credits bmbl December 7 '06.doc - Central Michigan University

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Vertebrate Animal Biosafety Level Criteria – Animal Biosafety Level 4 9. A dedicated non-recirculating ventilation system is provided. Only laboratories with the same HVAC requirements (i.e., other BSL-4 labs, ABSL-4, BSL-3 Ag labs) may share ventilation systems if each individual laboratory system is isolated by gas tight dampers and HEPA filters. The supply and exhaust components of the ventilation system must be designed to maintain the ABSL-4 laboratory at negative pressure to surrounding areas and provide differential pressure/directional airflow between adjacent areas within the laboratory. Redundant supply fans are recommended. Redundant exhaust fans are required. Supply and exhaust fans must be interlocked to prevent positive pressurization of the laboratory. The ventilation system must be monitored and alarmed to indicate malfunction or deviation from design parameters. A visual monitoring device must be installed near the clean change room so proper differential pressures within the laboratory may be verified. Supply air to and exhaust air from the cabinet room, inner change room, and fumigation/decontamination chambers must pass through HEPA filter(s). The air exhaust discharge must be located away from occupied spaces and building air intakes. All HEPA filters should be located as near as practicable to the cabinet laboratory in order to minimize the length of potentially contaminated ductwork. All HEPA filters must be tested and certified annually. The HEPA filter housings should be designed to allow for in situ decontamination and validation of the filter prior to removal. The design of the HEPA filter housing must have gas-tight isolation dampers; decontamination ports, and ability to scan each filter assembly for leaks. 10. HEPA filtered exhaust air from a Class II BSC can be safely re-circulated into the laboratory environment if the cabinet is tested and certified at least annually and operated according to the manufacturer’s recommendations. Biological safety cabinets can also be connected to the laboratory exhaust system by either a thimble (canopy) connection or a direct (hard) connection. Provisions to assure proper safety cabinet performance and air system operation must be verified. Class III BSCs must be directly and independently exhausted through two HEPA filters in series. Supply air must be provided in such a manner that prevents positive pressurization of the cabinet. 112
Vertebrate Animal Biosafety Level Criteria – Animal Biosafety Level 4 11. Pass through dunk tanks, fumigation chambers, or equivalent decontamination methods must be provided so that materials and equipment that cannot be decontaminated in the autoclave can be safely removed from the cabinet room(s). Access to the exit side of the pass though shall be limited to those individuals authorized to be in the ABSL-4 laboratory. 12. Liquid effluents from cabinet room sinks, floor drains, autoclave chambers, and other sources within the cabinet room must be decontaminated by a proven method, preferably heat treatment, before being discharged to the sanitary sewer. Decontamination of all liquid wastes must be documented. The decontamination process for liquid wastes must be validated physically and biologically. Biological validation must be performed annually or more often as required by institutional policy. Effluents from showers and toilets may be discharged to the sanitary sewer without treatment. 13. A double-door autoclave must be provided for decontaminating waste or other materials passing out of the cabinet room. Autoclaves that open outside of the laboratory must be sealed to the wall. This bioseal must be durable, airtight, and sealed to the wall. Positioning the bioseal so that the equipment can be accessed and maintained from outside the laboratory is recommended. The autoclave doors must be interlocked so that only one can be opened at any time and be automatically controlled so that the outside door can only be opened after the autoclave decontamination cycle has been completed. Gas and liquid discharge from the autoclave chamber must be decontaminated. When feasible, autoclave decontamination processes should be designed so that over-pressurization cannot release unfiltered air or steam exposed to infectious material to the environment. 14. The ABSL-4 facility design parameters and operational procedures must be documented. The facility must be tested to verify that the design and operational parameters have been met prior to operation. Facilities must also be re-verified annually. Verification criteria should be modified as necessary by operational experience. 15. Appropriate communication systems must be provided between the ABSL-4 laboratory and the outside (e.g., voice, fax, and computer). Provisions for emergency communication and access/egress must be considered. Suit Laboratory 113
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Biosafety in Microbiological and Bi
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SECTION VIII-H: Prion Diseases 299
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Forward Biosafety in Microbiologica
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Editors: L. Casey Chosewood, MD Dir
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Guest Editors: Matthew J. Arduino,
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Charles B. Millard, PhD Lieutenant
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Robert Bull Karen B. Byers Jane Cap
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Thomas L. Miller Douglas M. Moore M
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Robert Yarchoan Uri Yokel Lisa Youn
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Introduction laboratories chose not
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Introduction RISK CRITERIA FOR ESTA
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Introduction BMBL includes an impor
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Section II Biological Risk Assessme
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Biological Risk Assessment informat
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Biological Risk Assessment The NIH
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Biological Risk Assessment effectiv
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Biological Risk Assessment Third, m
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Biological Risk Assessment 14. Oxma
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Principles of Biosafety Safety equi
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Principles of Biosafety Often an in
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Principles of Biosafety Four standa
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Principles of Biosafety 4. Centers
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Laboratory Biosafety Level Criteria
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Page 61 and 62: Laboratory Biosafety Level Criteria
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Page 119 and 120: TABLE 1 SUMMARY OF RECOMMENDED BIOS
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Agent Summary Statements - Bacteria
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• Section VIII-B: Fungal Agents A
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Agent Summary Statements - Fungal A
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• Section VIII-C: Parasitic Agent
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Agent Summary Statements - Parasiti
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• Section VIII-D: Rickettsial Age
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Agent Summary Statements - Ricketts
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Agent Summary Statements - Ricketts
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• Section VIII-E: Viral Agents Ag
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Agent Summary Statements - Viral Ag
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Agent Summary Statements - Arboviru
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• Section VIII-G: Toxin Agents Se
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Agent Summary Statements - Toxins i
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Agent Summary Statements - Toxins A
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Agent Summary Statements - Toxins L
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Agent Summary Statements - Toxins e
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Agent Summary Statements - Toxins o
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Agent Summary Statements - Toxins t
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• Section VIII-H: Prion Diseases
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Agent Summary Statement - Prion Dis
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Appendix A Proper maintenance of ca
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Appendix A declined. However, in ma
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Appendix A It is possible to exhaus
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Appendix A 4. The Class II, Type A2
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Appendix A prohibited. Furthermore,
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Appendix A plastic wrappers, pipett
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Appendix A touch-plate microburners
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Appendix A SECTION VI FACILITY AND
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Appendix A Development of Containme
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Appendix A C. Airflow Smoke Pattern
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Appendix A TABLES Table 1. Selectio
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Appendix A Table 3. Field Performan
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Appendix A FIGURES Figure 1. HEPA f
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Appendix A Figure 3. The Class II,
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Appendix A Figure 5B. The Class II,
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Appendix A Figure 8. The Class III
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Appendix A Figure 9B. The vertical
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Appendix A Figure 11. A typical lay
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Appendix A REFERENCES 1. Centers fo
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Appendix B Decontamination and Disi
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Appendix B surfaces are contaminate
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Appendix B TABLE 1 DESCENDING ORDER
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Appendix B dioxide gas exits the ge
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Appendix B d The effectiveness of a
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Appendix C Transportation of Infect
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Appendix C 1600 Clifton Road, N.E.,
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Appendix C disease in humans or in
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Appendix C Biological specimen, Cat
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Appendix D Agriculture Pathogen Bio
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Appendix D simultaneous opening of
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Appendix D 14. Pathological inciner
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Appendix D Wastes and other materia
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Appendix D Camelpox virus b a, b, c
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Appendix D SPECIAL ISSUES The impor
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Appendix D required by the USDA and
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Appendix D depression, anorexia, an
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Appendix D laboratory with enhancem
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Appendix D five day period of conta
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Appendix D slaughterhouses or indus
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Appendix D virus does not cause any
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Appendix D NDV is classified in the
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Appendix D movement permit is requi
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Appendix D Spring Viremia of Carp V
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Appendix D REFERENCES 1. Hess WR. A
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Appendix D 37. Alexander DJ. Newcas
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Appendix E Arthropod Containment Gu
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Appendix F Select Agents and Toxins
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Appendix G IPM issues and requireme
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Appendix H Working with Human, NHP
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Appendix I Guidelines for Work with
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decontaminated periodically, for ex
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0.25N, and/or sodium hypochlorite (
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k Irradiation causes a dose-depende
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7. Morin R, Kozlovac J. Biological
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Appendix J NIH Oversight of Researc
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Appendix K Resources for Informatio
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Appendix K E-mail: http://www2.gsu.
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Appendix K and http://www.who.int/c
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Appendix L HEPA High Efficiency Par
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credits bmbl December 7 '06.doc - Central Michigan University

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