credits bmbl December 7 '06.doc - Central Michigan University

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Appendix A Table 2. Comparison of Biosafety Cabinet Characteristics BSC Class Face Velocity Airflow Pattern Nonvolatile Toxic Chemicals and Radionuclides In at front through HEPA to the I 75 outside or into the room through Yes II, A1 75 II, B1 100 II, B2 100 II, A2 100 III N/A HEPA (figure 2) 70% recirculated to the cabinet work area through HEPA; 30% balance can be exhausted through HEPA back into the room or to outside through a canopy unit (figure 3) 30% recirculated, 70% exhausted. Exhaust cabinet air must pass through a dedicated duct to the outside through a HEPA filter (figures 5A, 5B) No recirculation; total exhaust to the outside through a HEPA filter (figure 6) Similar to II, A1, but has 100 lfpm intake air velocity and plenums are under negative pressure to room; exhaust air can be ducted to outside through a canopy unit (figure 7) Supply air is HEPA filtered. Exhaust air passes through two HEPA filters in series and is exhausted to the outside via a hard connection (figure 8) Yes (minute amounts) Yes Yes Yes Yes Applications Volatile Toxic Chemicals and Radionuclides When exhausted outdoors 1,2 No Yes (minute amounts) 1,2 Yes (small amounts) 1,2 When exhausted outdoors (Formerly "B3") (minute amounts) 1,2 Yes (small amounts) 1,2 1. Installation may require a special duct to the outside, an in-line charcoal filter, and a spark proof (explosion proof) motor and other electrical components in the cabinet. Discharge of a Class I or Class II, Type A2 cabinet into a room should not occur if volatile chemicals are used. 2. In no instance should the chemical concentration approach the lower explosion limits of the compounds. 328
Appendix A Table 3. Field Performance Tests to be Applied to the Three Classes of Biological Safety Cabinets Biosafety Cabinet Test Performed for Class Class Class I II III Primary Containment Cabinet Integrity N/A A A (A1 Only) HEPA Filter Leak Req Req Req Downflow Velocity N/A Req N/A Face Velocity Req Req N/A Negative Pressure/Ventilation Rate B N/A Req Airflow Smoke Patterns Req Req E/F Alarms and Interlocks C,D C,D Req Electrical Safety Electrical Leakage, Etc. E,D E,D E,D Ground Fault Interrupter D D D Other Lighting Intensity E E E UV Intensity C,E C,E C,E Noise Level E E E Vibration E E E Req A B C D E F N/A Required during certification. Required for proper certification if the cabinet is new, has been moved or panels have been removed for maintenance. If used with gloves. If present. Encouraged for electrical safety. Optional, at the discretion of the user. Used to determine air distribution within cabinet for clean to dirty procedures. Not applicable. 329
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Biosafety in Microbiological and Bi
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SECTION VIII-H: Prion Diseases 299
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Forward Biosafety in Microbiologica
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Editors: L. Casey Chosewood, MD Dir
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Guest Editors: Matthew J. Arduino,
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Charles B. Millard, PhD Lieutenant
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Robert Bull Karen B. Byers Jane Cap
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Thomas L. Miller Douglas M. Moore M
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Robert Yarchoan Uri Yokel Lisa Youn
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Introduction laboratories chose not
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Introduction RISK CRITERIA FOR ESTA
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Introduction BMBL includes an impor
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Section II Biological Risk Assessme
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Biological Risk Assessment informat
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Biological Risk Assessment The NIH
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Biological Risk Assessment effectiv
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Biological Risk Assessment Third, m
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Biological Risk Assessment 14. Oxma
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Principles of Biosafety Safety equi
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Principles of Biosafety Often an in
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Principles of Biosafety Four standa
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Principles of Biosafety 4. Centers
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Laboratory Biosafety Level Criteria
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Section V Vertebrate Animal Biosafe
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Vertebrate Animal Biosafety Level C
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TABLE 1 SUMMARY OF RECOMMENDED BIOS
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Principles of Laboratory Biosecurit
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Occupational Health and Immunoproph
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Agent Summary Statements - Bacteria
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• Section VIII-B: Fungal Agents A
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Agent Summary Statements - Fungal A
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• Section VIII-C: Parasitic Agent
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Agent Summary Statements - Parasiti
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• Section VIII-D: Rickettsial Age
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Agent Summary Statements - Ricketts
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Agent Summary Statements - Ricketts
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• Section VIII-E: Viral Agents Ag
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Agent Summary Statements - Viral Ag
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Page 299 and 300: • Section VIII-H: Prion Diseases
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Page 307 and 308: Appendix A Proper maintenance of ca
Page 309 and 310: Appendix A declined. However, in ma
Page 311 and 312: Appendix A It is possible to exhaus
Page 313 and 314: Appendix A 4. The Class II, Type A2
Page 315 and 316: Appendix A prohibited. Furthermore,
Page 317 and 318: Appendix A plastic wrappers, pipett
Page 319 and 320: Appendix A touch-plate microburners
Page 321 and 322: Appendix A SECTION VI FACILITY AND
Page 323 and 324: Appendix A Development of Containme
Page 325 and 326: Appendix A C. Airflow Smoke Pattern
Page 327: Appendix A TABLES Table 1. Selectio
Page 331 and 332: Appendix A FIGURES Figure 1. HEPA f
Page 333 and 334: Appendix A Figure 3. The Class II,
Page 335 and 336: Appendix A Figure 5B. The Class II,
Page 337 and 338: Appendix A Figure 8. The Class III
Page 339 and 340: Appendix A Figure 9B. The vertical
Page 341 and 342: Appendix A Figure 11. A typical lay
Page 343 and 344: Appendix A REFERENCES 1. Centers fo
Page 345 and 346: Appendix B Decontamination and Disi
Page 347 and 348: Appendix B surfaces are contaminate
Page 349 and 350: Appendix B TABLE 1 DESCENDING ORDER
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Page 353 and 354: Appendix B d The effectiveness of a
Page 355 and 356: Appendix C Transportation of Infect
Page 357 and 358: Appendix C 1600 Clifton Road, N.E.,
Page 359 and 360: Appendix C disease in humans or in
Page 361 and 362: Appendix C Biological specimen, Cat
Page 363 and 364: Appendix D Agriculture Pathogen Bio
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Page 367 and 368: Appendix D 14. Pathological inciner
Page 369 and 370: Appendix D Wastes and other materia
Page 371 and 372: Appendix D Camelpox virus b a, b, c
Page 373 and 374: Appendix D SPECIAL ISSUES The impor
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Appendix D laboratory with enhancem
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Appendix D five day period of conta
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Appendix D slaughterhouses or indus
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Appendix D virus does not cause any
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Appendix D NDV is classified in the
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Appendix D movement permit is requi
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Appendix D Spring Viremia of Carp V
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Appendix D REFERENCES 1. Hess WR. A
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Appendix D 37. Alexander DJ. Newcas
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Appendix E Arthropod Containment Gu
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Appendix F Select Agents and Toxins
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Appendix G IPM issues and requireme
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Appendix H Working with Human, NHP
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Appendix I Guidelines for Work with
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decontaminated periodically, for ex
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0.25N, and/or sodium hypochlorite (
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k Irradiation causes a dose-depende
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7. Morin R, Kozlovac J. Biological
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Appendix J NIH Oversight of Researc
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Appendix K Resources for Informatio
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Appendix K E-mail: http://www2.gsu.
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Appendix K and http://www.who.int/c
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Appendix L HEPA High Efficiency Par
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