Thesis - faculty.ait.ac.th - Asian Institute of Technology
Thesis - faculty.ait.ac.th - Asian Institute of Technology
Thesis - faculty.ait.ac.th - Asian Institute of Technology
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Figure 3.7 Flowchart Showing Ammonia Stripping Coupled MBR Process<br />
3.8 Analytical Me<strong>th</strong>ods<br />
Raw Le<strong>ac</strong>hate<br />
Ammonia Stripping<br />
Membrane Biore<strong>ac</strong>tor using B<strong>ac</strong>terial and<br />
Yeast Cultures<br />
The analyses performed were in <strong>ac</strong>cordance wi<strong>th</strong> <strong>th</strong>e Standard Me<strong>th</strong>ods (APHA, et<br />
al., 1998). Table 3.8 lists parameters and <strong>th</strong>eir analysis me<strong>th</strong>ods in <strong>th</strong>is study.<br />
Extr<strong>ac</strong>tion <strong>of</strong> Extr<strong>ac</strong>ellular Polymeric Substances (EPS)<br />
The quantification <strong>of</strong> EPS in biomass was analyzed using <strong>th</strong>ermal extr<strong>ac</strong>tion me<strong>th</strong>od<br />
(Chang and Lee, 1998). A measured volume <strong>of</strong> sludge solid was centrifuged in order to<br />
subtr<strong>ac</strong>t <strong>th</strong>e soluble EPS at 3,200 rpm for 30 min from bound EPS. After collecting <strong>th</strong>e<br />
soluble EPS, <strong>th</strong>e remaining pellet was resuspended wi<strong>th</strong> 0.9% NaCl solution before heating<br />
at 80 o C for 1 h. The extr<strong>ac</strong>ted solution was separated from <strong>th</strong>e sludge solids by<br />
centrifugation at 3,200 rpm for 30 min. The obtained supernatant was <strong>th</strong>e bound EPS. The<br />
quantity <strong>of</strong> extr<strong>ac</strong>ted EPS was measured by measuring total organic carbon (TOC),<br />
proteins and carbohydrates.<br />
65<br />
Operating Conditions:<br />
- pH<br />
- Velocity Gradient<br />
- Operation Time
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