Annual Progress Report on Malting Barley Research March, 2002

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138 Barley samples malted and subjected to malting quality analysis. 2000 Crop Year Summary 4,674 samples were received for analysis: Breeders’ line micro-malts (170 g) 2,673 Breeders’ line micro-malts (120 g) 340 Western Spring Nursery 102 Mississippi Valley Nursery 130 AMBA Pilot malting samples 22 Breeders’ experimental studies 993 ‘In House’ experimental studies 414 Total 4,674 2001 Crop Year Summary 3,523 samples have been received (as of 2-20-02) for analysis: Breeders’ line micro-malts (170 g) 2,611 Breeders’ line micro-malts (120 g) 142 Western Spring Nursery 100 Mississippi Valley Nursery 116 AMBA Pilot malting samples 22 Malts for experimental studies 382 Malts for ‘in house’ experiments 150 Total 3,523 New equipment. During the year, we replaced our LECO 428 Nitrogen Analyzer with an updated LECO 528 Analyzer. The analytical principles used by the instruments are identical and the results from the two are interchangeable. We also purchased and have received three new steep tanks this year. These will be installed and tested as soon as the current crop year samples have all been analyzed. Studies with the Joe White Malting Systems Christopher Martens, Allen Budde and Berne Jones Cereal Crops Research Unit, USDA, ARS Objectives. Our Joe White (JW) Micromalting systems have been optimized to produce malts that have quality parameters that are similar to those from malts prepared with our traditional malting system and comparable to commercial malts. The use of the JW micromalters has increased our annual malting capacity by 1,500 samples. During this year, we examined how changes in some steps of the malting process affected the malt quality. In addition, we started using the JW machines to carry out the regular micromalting of ‘experimental’ barleys from collaborating barley researchers.
139 Methods. Our malting schedule. We use the following protocol with the JW Micromalting systems: Steep: Air rest airflow rate of 30%, with 0% recirculation. All stages at 19ºC. 7 hours (h) wet, 8h air, 4h wet, 5h air, 2h wet, 2h air, and 2h wet (30h total). Germination: Airflow rate of 30%, with 0% recirculation. 4 turns every 2h with a 30º trim angle. 48h 18ºC, 24h 17ºC, and 24h 16ºC. Kiln: All stages 40% airflow. 0% recirculation for stages 1-3, 50% for stage 4, and 75% for stage 5. 10h 49ºC, 4h 54ºC, 3h 60ºC, 2h 68ºC, and 3h 85ºC, with a 30 min. ramp between stages. Results. High soluble protein levels. This schedule produced malt that was consistent with that from our traditional system. However, the results of the 2001 AMBA Collaborative study revealed that the malts produced in both our JW and traditional systems had elevated wort soluble protein levels, compared to those from the other collaborators. We are, therefore, still conducting research that is aimed at reducing the soluble protein levels in our micromalts. The Effect of air recirculation during germination. Researchers at the JW Maltings had indicated that using less recirculated air during the steep air rests and germination would decrease the grain modification rate. We have integrated this concept into our malting schedule, but we needed to confirm this effect experimentally. Multiple samples of each of four malting barley varieties (Harrington, Morex, Conlon, and Robust) were steeped in one of the JW micromalting machines. Half of the samples were then germinated in the same machine with 100% air recirculation, while the other half were transferred to a second JW instrument and germinated with 0% recirculated air. All of the samples were kilned with our normal 24-hour method. The quality parameters of the finished malts are compared in Table 1.
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
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51 BARLEY TRANSFORMATION G.J. Muehl
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53 We have available the sugarcane
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55 Secondly, we tried to grow F. gr
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57 References: Issac, S. and Jennin
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59 Huntley-dryland) with two replic
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61 Table 2. 1992 thru 2001 Overall
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63 performance for Montana farmers.
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65 Blake, T,. Hensleigh P., Boss D.
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67 settling tower cannot accommodat
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Table 3. Seedling reaction to strip
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71 TWO-ROWED BARLEY IMPROVEMENT PRO
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Table 1. Agronomic trait comparison
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75 mutants reduce plant vigor and g
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77 SIX-ROWED BARLEY IMPROVEMENT PRO
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79 this variety has decreased over
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Table 7. Malt quality comparisons o
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83 • Management studies for malt
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85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
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Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143 and 144: 135 D.M. Wesenberg - spring and win
Page 145: 137 A STUDY OF THE MALTING QUALITY
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
Page 161 and 162: 153 Sissons MJ and MacGregor AW (19
Page 163 and 164: µM Carbohydrate / Unit of α-Gluco
Page 165 and 166: 157 Tissue-specific gene products f
Page 167 and 168: 159 The promoters were subcloned fr
Page 169: 161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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