Annual Progress Report on Malting Barley Research March, 2002

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Residual α-Glucosidase Activity 175 150 125 100 75 50 25 Mutant Wild Type 154 0 0 10 20 30 40 50 60 70 Temperature (°C) Figure 1. Effect of temperature on the activities of recombinant wild type (squares) and mutant (triangles) α-glucosidases at pH 6.0. Enzymes were incubated for 10 minutes at temperatures ranging from 0 to 60°C at pH 6. The residual rates of maltose hydrolysis were assayed for 18 hours at 30°C at pH 4.5. Residual activities are based on % of nonheated controls. Temperature (°C) 110 100 90 80 70 60 50 40 0 20 40 60 80 100 120 140 160 180 200 * Time (min) ** Adjunct Mash Main Mash Figure 2. Changes in mash temperature as a function of time. The adjunct mash (open circles) and main mash (closed circles) are shown. Samples for carbohydrate analysis were taken after protein rest (*) and after completion of starch conversion as determined by iodine testing (**).
µM Carbohydrate / Unit of α-Glucosidase Added 120 100 80 60 40 20 0 120 100 80 60 40 20 0 A B * Glucose * Glucose Maltose * Maltose WT T340P Maltotriose WT T340P * Maltotriose 155 µM Carbohydrate / Unit of α-Glucosidase Added 2.0 1.5 1.0 0.5 0.0 2.0 1.5 1.0 0.5 0.0 C D Maltotetraose Maltotetraose Maltopentaose Maltopentaose Maltohexaose Maltohexaose Maltoheptaose Maltoheptaose Figure 3. Effect of the addition of recombinant wild type (WT) or mutant (T340P) α-glucosidases on the production of fermentable (A,B) and non-fermentable (C,D) sugars during mashing. Carbohydrate concentrations were determined after protein rest (30 minutes at 46°C) (A,C) and after starch conversion (B,D). The * indicates significant differences between the means at the 0.05 level.
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
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25 Table 19. Malting quality data*
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Garnet/98Ab11865 Garnet/98Ab12895 9
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29 rust resistant NSGC accessions o
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31 DEVELOPMENT OF SIX-ROWED MALTING
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33 backcrossing. The selfed lines w
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35 MINNESOTA BARLEY IMPROVEMENT PRO
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37 ADVANCED LINE EVALUATION Varieti
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39 OTHER BARLEY DISEASES In 2001, r
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41 Wingbermuehle, W. J., K.M. Belin
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43 In 2001 winter/spring, over 300
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45 assessments were made by countin
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47 IDENTIFICATION OF NOVEL DISEASE
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49 Unfortunately, disease levels in
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51 BARLEY TRANSFORMATION G.J. Muehl
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53 We have available the sugarcane
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55 Secondly, we tried to grow F. gr
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57 References: Issac, S. and Jennin
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59 Huntley-dryland) with two replic
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61 Table 2. 1992 thru 2001 Overall
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63 performance for Montana farmers.
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65 Blake, T,. Hensleigh P., Boss D.
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67 settling tower cannot accommodat
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Table 3. Seedling reaction to strip
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71 TWO-ROWED BARLEY IMPROVEMENT PRO
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Table 1. Agronomic trait comparison
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75 mutants reduce plant vigor and g
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77 SIX-ROWED BARLEY IMPROVEMENT PRO
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79 this variety has decreased over
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Table 7. Malt quality comparisons o
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83 • Management studies for malt
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85 STUDIES ON BARLEY DISEASES AND T
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87 With funding from the US Wheat a
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89 MALTING AND BREWING QUALITY OF B
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91 β-glucans, it might be most ins
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10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
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95 Bolin, P., Schwarz, P., Jones, B
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97 Results Results from field and g
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99 DEVELOPING BARLEY TISSUE CULTURE
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101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
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Page 135 and 136: 127 exchanges. Spring and winter ba
Page 137 and 138: 129 Table 2. 2000-2001 Agronomic Pe
Page 139 and 140: 131 Table 7. Spring 2- and 6-Row #
Page 141 and 142: 133 2. Direct Seeding Cropping Syst
Page 143 and 144: 135 D.M. Wesenberg - spring and win
Page 145 and 146: 137 A STUDY OF THE MALTING QUALITY
Page 147 and 148: 139 Methods. Our malting schedule.
Page 149 and 150: 141 modification measures indicated
Page 151 and 152: 143 CHARACTERIZATION OF THE PROTEIN
Page 153 and 154: 145 Purification of a serine endope
Page 155 and 156: 147 STUDIES ON THE PROTEINASES THAT
Page 157 and 158: 149 with the proteinase T will be s
Page 159 and 160: 151 10°C increase in thermostabili
Page 161: 153 Sissons MJ and MacGregor AW (19
Page 165 and 166: 157 Tissue-specific gene products f
Page 167 and 168: 159 The promoters were subcloned fr
Page 169: 161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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