Annual Progress Report on Malting Barley Research March, 2002

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70 RELATED PROJECTS Breeding materials and barley lines to be released in Montana have been screened for disease resistance in cooperation with the Montana Foundation Seed Program. Scald and net blotch populations are being monitored. No new virulence types have occurred over the last few years. Drought conditions have not been conducive to pathogen development in many parts of the state. Assistance was provides in regard to disease screening programs for barley stripe rust and other leaf diseases. We continue to maintain numerous leaf pathogens of barley. PUBLICATIONS Castro A., X. Chen, A. Corey, T. Fillichkin, P.M. Hayes, M. Johnston, S. Sandoval-Islas, and H. Vivar, 2002. Stripe rust resistance QTL pyramids in barley. In: Plant and Animal Genome X, San Diego, January 12-16 2002. Abstracts, p 179. Castro A., X. Chen, A. Corey, T. Fillichkin, P.M. Hayes, M. Johnston, S. Sandoval-Islas, and H. Vivar. Pyramiding of seedling barley stripe resistance QTL. In preparation for Crop Science
71 TWO-ROWED BARLEY IMPROVEMENT PROJECT Jerome D. Franckowiak Department of Plant Sciences North Dakota State University Fargo, ND 58105 Barley Breeding Objectives The primary objective of the barley breeding programs in the Agricultural Experiment Station, North Dakota State University (NDSU) is to develop and release improved barley cultivars for production in North Dakota (ND) and adjacent states. New cultivars should be acceptable to ND growers and those who use or process barley. Basic and applied research is conducted on barley to provide information that will aid achievement of the barley improvement goals, improve cultural practices, and enhance the understanding of barley genetics. This report emphasizes research on improvement of two-rowed barley for ND. The research activities of the two-rowed barley program are coordinated with the activities of other barley research projects at Fargo: the six-rowed barley improvement program, the barley quality program in the Department of Cereal Science, the barley pathology program in the Department of Plant Pathology, and the barley genetics program at the USDA-ARS Northern Crops Science Laboratory. General Seasonal Information Two-rowed barley cultivars and experimental lines were evaluated in yield trials grown at six ND locations during 2001. In addition the Carrington, Dickinson, Hettinger, Langdon, Minot, and Williston Research Extension Centers conducted yield trials at offstation sites. Pure seed increase plots of 34 entries in variety and advanced yield trials were grown in large drill strips at Casselton and Fargo. Pure seed increases of 17 historical cultivars and 66 experimental lines in intermediate yield trials were grown in small drill strips at Fargo. The barley yield trials were sown between 26 April and 15 May. The Western Spring, the Western Spring Dryland, and the Canadian Western Tworow cooperative barley nurseries were each grown at two locations. The yield trials were planted relatively early in 2001 and stand establishment was good at all locations. At Carrington, the herbicide Puma applied prior to several days of cold temperatures and caused differential damage to plots. Standing water in late June damaged plants in the Casselton, Fargo, and Prosper nurseries. Plots at Hettinger were destroyed by hail. Yield plots at Langdon were not harvested because of severe lodging and subsequent leaf diseases. Spot blotch, incited by Cochliobolus sativus, was severe in the nurseries at Casselton and Prosper. Spot blotch was also severe in several fields of ‘Harrington’ barley in the Minot area. The incidence of Fusarium head blight (FHB), caused mainly by Fusarium graminearum, was high in nurseries at Carrington and Langdon sites. Septoria leaf blotch, incited primarily by Septoria passerinii, were observed after heading in nurseries throughout ND. Net blotch, incited by Pyrenophora teres f. teres, were found on a few lines at Carrington and Minot. Notes on taken on lodging at Williston and grain protein values were estimated. Yields averaged about 75 bu/a from field plots at Fargo, 80 bu/a at Minot, and 90 bu/a at Williston.
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
Page 27 and 28: 19 Six-rowed barley selections of c
Page 29 and 30: 21 Table 14. Agronomic data for sel
Page 31 and 32: 23 Table 16. Agronomic data for win
Page 33 and 34: 25 Table 19. Malting quality data*
Page 35 and 36: Garnet/98Ab11865 Garnet/98Ab12895 9
Page 37 and 38: 29 rust resistant NSGC accessions o
Page 39 and 40: 31 DEVELOPMENT OF SIX-ROWED MALTING
Page 41 and 42: 33 backcrossing. The selfed lines w
Page 43 and 44: 35 MINNESOTA BARLEY IMPROVEMENT PRO
Page 45 and 46: 37 ADVANCED LINE EVALUATION Varieti
Page 47 and 48: 39 OTHER BARLEY DISEASES In 2001, r
Page 49 and 50: 41 Wingbermuehle, W. J., K.M. Belin
Page 51 and 52: 43 In 2001 winter/spring, over 300
Page 53 and 54: 45 assessments were made by countin
Page 55 and 56: 47 IDENTIFICATION OF NOVEL DISEASE
Page 57 and 58: 49 Unfortunately, disease levels in
Page 59 and 60: 51 BARLEY TRANSFORMATION G.J. Muehl
Page 61 and 62: 53 We have available the sugarcane
Page 63 and 64: 55 Secondly, we tried to grow F. gr
Page 65 and 66: 57 References: Issac, S. and Jennin
Page 67 and 68: 59 Huntley-dryland) with two replic
Page 69 and 70: 61 Table 2. 1992 thru 2001 Overall
Page 71 and 72: 63 performance for Montana farmers.
Page 73 and 74: 65 Blake, T,. Hensleigh P., Boss D.
Page 75 and 76: 67 settling tower cannot accommodat
Page 77: Table 3. Seedling reaction to strip
Page 81 and 82: Table 1. Agronomic trait comparison
Page 83 and 84: 75 mutants reduce plant vigor and g
Page 85 and 86: 77 SIX-ROWED BARLEY IMPROVEMENT PRO
Page 87 and 88: 79 this variety has decreased over
Page 89 and 90: Table 7. Malt quality comparisons o
Page 91 and 92: 83 • Management studies for malt
Page 93 and 94: 85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
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121 Table 7. Across location summar
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123 MOLECULAR MARKER ASSISTED MODIF
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125
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127 exchanges. Spring and winter ba
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129 Table 2. 2000-2001 Agronomic Pe
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131 Table 7. Spring 2- and 6-Row #
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133 2. Direct Seeding Cropping Syst
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135 D.M. Wesenberg - spring and win
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137 A STUDY OF THE MALTING QUALITY
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139 Methods. Our malting schedule.
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141 modification measures indicated
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143 CHARACTERIZATION OF THE PROTEIN
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145 Purification of a serine endope
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147 STUDIES ON THE PROTEINASES THAT
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149 with the proteinase T will be s
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151 10°C increase in thermostabili
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153 Sissons MJ and MacGregor AW (19
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µM Carbohydrate / Unit of α-Gluco
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157 Tissue-specific gene products f
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159 The promoters were subcloned fr
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161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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