Annual Progress Report on Malting Barley Research March, 2002

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110 resistance. Some of the lines show good resistance to the apparent new stripe rust virulence at Huancayo, Peru. We are focusing our winter malting barley efforts on genotypes that do not require vernalization. Results for our mapping lead us to hypothesize that in the development of 88ab536 there was either a crossover event leading to the separation of the genes that determine cold tolerance and vernalization on chromosome 7 (5H) or that an allelic architecture was created that leads to an epistatic down-regulation of the vernalization requirement. These hypotheses will be elaborated on in our proposal for continued funding. The immediate practical implication is that most of our winter malting barley selections can be spring-sown. This would offer a safety net to growers, in the event of extensive winter injury, and it could lead to the development of varieties with very broad adaptation. The yield of some of the winter selections under spring-sown conditions is competitive (Table 7). We hypothesize that the agronomic potential of these lines may be limited by the malting quality donor (Morex, via 88Ab536). Accordingly, as will be elaborated on in our proposal for continued funding, we propose to transfer winter hardiness QTL alleles to more recent Midwestern malting quality varieties and thus raise the bar of agronomic performance. Quality samples of the spring-sown winter malting selections were submitted, but data have not been received, so the consistency of quality expression across sowing dates is not known at this point. The malting quality of winter selections grown under fall-sown conditions at Pendleton, Pullman, and Aberdeen is shown in Table 6. For comparative purposes, we include the malting data from analyses of the same sample by different labs. Of these selections, STAB 7 is in accelerated seed increase in preparation for Plant Scale testing; STAB 7 and STAB 47 are currently both in a second year of the AMBA Pilot program; and STAB 113 is in a second year of first level testing in the AMBA Pilot program. KAB 47 and KAB 51 drill strips were planted at Pendleton and Aberdeen in Fall, 2001 in preparation for submission to the AMBA Pilot program. A notable feature of many of the winter malting selections is low grain protein, and this leads to lower than desired specs for other malt parameters. So, while much of the rest of the barley research community struggles with ways to lower grain protein, we are working on ways to increase it. In cooperation with S. Petrie and K. Rhinhart (Pendleton) we initiated a series of management trials in the fall of 2001. We are continuing with expanded versions of these trials this year and after this season, we will be able to present a comprehensive data analysis and a management package. At this point, it appears that we will be able to manage nitrogen (via split fall/spring applications) in order to achieve target levels of grain protein and enzymatic properties. However, the timing of the split applications will be critical, and there may be specific requirements for specific genotypes. Winter 6-row: 2001-2002 season Germplasm development Crosses are made at Corvallis and lines advanced to homozygosity through doubled haploid production, and/or single seed descent. With the emphasis on facultative growth habit, we are using SSD. With SSD, molecular markers can be used to indirectly select
111 for traits that are difficult/expensive to assess under field conditions. This selection during generation advance will increase the likelihood of developing lines with target profiles. With doubled haploids, one produces many lines without target profiles leading to a waste of agronomic and quality testing resources. Crosses: Based on performance of current winter lines under spring seeding we hypothesize that agronomic performance is limited by the spring parent (88ab536 – Morex). Accordingly, we reasoned that we should focus on converting spring types with better agronomic performance than Morex – e.g. Excel and Stander. We made crosses of STAB 7, 47, 113, Kab 47, Kab 51 with Excel and Stander. The advance of this germplasm will be the subject of our proposal for continued winter malting barley development. Germplasm advance: Progeny from the following cross combinations are currently being advanced via SSD in the greenhouse: STAB 7/ STAB 113; STAB113/STAB 47, KAB 43, and KAB 50; STAB 47/ KAB43, and KAB 51; STAB 7/KAB 43 and KAB 51. Cycle 1: Head rows The following SSD populations were planted in the fall of 2001 at Corvallis as F4 head rows: • STAB 113/Kab 43; Stab 113/Kab 50;Stab 113/Stab47; Stab 47/Kab 51; Stab 7/Kab 51; Stab 7/Kab 43; Stab 7/Stab 113; Stab 113/Stab 47 • Strider/Orca six-row lines (F7) selected for BYDV resistance • A set of winter barley top crosses Cycle 2. Single replication yield trials • All STAB white aleurone mapping population lines and parents; 140 entries. Cycle 3. Replicated yield trials • Selected STAB and KAB lines, plus checks at Pendleton, Oregon; Pullman, Washington; Aberdeen, Idaho. Cycle 4: Pre-release • AMBA Pilot scale drill strips: STAB 47, STAB 7, STAB 133, KAB 47; KAB 51 at Pendleton and Aberdeen • Management trials: Nitrogen, Chloride, Zinc, and date of planting at Pendleton and Moro. • On farm one acre trials: o STAB 113: Paul Williams at Davenport, WA o Stab 47: Bill Jepsen at Heppner, OR o Kab 37: Melvin Stonebrink at Wallowa, OR
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
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51 BARLEY TRANSFORMATION G.J. Muehl
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53 We have available the sugarcane
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55 Secondly, we tried to grow F. gr
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57 References: Issac, S. and Jennin
Page 67 and 68: 59 Huntley-dryland) with two replic
Page 69 and 70: 61 Table 2. 1992 thru 2001 Overall
Page 71 and 72: 63 performance for Montana farmers.
Page 73 and 74: 65 Blake, T,. Hensleigh P., Boss D.
Page 75 and 76: 67 settling tower cannot accommodat
Page 77 and 78: Table 3. Seedling reaction to strip
Page 79 and 80: 71 TWO-ROWED BARLEY IMPROVEMENT PRO
Page 81 and 82: Table 1. Agronomic trait comparison
Page 83 and 84: 75 mutants reduce plant vigor and g
Page 85 and 86: 77 SIX-ROWED BARLEY IMPROVEMENT PRO
Page 87 and 88: 79 this variety has decreased over
Page 89 and 90: Table 7. Malt quality comparisons o
Page 91 and 92: 83 • Management studies for malt
Page 93 and 94: 85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117: 109 THE OREGON BARLEY IMPROVEMENT P
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
Page 133 and 134: 125
Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143 and 144: 135 D.M. Wesenberg - spring and win
Page 145 and 146: 137 A STUDY OF THE MALTING QUALITY
Page 147 and 148: 139 Methods. Our malting schedule.
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
Page 161 and 162: 153 Sissons MJ and MacGregor AW (19
Page 163 and 164: µM Carbohydrate / Unit of α-Gluco
Page 165 and 166: 157 Tissue-specific gene products f
Page 167 and 168: 159 The promoters were subcloned fr
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161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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