Annual Progress Report on Malting Barley Research March, 2002

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76 resistance from Chinese and Japanese barley cultivars. Mr. Guotai Yu is studying photoperiod response genes and FHB resistance genes in this material. Progenies will be grown in Hangzhou, China; Fargo, ND; and Yuma, AZ to evaluate photoperiod responses and to select desirable lines. Selected progenies and lines will be screened for FHB reactions in China and at Osnabrock, ND. They will also be evaluated for agronomic traits and malt quality parameters. 6. Early Maturity Genes in Midwest Barley. If recombination between the Eam6.h allele and QTLs for FHB resistance cannot be identified in Midwest malting barley, other maturity genes must be used. Initial experiments with other maturity genes suggest that genes controlling plant height should be revisited as barley lines adapted to the Upper Midwest are developed. The most promising maturity genes are Eam5.x from India via CIMMYT lines, eam9.l from China via Japan, and eam10.m from Russia via CIMMYT lines. In 2001 the eam9.l was found to interact with sdw1 dwarfing gene to produce relatively early semidwarf plants. The Eam5.x may show a similar interaction with the sdw1 gene. If these observations can be verified, utilization of the sdw1 gene in development barley cultivars for ND may be possible. Personnel in the Department of Plant Sciences Jerome D. Franckowiak, Professor and two-rowed barley breeder. Roger C. Genoch, Research Technician III. Manju Kathikeyan, an M.S. degree candidate from India. Nadejda Krasheninnik, a Ph.D. degree candidate from Russia. Guotai Yu, a Ph.D. degree candidate from China. Publications Franckowiak, J.D. 2001. Accumulating genes for disease resistance in two-rowed barley for North Dakota. p. 39-46. In H.E. Vivar and A. McNab (eds.). Breeding Barley in the New Millennium: Proceedings of an International Symposium. CIMMYT, Mexico., D.F. Franckowiak, J.D. 2001. Coordinator’s report: Chromosome 2H (2). Barley Genet. Newsl. 31: http://wheat.pw.usda.gov/ggpages/bgn/31/ul1txt.htm#2H. Franckowiak, J.D. 2001. Coordinator’s report: Semidwarf genes. Barley Genet. Newsl. 31: http://wheat.pw.usda.gov/ggpages/bgn/31/ul1txt.htm#semidwarf. Franckowiak, J.D., and R.D. Horsley. 2001. Development of scab resistant barley varieties for North Dakota. p. 14-20. In Proc. 33 rd . Barley Improvement Conference. American Malting Barley Assoc., Milwaukee, WI.
77 SIX-ROWED BARLEY IMPROVEMENT PROJECT Richard D. Horsley Department of Plant Sciences North Dakota State University Objectives The six-rowed barley improvement research program at the Agricultural Experiment Station, North Dakota State University, Fargo, is a cooperative effort among the Departments of Plant Sciences, Cereal and Food Sciences, and Plant Pathology. The fundamental objective of the program is to develop and release improved six-rowed barley cultivars acceptable to barley producers in North Dakota and adjacent areas in the United States, and to those who use or process this barley. Basic and applied research is conducted at NDSU on barley to provide information that will facilitate achievement of the barley improvement goals, improve cultural practices, and enhance our understanding of barley. Barley Breeding Advanced Testing Program - General Important commercial barley cultivars, new cultivars, and promising advanced selections were evaluated in ten trials at seven experimental locations in North Dakota during 2001. In addition, "off-station" trials with new and check cultivars were conducted by the Carrington, Dickinson, Hettinger, Langdon, North Central (Minot), and Williston Research Extension Centers. Pure seed increase plots of experimental lines (26) in Varietal and Advanced Yield Trials were grown at Fargo and Casselton. Pure seed increase plots of experimental lines (44) in the Intermediate Malting Barley Yield Trial were grown at Fargo. Barley yield trials at all locations were sown between 26 April and 17 May. Sowing at all locations was about seven to ten days later than average due to wet soil conditions. Regional trials grown were the Mississippi Valley Barley Nursery and the Canadian Western Cooperative Six-row Trial. Plant emergence in yield trials was fairly uniform at all locations. As in recent years, growing conditions during the two halves of the growing season varied drastically. Precipitation from sowing up to mid-June was below average. These conditions limited the yield potential of the crop, especially in northeastern North Dakota. From mid-June to mid-July, precipitation was at levels that favored development of Fusarium head blight (FHB). Several back-to-back rain storms in July with high winds caused extensive lodging in all small grain crops grown in northeast North Dakota. The major disease problem occurring on barley produced in northeastern and northcentral North Dakota was FHB, incited primarily by Fusarium graminearum. Severity of FHB was minimal at Fargo, and moderate to high at Carrington, Osnabrock, and Minot. Levels of FHB at Minot were the greatest observed since the epidemic began in 1993. Overall, the percent of FHB found in our yield trial nurseries in 2001 was slightly less than that in 2000. The most disturbing aspect of this disease is that it continues to move west in the state into areas where it was not a problem previously. All cultivars grown in
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Annual Pro
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-i- INTRODUCTION The March 2002 <st
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Barley Transformation G.J. Muehlbau
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1 2000/2001 WINTER NURSERY: BARLEY
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3 2001 Barley Stripe Rust Screening
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5 Triumph/Tyra//Arupo, a selection
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7 Washington (Fossum Cereals and Wa
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9 This project is supported by the
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11 ‘Triumph’, ‘Valier’, ‘
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13 Table 2. Agronomic data for sele
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15 Table 4. Agronomic data for sele
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17 Table 8. Malting quality data* f
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19 Six-rowed barley selections of c
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21 Table 14. Agronomic data for sel
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23 Table 16. Agronomic data for win
Page 33 and 34: 25 Table 19. Malting quality data*
Page 35 and 36: Garnet/98Ab11865 Garnet/98Ab12895 9
Page 37 and 38: 29 rust resistant NSGC accessions o
Page 39 and 40: 31 DEVELOPMENT OF SIX-ROWED MALTING
Page 41 and 42: 33 backcrossing. The selfed lines w
Page 43 and 44: 35 MINNESOTA BARLEY IMPROVEMENT PRO
Page 45 and 46: 37 ADVANCED LINE EVALUATION Varieti
Page 47 and 48: 39 OTHER BARLEY DISEASES In 2001, r
Page 49 and 50: 41 Wingbermuehle, W. J., K.M. Belin
Page 51 and 52: 43 In 2001 winter/spring, over 300
Page 53 and 54: 45 assessments were made by countin
Page 55 and 56: 47 IDENTIFICATION OF NOVEL DISEASE
Page 57 and 58: 49 Unfortunately, disease levels in
Page 59 and 60: 51 BARLEY TRANSFORMATION G.J. Muehl
Page 61 and 62: 53 We have available the sugarcane
Page 63 and 64: 55 Secondly, we tried to grow F. gr
Page 65 and 66: 57 References: Issac, S. and Jennin
Page 67 and 68: 59 Huntley-dryland) with two replic
Page 69 and 70: 61 Table 2. 1992 thru 2001 Overall
Page 71 and 72: 63 performance for Montana farmers.
Page 73 and 74: 65 Blake, T,. Hensleigh P., Boss D.
Page 75 and 76: 67 settling tower cannot accommodat
Page 77 and 78: Table 3. Seedling reaction to strip
Page 79 and 80: 71 TWO-ROWED BARLEY IMPROVEMENT PRO
Page 81 and 82: Table 1. Agronomic trait comparison
Page 83: 75 mutants reduce plant vigor and g
Page 87 and 88: 79 this variety has decreased over
Page 89 and 90: Table 7. Malt quality comparisons o
Page 91 and 92: 83 • Management studies for malt
Page 93 and 94: 85 STUDIES ON BARLEY DISEASES AND T
Page 95 and 96: 87 With funding from the US Wheat a
Page 97 and 98: 89 MALTING AND BREWING QUALITY OF B
Page 99 and 100: 91 β-glucans, it might be most ins
Page 101 and 102: 10 9 8 7 6 5 4 3 2 1 0 93 Figure 1.
Page 103 and 104: 95 Bolin, P., Schwarz, P., Jones, B
Page 105 and 106: 97 Results Results from field and g
Page 107 and 108: 99 DEVELOPING BARLEY TISSUE CULTURE
Page 109 and 110: 101 trials of plants regenerated fr
Page 111 and 112: 103 Table 2. Agronomic performance
Page 113 and 114: 105 BSMV spread between entries. Ho
Page 115 and 116: 107 involving two susceptible backg
Page 117 and 118: 109 THE OREGON BARLEY IMPROVEMENT P
Page 119 and 120: 111 for traits that are difficult/e
Page 121 and 122: Germplasm Development: 113 Crosses:
Page 123 and 124: 115 • All lines in spring yield t
Page 125 and 126: 117 Table 2. Agronomic data for Ore
Page 127 and 128: 119 Table 6. Malting quality of win
Page 129 and 130: 121 Table 7. Across location summar
Page 131 and 132: 123 MOLECULAR MARKER ASSISTED MODIF
Page 133 and 134: 125
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127 exchanges. Spring and winter ba
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129 Table 2. 2000-2001 Agronomic Pe
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131 Table 7. Spring 2- and 6-Row #
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133 2. Direct Seeding Cropping Syst
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135 D.M. Wesenberg - spring and win
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137 A STUDY OF THE MALTING QUALITY
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139 Methods. Our malting schedule.
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141 modification measures indicated
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143 CHARACTERIZATION OF THE PROTEIN
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145 Purification of a serine endope
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147 STUDIES ON THE PROTEINASES THAT
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149 with the proteinase T will be s
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151 10°C increase in thermostabili
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153 Sissons MJ and MacGregor AW (19
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µM Carbohydrate / Unit of α-Gluco
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157 Tissue-specific gene products f
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159 The promoters were subcloned fr
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161 Fig. 3. Expression of Trx-Hth f
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Annual Progress Report on Malting Barley Research March, 2002

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