Annual Progress Report on Malting Barley Research March, 2002

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136 Burns and R. Veseth (ed.) 2001 field day proceedings: Highlights of research progress. Dept. of Crop and Soil Sci. Tech. <strong>Report</strong> 01-4. WSU. Ullrich, S.E. 2001 Barley production and improvement in Washington State. Barley Newslttr. 44: http://wheat.pw.usda.gov/ggpages/BarleyNewsletter/44/Wash<strong>Report</strong>1.html. Schmierer, D., N. Kandemir, D. Kudrna, D. Wesenberg, S. Ullrich, and A. Kleinhofs. 2001. Molecular marker-assisted selection for increased yield of traditional malting barley cultivars. Barley Genetic Newslttr. 31:6-11. http://wheat.pw.usda.gov/ggpages/bgn/31/schmierer.htm. Ullrich, S.E., D. von Wettstein, J.W. Burns, and A.Kleinhofs. 2001. Spring barley performance in 2001. Wheat Life 44 (11): 50-54. Schmierer, D.*, N. Kandemir, D. Kudrna, D. Wesenberg, S.E. Ullrich, and A. Kleinhofs. 2001. Molecular marker-assisted selection for improved yield in traditional malting barley cultivars. Plant & Animal Genome IX International Conference (Jan. 13-17, San Diego). Final Abstracts Guide, p 163. Poster presentation. Kruger, C.I.*, R.J. Cook, W. Schillinger, V.A. Jitkov, R. Sloot, and S.E. Ullrich. 2001. Emergence characteristics of barley genotypes in a controlled environment with siolborne disease pressure. ASA-CSSA-SSSA <strong>Annual</strong> Meetings Abstracts CD-ROM. Oral Paper presented at the 2001 Western Society of Crop Science <strong>Annual</strong> Meetings, Tucson, AZ, 6/11-13/01. Hotchkin, W. S.*, S.C. Spaeth, V.A. Jikov, and S.E. Ullrich. 2001. Relationship between embryo morphology and stand establishment in hulless barley: breeding implications. ASA-CSSA-SSSA <strong>Annual</strong> Meetings Abstracts CD-ROM. Oral Paper presented at the 2001 Western Society of Crop Science <strong>Annual</strong> Meetings, Tucson, AZ, 6/11-13/01. Kruger, C.I.*, R.J. Cook, W. Schillinger, V. Jitkov, R. Sloot, and S.E. Ullrich. 2001. Stand establishment and performance characteristics of direct seeded barley genotypes. 2001 ASA-CSSA-SSSA <strong>Annual</strong> Meeting Abstracts CD-ROM. http://www.annualmeeting2001.com/Abstracts/c01-kruger143324-P.PDF. Poster presented at the 2001 ASA-CSSA-SSSA <strong>Annual</strong> Meetings, Charlotte, NC, 10/21-25/01. Voltas, J*., I. Romagosa, S.E. Ullrich, Van Eeuwijk, F.A. 2001. Identification of adaptive patterns in the ‘Steptoe’ X ‘Morex’ barley mapping population integrating genetic, phenotypic, and environmental information. 7 th International Quantitative Trait Locus Mapping and Marker-Assisted Selection Workshop. Valencia, Spain, 10/19-20/01. available at http://www.ivia.es/qtlmas/listados/modificar.php3?indice=26.
137 A STUDY OF THE MALTING QUALITY OF NEW BARLEY SELECTIONS Allen D. Budde, Berne L. Jones, Eddie Goplin and David M. Peterson Cereal Crops Research Unit, USDA, ARS and Department of Agronomy, University of Wisconsin - Madison Objectives. This project is an on going research collaboration with several state and federal malting barley breeding projects. Breeders' selections are micro-malted, analyzed and evaluated for malting quality. We can thereby provide accurate data and quality evaluations of new selections and thus facilitate the development of improved malting barleys. We also have carried out analyses that involve measuring the malting quality of experimental lines. These will lead to a better understanding of the biochemistry behind malting quality and/or will allow breeders to more effectively produce barley lines that have improved malting quality. Materials and methods. The methods used for the physical and chemical analyses of micro-malts are, for the most part, ASBC procedures. Some automated versions of the standard methods are used, in which case they have been standardized against the recommended procedures. Micro-malts are prepared with 170 g (db) or, when seed was limited, with 120 g samples. The samples are steeped to 45% moisture at 16ºC, germinated for 5 days at 17ºC, with turning for 3 min each half hour, and kilned over a period of 24 hours to a final temperature of 85ºC. These conditions are used to obtain malts having quality characteristics similar to those prepared commercially. All analytical data and evaluations of the micro-malts are summarized in individual reports that are returned to the breeders who had submitted the samples. At the end of the analysis year, the results are compiled into four annual regional reports that are posted at http://www.dfrc.ars.usda.gov/ccru/cc00001.html on our website. Data from the pilot scale analyses are submitted to AMBA for collaborative evaluation. Results. Barley samples brewed. We have discontinued the pilot brewing of lines that showed commercial potential, but are maintaining our brewing equipment in operating condition and sometimes use it for experimental studies. Malting and analyzing research samples. During the 2000 crop year, we malted and analyzed the quality of 993 samples (listed above, under ‘experimental’) for various USDA and non-USDA barley researchers, who needed malting quality information to carry out their research projects. These analyses were carried out in addition to analyzing samples that were submitted by breeders as part of their yearly breeding line quotas. Some of the research projects for which we analyzed samples were: 1) barleys that received various rates of nitrogen application; 2) lines that were developed and used to map the locations of QTLs on chromosomes; 3) proanthocyanidin-free barley lines; and 4) various projects being carried out at the CCRU.
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
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51 BARLEY TRANSFORMATION G.J. Muehl
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53 We have available the sugarcane
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55 Secondly, we tried to grow F. gr
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57 References: Issac, S. and Jennin
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59 Huntley-dryland) with two replic
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61 Table 2. 1992 thru 2001 Overall
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63 performance for Montana farmers.
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65 Blake, T,. Hensleigh P., Boss D.
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67 settling tower cannot accommodat
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Table 3. Seedling reaction to strip
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71 TWO-ROWED BARLEY IMPROVEMENT PRO
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Table 1. Agronomic trait comparison
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75 mutants reduce plant vigor and g
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77 SIX-ROWED BARLEY IMPROVEMENT PRO
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79 this variety has decreased over
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Table 7. Malt quality comparisons o
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83 • Management studies for malt
Page 93 and 94: 85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
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Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143: 135 D.M. Wesenberg - spring and win
Page 147 and 148: 139 Methods. Our malting schedule.
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
Page 161 and 162: 153 Sissons MJ and MacGregor AW (19
Page 163 and 164: µM Carbohydrate / Unit of α-Gluco
Page 165 and 166: 157 Tissue-specific gene products f
Page 167 and 168: 159 The promoters were subcloned fr
Page 169: 161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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