. Agronomic data for sele
Page 25 and 26:
17 Table 8. Malting quality data* f
Page 27 and 28:
19 Six-rowed barley selections of c
Page 29 and 30:
21 Table 14. Agronomic data for sel
Page 31 and 32:
23 Table 16. Agronomic data for win
Page 33 and 34:
25 Table 19. Malting quality data*
Page 35 and 36:
Garnet/98Ab11865 Garnet/98Ab12895 9
Page 37 and 38:
29 rust resistant NSGC accessions o
Page 39 and 40:
31 DEVELOPMENT OF SIX-ROWED MALTING
Page 41 and 42:
33 backcrossing. The selfed lines w
Page 43 and 44: 35 MINNESOTA BARLEY IMPROVEMENT PRO
Page 45 and 46: 37 ADVANCED LINE EVALUATION Varieti
Page 47 and 48: 39 OTHER BARLEY DISEASES In 2001, r
Page 49 and 50: 41 Wingbermuehle, W. J., K.M. Belin
Page 51 and 52: 43 In 2001 winter/spring, over 300
Page 53 and 54: 45 assessments were made by countin
Page 55 and 56: 47 IDENTIFICATION OF NOVEL DISEASE
Page 57 and 58: 49 Unfortunately, disease levels in
Page 59 and 60: 51 BARLEY TRANSFORMATION G.J. Muehl
Page 61 and 62: 53 We have available the sugarcane
Page 63 and 64: 55 Secondly, we tried to grow F. gr
Page 65 and 66: 57 References: Issac, S. and Jennin
Page 67 and 68: 59 Huntley-dryland) with two replic
Page 69 and 70: 61 Table 2. 1992 thru 2001 Overall
Page 71 and 72: 63 performance for Montana farmers.
Page 73 and 74: 65 Blake, T,. Hensleigh P., Boss D.
Page 75 and 76: 67 settling tower cannot accommodat
Page 77 and 78: Table 3. Seedling reaction to strip
Page 79 and 80: 71 TWO-ROWED BARLEY IMPROVEMENT PRO
Page 81 and 82: Table 1. Agronomic trait comparison
Page 83 and 84: 75 mutants reduce plant vigor and g
Page 85 and 86: 77 SIX-ROWED BARLEY IMPROVEMENT PRO
Page 87 and 88: 79 this variety has decreased over
Page 89 and 90: Table 7. Malt quality comparisons o
Page 91 and 92: 83 • Management studies for malt
Page 93: 85 STUDIES ON BARLEY DISEASES AND T
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
Page 133 and 134: 125
Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143 and 144: 135 D.M. Wesenberg - spring and win
Page 145 and 146:
137 A STUDY OF THE MALTING QUALITY
Page 147 and 148:
139 Methods. Our malting schedule.
Page 149 and 150:
141 modification measures indicated
Page 151 and 152:
143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154:
145 Purification of a serine endope
Page 155 and 156:
147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158:
149 with the proteinase T will be s
Page 159 and 160:
151 10°C increase in thermostabili
Page 161 and 162:
153 Sissons MJ and MacGregor AW (19
Page 163 and 164:
µM Carbohydrate / Unit of α-Gluco
Page 165 and 166:
157 Tissue-specific gene products f
Page 167 and 168:
159 The promoters were subcloned fr
Page 169:
161 Fig. 3. Expression of Trx-Hth f
show all

Annual Progress Report on Malting Barley Research March, 2002

Create successful ePaper yourself

Delete template?

Save as template?