Annual Progress Report on Malting Barley Research March, 2002

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84 Plant Sciences Personnel R.D. Horsley, Associate Professor and six-rowed barley breeder Marci Green, Research Specialist II Jeremy Pederson, Research Specialist II and M.S. Candidate Jason Faller, Ag. Research Technician III Gioconda Garcia, Ph.D. Candidate Ken Lamb, Ph.D. Candidate Publications Bolin, P., P. Schwarz, B. Jones, R. Horsley. 2001. Effect of malting parameters on the development of soluble protein in 6-rowed barley. ASBC Newsl. 61:PB24. Horsley, R.D., J.D. Franckowiak, P.B. Schwarz, and B.J. Steffenson. 2002. Registration of Drummond barley. Crop Sci. (accepted). Horsley, R.D., M.J. Wentz, and P.B. Schwarz. 2001. Relationships between common malt modification measurements. ASBC Newsl. 61:PB24. Rudd, J.C., R.D. Horsley, A.L. McKendry, and E. Elias. 2001. Host plant resistance genes for Fusarium head blight: sources, mechanisms, and utility in conventional breeding systems. Crop Sci. 41:620-627. Schwarz, P., J. Barr, M. Joyce, J. Power, and R. Horsley. 2002. Analysis of malt grist by manual sieve Test. J. Am. Soc. Brew. Chem. 60(1):10-13. Urrea, C.A., R.D. Horsley, and B.J. Steffenson. 2002. Heritability of Fusarium head blight resistance and deoxynivalenol accumulation from barley accession CIho 4196. Crop Sci. (accepted). Urrea, C.A., R.D. Horsley, B.J. Steffenson, and J.D. Franckowiak. 2002. Registration of 6NDRFG-1 six-rowed barley germplasm line with partial Fusarium head blight resistance. Crop Sci. (accepted).
85 STUDIES ON BARLEY DISEASES AND THEIR CONTROL Stephen Neate Department of Plant Pathology North Dakota State University Fargo, ND 58105 OBJECTIVES Diseases are important in limiting the yield and quality of malting barley production in the upper mid-west of the US. The objectives of the Barley Pathology Project at North Dakota State University is to investigate barley diseases and develop timely, practical methods for disease control to ensure that the quantity and quality of barley are not limited by disease. We aim to achieve this goal through the development of cultivars with genetic resistance, as well as development of cultural and chemical management strategies. To most efficiently achieve this goal it is also essential to investigate the population biology and basic ecology of pathogens that cause disease on barley. We have an ongoing program of monitoring and research to accomplish this goal. We also work closely with breeders to develop barley cultivars with multiple disease resistance. 1. Evaluate elite barley germplasm and segregating breeding populations for resistance to Fusarium Head blight (FHB). Working together with the breeders, Dr. J. Franckowiak, and Dr R. Horsley, NDSU, the barley pathology project has collaborated in identifying FHB resistance in approximately 2500 irrigated plots of barley accessions including Minndak, Foster/CIho4196, Foster/Bari, Elite, and transgenic populations. These accessions, as well as 9000 segregating breeding lines in plots of NDSU barley breeders were evaluated for FHB resistance. The resistant lines were subsequently evaluated for their FHB resistance in the greenhouse and the DON level of the inoculated kernels were determined. A summary of the results can be viewed on the US Wheat and Barley Scab Inititative web site. 2. Screen barley cultivars for resistance to spot blotch. A total of 600 barley entries was screened for spot blotch resistance to isolate SB85 of Cochliobolus sativus at the Fargo field site last year. Both the infection types and the percentage (0-100%) of leaf area affected by disease were evaluated. Among the tested 150 breeder’s lines, most of 6-rowed and 50% of 2-rowed lines were found to be resistant (with severity level
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
Page 41 and 42: 33 backcrossing. The selfed lines w
Page 43 and 44: 35 MINNESOTA BARLEY IMPROVEMENT PRO
Page 45 and 46: 37 ADVANCED LINE EVALUATION Varieti
Page 47 and 48: 39 OTHER BARLEY DISEASES In 2001, r
Page 49 and 50: 41 Wingbermuehle, W. J., K.M. Belin
Page 51 and 52: 43 In 2001 winter/spring, over 300
Page 53 and 54: 45 assessments were made by countin
Page 55 and 56: 47 IDENTIFICATION OF NOVEL DISEASE
Page 57 and 58: 49 Unfortunately, disease levels in
Page 59 and 60: 51 BARLEY TRANSFORMATION G.J. Muehl
Page 61 and 62: 53 We have available the sugarcane
Page 63 and 64: 55 Secondly, we tried to grow F. gr
Page 65 and 66: 57 References: Issac, S. and Jennin
Page 67 and 68: 59 Huntley-dryland) with two replic
Page 69 and 70: 61 Table 2. 1992 thru 2001 Overall
Page 71 and 72: 63 performance for Montana farmers.
Page 73 and 74: 65 Blake, T,. Hensleigh P., Boss D.
Page 75 and 76: 67 settling tower cannot accommodat
Page 77 and 78: Table 3. Seedling reaction to strip
Page 79 and 80: 71 TWO-ROWED BARLEY IMPROVEMENT PRO
Page 81 and 82: Table 1. Agronomic trait comparison
Page 83 and 84: 75 mutants reduce plant vigor and g
Page 85 and 86: 77 SIX-ROWED BARLEY IMPROVEMENT PRO
Page 87 and 88: 79 this variety has decreased over
Page 89 and 90: Table 7. Malt quality comparisons o
Page 91: 83 • Management studies for malt
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
Page 133 and 134: 125
Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
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135 D.M. Wesenberg - spring and win
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137 A STUDY OF THE MALTING QUALITY
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139 Methods. Our malting schedule.
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141 modification measures indicated
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143 CHARACTERIZATION OF THE PROTEIN
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145 Purification of a serine endope
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147 STUDIES ON THE PROTEINASES THAT
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149 with the proteinase T will be s
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151 10°C increase in thermostabili
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153 Sissons MJ and MacGregor AW (19
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µM Carbohydrate / Unit of α-Gluco
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157 Tissue-specific gene products f
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159 The promoters were subcloned fr
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161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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