credits bmbl December 7 '06.doc - Central Michigan University

More documents

Recommendations

Info

Vertebrate Animal Biosafety Level Criteria – Animal Biosafety Level 4 Personal health status may impact an individual’s susceptibility to infection, ability to receive immunizations or prophylactic interventions. Therefore, all laboratory personnel and particularly women of child-bearing age should be provided with information regarding immune competence and conditions that may predispose them to infection. Individuals having these conditions should be encouraged to self-identify to the institution’s healthcare provider for appropriate counseling and guidance. 2. Animal facility personnel and support staff must be provided occupational medical services, including medical surveillance and available immunizations for agents handled or potentially present in the laboratory. A system must be established for reporting and documenting laboratory accidents, exposures, employee absenteeism and for the medical surveillance of potential laboratory-acquired illnesses. An essential adjunct to an occupational medical system is the availability of a facility for the isolation and medical care of personnel with potential or known laboratory-acquired illnesses. 3. Each institution must establish policies and procedures describing the collection and storage of serum samples from at-risk personnel. 4. The animal facility supervisor is responsible for ensuring that animal personnel: a. Receive appropriate training in the practices and operations specific to the animal facility, such as animal husbandry procedures, potential hazards present, manipulations of infectious agents, necessary precautions to prevent potential exposures. b. Demonstrate high proficiency in standard and special microbiological practices, and techniques before entering the ABSL-4 facility or working with agents requiring BSL-4 containment. c. Receive annual updates and additional training when procedure or policy changes occur. Records are maintained for all hazard evaluations and employee training. 5. Removal of biological materials that are to remain in a viable or intact state from the ABSL-4 laboratory must be transferred to a non-breakable, sealed primary container and then enclosed in a non-breakable, sealed secondary container. These materials must be transferred through a disinfectant dunk tank, fumigation chamber, or decontamination shower. Once removed, packaged viable material must not be opened outside BSL-4 containment unless inactivated by a validated method. 6. Laboratory equipment must be routinely decontaminated, as well as after spills, splashes, or other potential contamination. Equipment, cages, and racks 106
Vertebrate Animal Biosafety Level Criteria – Animal Biosafety Level 4 should be handled in manner that minimizes contamination of other areas. Cages are autoclaved or thoroughly decontaminated before they are cleaned and washed. a. All equipment and contaminated materials must be decontaminated before removal from the animal facility. Equipment must be decontaminated using an effective and validated method before repair, maintenance, or removal from the animal facility. b. Equipment or material that might be damaged by high temperatures or steam must be decontaminated using an effective and validated procedure such as gaseous or vapor method in an airlock or chamber designed for this purpose. c. Spills involving infectious materials must be contained, decontaminated, and cleaned up by staff properly trained and equipped to work with infectious material. A spill procedure must be developed and posted within the laboratory. Spills and accidents of potentially infectious materials must be immediately reported to the animal facility and laboratory supervisors or personnel designated by the institution. 7. The doors of the autoclave and fumigation chamber are interlocked in a manner that prevents opening of the outer door unless the autoclave/decontamination chamber has been operated through a decontamination cycle or the fumigation chamber has been decontaminated. 8. Daily inspections of essential containment and life support systems must be completed before laboratory work is initiated to ensure that the laboratory and animal facilities are operating according to its established parameters. 9. Practical and effective protocols for emergency situations must be established. These protocols must include plans for medical emergencies, facility malfunctions, fires, escape of animals within the ABSL-4 laboratory, and other potential emergencies. Training in emergency response procedures must be provided to emergency response personnel according to institutional policies. 10. Based on site-specific risk assessment, personnel assigned to work with infected animals may be required to work in pairs. Procedures to reduce possible worker exposure must be instituted, such as use of squeeze cages, working only with anesthetized animals, or other appropriate practices. C. Safety Equipment (Primary Barriers and Personal Protective Equipment) Cabinet Laboratory 107
Page 1 and 2:
Biosafety in Microbiological and Bi
Page 3 and 4:
SECTION VIII-H: Prion Diseases 299
Page 5 and 6:
Forward Biosafety in Microbiologica
Page 7 and 8:
Editors: L. Casey Chosewood, MD Dir
Page 9 and 10:
Guest Editors: Matthew J. Arduino,
Page 11 and 12:
Charles B. Millard, PhD Lieutenant
Page 13 and 14:
Robert Bull Karen B. Byers Jane Cap
Page 15 and 16:
Thomas L. Miller Douglas M. Moore M
Page 17 and 18:
Robert Yarchoan Uri Yokel Lisa Youn
Page 19 and 20:
Introduction laboratories chose not
Page 21 and 22:
Introduction RISK CRITERIA FOR ESTA
Page 23 and 24:
Introduction BMBL includes an impor
Page 25 and 26:
Section II Biological Risk Assessme
Page 27 and 28:
Biological Risk Assessment informat
Page 29 and 30:
Biological Risk Assessment The NIH
Page 31 and 32:
Biological Risk Assessment effectiv
Page 33 and 34:
Biological Risk Assessment Third, m
Page 35 and 36:
Biological Risk Assessment 14. Oxma
Page 37 and 38:
Principles of Biosafety Safety equi
Page 39 and 40:
Principles of Biosafety Often an in
Page 41 and 42:
Principles of Biosafety Four standa
Page 43 and 44:
Principles of Biosafety 4. Centers
Page 45 and 46:
Laboratory Biosafety Level Criteria
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56: Laboratory Biosafety Level Criteria
Page 75 and 76: Section V Vertebrate Animal Biosafe
Page 77 and 78: Vertebrate Animal Biosafety Level C
Page 105: Vertebrate Animal Biosafety Level C
Page 119 and 120: TABLE 1 SUMMARY OF RECOMMENDED BIOS
Page 121 and 122: Principles of Laboratory Biosecurit
Page 131 and 132: Occupational Health and Immunoproph
Page 139 and 140: Agent Summary Statements - Bacteria
Page 157 and 158:
Agent Summary Statements - Bacteria
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
• Section VIII-B: Fungal Agents A
Page 187 and 188:
Agent Summary Statements - Fungal A
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
• Section VIII-C: Parasitic Agent
Page 199 and 200:
Agent Summary Statements - Parasiti
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
• Section VIII-D: Rickettsial Age
Page 211 and 212:
Agent Summary Statements - Ricketts
Page 213 and 214:
Agent Summary Statements - Ricketts
Page 215 and 216:
• Section VIII-E: Viral Agents Ag
Page 217 and 218:
Agent Summary Statements - Viral Ag
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Agent Summary Statements - Arboviru
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Page 267 and 268:
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
• Section VIII-G: Toxin Agents Se
Page 287 and 288:
Agent Summary Statements - Toxins i
Page 289 and 290:
Agent Summary Statements - Toxins A
Page 291 and 292:
Agent Summary Statements - Toxins L
Page 293 and 294:
Agent Summary Statements - Toxins e
Page 295 and 296:
Agent Summary Statements - Toxins o
Page 297 and 298:
Agent Summary Statements - Toxins t
Page 299 and 300:
• Section VIII-H: Prion Diseases
Page 301 and 302:
Agent Summary Statement - Prion Dis
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Appendix A Proper maintenance of ca
Page 309 and 310:
Appendix A declined. However, in ma
Page 311 and 312:
Appendix A It is possible to exhaus
Page 313 and 314:
Appendix A 4. The Class II, Type A2
Page 315 and 316:
Appendix A prohibited. Furthermore,
Page 317 and 318:
Appendix A plastic wrappers, pipett
Page 319 and 320:
Appendix A touch-plate microburners
Page 321 and 322:
Appendix A SECTION VI FACILITY AND
Page 323 and 324:
Appendix A Development of Containme
Page 325 and 326:
Appendix A C. Airflow Smoke Pattern
Page 327 and 328:
Appendix A TABLES Table 1. Selectio
Page 329 and 330:
Appendix A Table 3. Field Performan
Page 331 and 332:
Appendix A FIGURES Figure 1. HEPA f
Page 333 and 334:
Appendix A Figure 3. The Class II,
Page 335 and 336:
Appendix A Figure 5B. The Class II,
Page 337 and 338:
Appendix A Figure 8. The Class III
Page 339 and 340:
Appendix A Figure 9B. The vertical
Page 341 and 342:
Appendix A Figure 11. A typical lay
Page 343 and 344:
Appendix A REFERENCES 1. Centers fo
Page 345 and 346:
Appendix B Decontamination and Disi
Page 347 and 348:
Appendix B surfaces are contaminate
Page 349 and 350:
Appendix B TABLE 1 DESCENDING ORDER
Page 351 and 352:
Appendix B dioxide gas exits the ge
Page 353 and 354:
Appendix B d The effectiveness of a
Page 355 and 356:
Appendix C Transportation of Infect
Page 357 and 358:
Appendix C 1600 Clifton Road, N.E.,
Page 359 and 360:
Appendix C disease in humans or in
Page 361 and 362:
Appendix C Biological specimen, Cat
Page 363 and 364:
Appendix D Agriculture Pathogen Bio
Page 365 and 366:
Appendix D simultaneous opening of
Page 367 and 368:
Appendix D 14. Pathological inciner
Page 369 and 370:
Appendix D Wastes and other materia
Page 371 and 372:
Appendix D Camelpox virus b a, b, c
Page 373 and 374:
Appendix D SPECIAL ISSUES The impor
Page 375 and 376:
Appendix D required by the USDA and
Page 377 and 378:
Appendix D depression, anorexia, an
Page 379 and 380:
Appendix D laboratory with enhancem
Page 381 and 382:
Appendix D five day period of conta
Page 383 and 384:
Appendix D slaughterhouses or indus
Page 385 and 386:
Appendix D virus does not cause any
Page 387 and 388:
Appendix D NDV is classified in the
Page 389 and 390:
Appendix D movement permit is requi
Page 391 and 392:
Appendix D Spring Viremia of Carp V
Page 393 and 394:
Appendix D REFERENCES 1. Hess WR. A
Page 395 and 396:
Appendix D 37. Alexander DJ. Newcas
Page 397 and 398:
Appendix E Arthropod Containment Gu
Page 399 and 400:
Appendix F Select Agents and Toxins
Page 401 and 402:
Appendix G IPM issues and requireme
Page 403 and 404:
Appendix H Working with Human, NHP
Page 405 and 406:
Appendix I Guidelines for Work with
Page 407 and 408:
decontaminated periodically, for ex
Page 409 and 410:
0.25N, and/or sodium hypochlorite (
Page 411 and 412:
k Irradiation causes a dose-depende
Page 413 and 414:
7. Morin R, Kozlovac J. Biological
Page 415 and 416:
Appendix J NIH Oversight of Researc
Page 417 and 418:
Appendix K Resources for Informatio
Page 419 and 420:
Appendix K E-mail: http://www2.gsu.
Page 421 and 422:
Appendix K and http://www.who.int/c
Page 423 and 424:
Appendix L HEPA High Efficiency Par
show all

credits bmbl December 7 '06.doc - Central Michigan University

Create successful ePaper yourself

Delete template?

Save as template?