Annual Progress Report on Malting Barley Research March, 2002

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134 6. Breeding for Pest Resistance Increased efforts are underway to breed for resistance to relatively new or potential pest problems in the Pacific Northwest. The priority order of effort is barley stripe rust (collaborating with X. Chen and D. Wesenberg), soil borne root diseases (collaborating with R.J.Cook), Hessian fly (collaborating with R. Ratcliffe), and Russian wheat aphid (collaborating with D. Mornhinweg). Breeding lines resistant to these pests are emerging in the breeding program. Future Directions Future directions of the barley improvement program at WSU include the development of improved germplasm both at the pre- and post-cultivar release stages. Barley malting, brewing, and nutritional quality will continue to be emphasized along with yield and adaptation. New selection procedures will be employed to improve the efficiency, effectiveness, and acceleration of the cultivar development program. The barley genome mapping project will contribute to breeding efficiency and effectiveness through improved genetic knowledge of economically important traits and molecular markerassisted selection procedures. This is a particular emphasis currently. A gradual movement toward transformation of barley as techniques become available is ongoing with cooperative studies. Associated basic genetic and biochemical characterization studies of malting and nutritional quality traits will continue. WSU Barley Program Personnel S. E. Ullrich, Professor and Scientist, Project Leader. A. Kleinhofs, Professor/Scientist, Collaborator, molecular genetics and breeding, WSU. D. von Wettstein, R.A. Nilan Distinguished Barley Professor, Collaborator, proanthocyanidin free barley, transformation, WSU. V. A. Jitkov, Breeding Field Research Associate, in charge of field/greenhouse/seedhouse operations. J. A. Clancy, Research Technologist III, in charge of the barley quality and molecular genetics lab with partial support from AMBA. M.C. Dugger, Research Technologist I, breeding field/greenhouse/seedhouse assistant . C. Kruger, M.S. Graduate Research Assistant working on evaluation of barley traits associated with direct-drilled cropping systems. W. Hotchkin, M.S. Graduate Research Assistant. ADDITIONAL COOPERATIVE EFFORTS OCCUR WITH THE FOLLOWING SCIENTISTS: X. Chen - barley stripe rust, USDA-ARS, WSU. R.J. Cook - root diseases, WSU. B.-K. Baik – barley food quality, WSU.
135 D.M. Wesenberg – spring and winter regional nurseries, barley stripe rust, QTL validation, germplasm exchange, USDA-ARS, Aberdeen, ID. P.M. Hayes - QTL validation, germplasm exchange, OSU, Corvallis, OR. B.L. Jones - malting quality analyses, USDA-ARS, Madison,WI. D.W. Mornhinweg - Russian wheat aphid, USDA-ARS, Stillwater, OK. R.H. Ratcliffe – Hessian fly, USDA-ARS, West Lafayette, IN. I. Romagosa- genetic studies of barley adaptation and quality and breeding hulless barley, University of Lleida, Lleida, Spain. G. Arias- breeding and malting quality, EMBRAPA, Passo Fundo, R.S., Brazil. SELECTED 2001 PUBLICATIONS: Horvath, H., L.G. Jensen, O.T. Wong, E. Kohl, S.E. Ullrich, J. Cochran, C.G. Kannangara, and D. von Wettstein. 2001. Stability of transgene expression, field performance and recombination breeding of transformed barley lines. Theor. Appl. Genet. 102:1-11. Wesenberg, D.M., D.E. Burrup, W.M. Brown, Jr., V.R. Velasco, J.P. Hill, J.C. Whitmore, R.S. Karow, P.M. Hayes, S.E. Ullrich, and C.T. Liu. 2001. Registration of ‘Bancroft’ barley. Crop Sci. 41:265-266. Marquez-Cedillo, L.A., P.M. Hayes, A. Kleinhofs, W.G. Legge, B.G. Rossnagel, K. Sato, S.E. Ullrich, D.M. Wesenberg, and the NABGMP. 2001. QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups. Theor. Appl. Genet. 103:625-637. Ullrich, S.E., C.E. Muir, and J.A. Froseth. 2001. Registration of ‘Bear’ barley. Crop Sci. 41:1994-1995. Ullrich, S.E., C.E. Muir, M.L. Nelson, and J.A. Froseth. 2001. Registration of ‘Washford’ barley. Crop Sci. 41:1995. Ullrich, S.E., J.W. Burns, P.E. Reisenauer, V.A. Jitkov, D. von Wettstein, and A. Kleinhofs. 2001. Spring barley performance in 2000. Wheat Life 44(1):46-48. Ullrich, S.E. and M. Palmer Sullivan. 2001. WSU releases Farmington, a new two-row spring barley variety. Wheat Life 44(5):20. Dofing, S.M., S.S. Jones, S.E. Ullrich, K.K. Kidwell, K.G. Campbell, and D. Boze. 2001. 2000 cereal variety evaluation results. Dept. of Crop and Soil Sci. Tech. <strong>Report</strong> 01-1. WSU. 120 pp. Ullrich, S. E., V.A. Jitkov, J. A. Clancy, M.C. Dugger, and C.I. Kruger. 2001. Barley improvement research. pp. 33-36. In J. Burns and R. Veseth (ed.). 2001 field day proceedings: Highlights of research progress. Dept. of Crop and Soil Sci. Tech <strong>Report</strong> 01-4. WSU. Cook, R.J., S.E. Ullrich, W.F. Schillinger. 2001. Performance of advanced lines and varieties of spring barley seeded directly into wheat or barley stubble. pp. 87-88. In J.
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
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51 BARLEY TRANSFORMATION G.J. Muehl
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53 We have available the sugarcane
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55 Secondly, we tried to grow F. gr
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57 References: Issac, S. and Jennin
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59 Huntley-dryland) with two replic
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61 Table 2. 1992 thru 2001 Overall
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63 performance for Montana farmers.
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65 Blake, T,. Hensleigh P., Boss D.
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67 settling tower cannot accommodat
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Table 3. Seedling reaction to strip
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71 TWO-ROWED BARLEY IMPROVEMENT PRO
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Table 1. Agronomic trait comparison
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75 mutants reduce plant vigor and g
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77 SIX-ROWED BARLEY IMPROVEMENT PRO
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79 this variety has decreased over
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Table 7. Malt quality comparisons o
Page 91 and 92: 83 • Management studies for malt
Page 93 and 94: 85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
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Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141: 133 2. Direct Seeding Cropping Syst
Page 145 and 146: 137 A STUDY OF THE MALTING QUALITY
Page 147 and 148: 139 Methods. Our malting schedule.
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
Page 161 and 162: 153 Sissons MJ and MacGregor AW (19
Page 163 and 164: µM Carbohydrate / Unit of α-Gluco
Page 165 and 166: 157 Tissue-specific gene products f
Page 167 and 168: 159 The promoters were subcloned fr
Page 169: 161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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