Annual Progress Report on Malting Barley Research March, 2002

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Stem Roots Leaves Flag leaf Developing Spike Coleoptile (3d) First Leaf (3 d) 160 Anthers Ovary Epicarp Pericarp/Testa Endosperm Embryo Rachis Lemma/Palea Fig. 1. EpiLTP gene expression. Left: Barley EpiLTP gene expression in various vegetative tissues EpiLTP is highly expressed in the developing spike but not in leaf, stem or root tissues. Center: Barley EpiLTP gene expression in 3-day old seedlings. EpiLTP is highly expressed in the coleoptile tissue. Right: Barley EpiLTP gene expression in various spike tissues. EpiLTP is highly expressed in the epicarp and embryo, but not in endosperm, anther, rachis or awn tissues. Low expression was found in the ovary. Fig. 2. Barley EpiLTP gene structure. Homology search of putative cis-acting DNA elements was performed using PLACE database (Higo et al., 1999). The positions of different putative cis-acting DNA elements are represented by triangles. Name, code and potential response or tissue-specificity of each of these DNA elements is summarized in table (right). Awns
161 Fig. 3. Expression of Trx-Hth fusion protein genes in E. coli. A: Separation of bacterial proteins by SDS- PAGE. Soluble protein from induced cultures Lane 1, standards. Proteins from bacteria harboring Trx (wedge) only (lane 2), Trx-Hth1 (lane 3), and Trx-Hth2 (lane 4; wedge marks Trx-HTH fusion protein). B. Top: Northern blot using RNA extracted from transformed E. coli and probed with 32 P-labeled Hth1 cDNA. RNA from the uninduced (lane 1) and IPTG-induced (Lane 2) bacteria harboring Trx-Hth1 and uninduced (lane 3) and induced (lane4) bacteria harboring Trx-Hth2. B: Bottom: The stripped northern blot was rehybridized with 32 P-labeled cDNA probes synthesized from bacterial total RNA. A B B 16S (1.5kb ) 80S Trx-HTH1 Trx-HTH2 IPTG - + - + 1 2 3 4 8-mer 14-mer 16S Fig. 4. Analysis of Hth2 mRNA on polyribosomes. A: Leaf polyribosomes from transformant B03 were separated on a sucrose density gradient. 8-mer refers to mRNA with 8 ribosomes attached. B: Equal volume fractions were collected, and RNA was applied to a Nytran filter through a slot blot apparatus. The blot was probed with 32 P-dCTP labeled Hth1 cDNA. C: RNAs from free and membrane-bound (mem) polyribosomes from leaves of lines B01 and B03 were northern blotted and probed as above. Control lane (con) is total leaf RNA from line B03. D: Blot for panel C was stripped and reprobed with labeled total RNA cDNA, as above. Dark band is 18S rRNA; light upper band is 28S rRNA. C D A kDa 1 2 3 4 9 4 6 7 4 3 3 Trx 0 2 0 B03 B01 free mem free mem con
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
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51 BARLEY TRANSFORMATION G.J. Muehl
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53 We have available the sugarcane
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55 Secondly, we tried to grow F. gr
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57 References: Issac, S. and Jennin
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59 Huntley-dryland) with two replic
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61 Table 2. 1992 thru 2001 Overall
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63 performance for Montana farmers.
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65 Blake, T,. Hensleigh P., Boss D.
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67 settling tower cannot accommodat
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Table 3. Seedling reaction to strip
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71 TWO-ROWED BARLEY IMPROVEMENT PRO
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Table 1. Agronomic trait comparison
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75 mutants reduce plant vigor and g
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77 SIX-ROWED BARLEY IMPROVEMENT PRO
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79 this variety has decreased over
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Table 7. Malt quality comparisons o
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83 • Management studies for malt
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85 STUDIES ON BARLEY DISEASES AND T
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87 With funding from the US Wheat a
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89 MALTING AND BREWING QUALITY OF B
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91 β-glucans, it might be most ins
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10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
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95 Bolin, P., Schwarz, P., Jones, B
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97 Results Results from field and g
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99 DEVELOPING BARLEY TISSUE CULTURE
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101 trials of plants regenerated fr
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103 Table 2. Agronomic performance
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105 BSMV spread between entries. Ho
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107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
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Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
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Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143 and 144: 135 D.M. Wesenberg - spring and win
Page 145 and 146: 137 A STUDY OF THE MALTING QUALITY
Page 147 and 148: 139 Methods. Our malting schedule.
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
Page 161 and 162: 153 Sissons MJ and MacGregor AW (19
Page 163 and 164: µM Carbohydrate / Unit of α-Gluco
Page 165 and 166: 157 Tissue-specific gene products f
Page 167: 159 The promoters were subcloned fr
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Annual Progress Report on Malting Barley Research March, 2002

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