Annual Progress Report on Malting Barley Research March, 2002

7
6
5
4
3
2
1
0
56
Figure 1: Weight of Fusarium graminearum mycelium grown in liquid culture. Results are the
means ±SEM of duplicate measurements.
* indicates only one measurement made
Weight of Fusarium graminearum mycelium (g)
Control * 10 mM EDTA 1 mM EDTA 100 µM EDTA
Treatment
10 µM EDTA * 1 µM EDTA 100 nM EDTA
4. CHARACTERIZATION OF THE SCBV PROMOTER
From twelve T0 events, 5-10 T1 seeds were planted to produce T1 plants. These T1 plants were
analyzed for GUS expression. GUS was expressed highly in the roots, stems, leaves and all
reproductive tissues of the barley plants, the ovaries, anthers, bracts and rachis.
T2 seed from each of those twelve lines was then planted out and the T2 plants analyzed for GUS
expression. In eight of the lines, the expression remained constant, as it had in the previous
generation. However, in four of the lines, GUS expression was silenced. Nevertheless, these
data indicate that the ScBV promoter will be a useful promoter for transforming barley.
Future work - 1 st March 2002 - 30 th June 2002
Conlon stock plants are planted every two weeks. Therefore, an almost continuous supply of
explant material is available. In the remaining four months of this project, we will increase the
number of callus pieces bombarded with our barley glucanase gene from 40 to approximately
1000. We will also bombard approximately 1000 callus pieces with our barley RIP gene. The
success rate of barley transformation is approximately 1%, thus, 1000 callus pieces are necessary
to produce a significant number of transgenic events.
We will begin to characterize transgenic plants that are presently in the late stages of tissue
culture, utilizing our antibodies. We will begin to advance them to the next generation. We will
also test transgenics that express AFPs for in vitro antifungal effects.