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credits bmbl December 7 '06.doc - Central Michigan University

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Appendix A<br />

Figure 11. A typical layout for working “clean to dirty” within a Class II BSC. Clean<br />

cultures (left) can be inoculated (center); contaminated pipettes can be discarded in the<br />

shallow pan and other contaminated materials can be placed in the biohazard bag (right).<br />

This arrangement is reversed for left-handed persons.<br />

Figure 12. One method to protect a house vacuum system during aspiration of<br />

infectious fluids. The left suction flask (A) is used to collect the contaminated fluids into<br />

a suitable decontamination solution; the right flask serves as a fluid overflow collection<br />

vessel. A glass sparger in flask B minimizes splatter. An in-line HEPA filter (C) is used<br />

to protect the vacuum system (D) from aerosolized microorganisms.<br />

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