credits bmbl December 7 '06.doc - Central Michigan University

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Vertebrate Animal Biosafety Level Criteria – Animal Biosafety Level 4 1. The ABSL-4 suit laboratory consists of either a separate building or a clearly demarcated and isolated zone within a building. Laboratory doors must have locks in accordance with the institutional policies. Rooms in the facility must be arranged to ensure exit sequential passage through the chemical shower, inner (dirty) change room, personal shower, and outer (clean) changing area. Entry to this area must be through an airlock fitted with airtight doors. Personnel who enter this area must wear a positive pressure suit ventilated by a life support system with HEPA filtered breathing air. The breathing air system must have redundant compressors, failure alarms and emergency backup system. A chemical shower must be provided to decontaminate the surface of the positive pressure suit before the worker leaves the ABSL-4 laboratory. In the event of an emergency exit or failure of chemical shower, a method for decontaminating positive pressure suits, such as a gravity fed supply of chemical disinfectant, is needed. An automatically activated emergency power source must be provided at a minimum for the laboratory exhaust system, life support systems, alarms, lighting, entry and exit controls, BSCs, and door gaskets. Monitoring and control systems for air supply, exhaust, life support, alarms, entry and exit, and security systems should be on a UPS. A double-door autoclave, dunk tank, or fumigation chamber must be provided at the containment barrier for the passage of materials, supplies, or equipment. 2. Sinks inside the ABSL-4 laboratory should be placed near procedure areas and contain traps and be connected to the wastewater decontamination system. 3. Walls, floors, and ceilings of the ABSL-4 laboratory must be constructed to form a sealed internal shell to facilitate fumigation and prohibit animal and insect intrusion. The internal surfaces of this shell must be resistant to liquids and chemicals used for cleaning and decontamination of the area. Floors must be monolithic, sealed and coved. All penetrations in the internal shell of the laboratory, suit storage room and the inner change room must be sealed. Drains if present, in the laboratory floor must be connected directly to the liquid waste decontamination system. Sewer vents and other service lines must be protected by two HEPA filters in series and have protection against insect and animal intrusion. 114
Vertebrate Animal Biosafety Level Criteria – Animal Biosafety Level 4 Services, plumbing or otherwise that penetrate the laboratory walls, floors, ceiling, plumbing or otherwise, must ensure that no backflow from the laboratory occurs. These penetrations must be fitted with two (in series) backflow prevention devices. Consideration should be given to locating these devices outside of containment. Atmospheric venting systems must be provided with two HEPA filters in series and be sealed up to the second filter. Decontamination of the entire laboratory must be performed using a validated gaseous or vapor method when there have been significant changes in laboratory usage, before major renovations or maintenance shut downs, and in other situations, as determined by risk assessment. 4. Laboratory furniture must be of simple construction, capable of supporting anticipated loading and uses. Spaces between benches, cabinets, and equipment must be accessible for cleaning, decontamination and unencumbered movement of personnel. Chairs and other furniture should be covered with a non-porous material that can be easily decontaminated. Sharp edges and corners should be avoided. 5. Windows must be break-resistant and sealed. 6. BSCs and other primary containment barrier systems must be installed so that fluctuations of the room air supply and exhaust do not interfere with proper operations. BSCs should be located away from doors, heavily traveled laboratory areas, and other possible airflow disruptions. 7. <strong>Central</strong> vacuum systems are not recommended. If, however, there is a central vacuum system, it must not serve areas outside the ABSL-4 laboratory. Two in-line HEPA filters must be placed near each use point. Filters must be installed to permit in-place decontamination and replacement. 8. An eyewash station must be readily available in the laboratory area for use during maintenance and repair activities. 9. A dedicated non-recirculating ventilation system is provided. Only laboratories with the same HVAC requirements (i.e., other BSL-4 labs, ABSL-4, BSL-3 Ag labs) may share ventilation systems if each individual laboratory system is isolated by gas tight dampers and HEPA filters. The supply and exhaust components of the ventilation system must be designed to maintain the BSL-4/ABSL-4 laboratory at negative pressure to surrounding areas and provide differential pressure/directional airflow between adjacent areas within the laboratory. 115
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Biosafety in Microbiological and Bi
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SECTION VIII-H: Prion Diseases 299
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Forward Biosafety in Microbiologica
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Editors: L. Casey Chosewood, MD Dir
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Guest Editors: Matthew J. Arduino,
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Charles B. Millard, PhD Lieutenant
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Robert Bull Karen B. Byers Jane Cap
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Thomas L. Miller Douglas M. Moore M
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Robert Yarchoan Uri Yokel Lisa Youn
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Introduction laboratories chose not
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Introduction RISK CRITERIA FOR ESTA
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Introduction BMBL includes an impor
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Section II Biological Risk Assessme
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Biological Risk Assessment informat
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Biological Risk Assessment The NIH
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Biological Risk Assessment effectiv
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Biological Risk Assessment Third, m
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Biological Risk Assessment 14. Oxma
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Principles of Biosafety Safety equi
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Principles of Biosafety Often an in
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Principles of Biosafety Four standa
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Principles of Biosafety 4. Centers
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Laboratory Biosafety Level Criteria
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Page 63 and 64: Laboratory Biosafety Level Criteria
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Page 119 and 120: TABLE 1 SUMMARY OF RECOMMENDED BIOS
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Agent Summary Statements - Bacteria
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• Section VIII-B: Fungal Agents A
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Agent Summary Statements - Fungal A
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• Section VIII-C: Parasitic Agent
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Agent Summary Statements - Parasiti
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• Section VIII-D: Rickettsial Age
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Agent Summary Statements - Ricketts
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Agent Summary Statements - Ricketts
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• Section VIII-E: Viral Agents Ag
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Agent Summary Statements - Viral Ag
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Agent Summary Statements - Arboviru
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• Section VIII-G: Toxin Agents Se
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Agent Summary Statements - Toxins i
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Agent Summary Statements - Toxins A
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Agent Summary Statements - Toxins L
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Agent Summary Statements - Toxins e
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Agent Summary Statements - Toxins o
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Agent Summary Statements - Toxins t
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• Section VIII-H: Prion Diseases
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Agent Summary Statement - Prion Dis
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Appendix A Proper maintenance of ca
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Appendix A declined. However, in ma
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Appendix A It is possible to exhaus
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Appendix A 4. The Class II, Type A2
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Appendix A prohibited. Furthermore,
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Appendix A plastic wrappers, pipett
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Appendix A touch-plate microburners
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Appendix A SECTION VI FACILITY AND
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Appendix A Development of Containme
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Appendix A C. Airflow Smoke Pattern
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Appendix A TABLES Table 1. Selectio
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Appendix A Table 3. Field Performan
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Appendix A FIGURES Figure 1. HEPA f
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Appendix A Figure 3. The Class II,
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Appendix A Figure 5B. The Class II,
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Appendix A Figure 8. The Class III
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Appendix A Figure 9B. The vertical
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Appendix A Figure 11. A typical lay
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Appendix A REFERENCES 1. Centers fo
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Appendix B Decontamination and Disi
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Appendix B surfaces are contaminate
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Appendix B TABLE 1 DESCENDING ORDER
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Appendix B dioxide gas exits the ge
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Appendix B d The effectiveness of a
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Appendix C Transportation of Infect
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Appendix C 1600 Clifton Road, N.E.,
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Appendix C disease in humans or in
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Appendix C Biological specimen, Cat
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Appendix D Agriculture Pathogen Bio
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Appendix D simultaneous opening of
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Appendix D 14. Pathological inciner
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Appendix D Wastes and other materia
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Appendix D Camelpox virus b a, b, c
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Appendix D SPECIAL ISSUES The impor
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Appendix D required by the USDA and
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Appendix D depression, anorexia, an
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Appendix D laboratory with enhancem
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Appendix D five day period of conta
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Appendix D slaughterhouses or indus
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Appendix D virus does not cause any
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Appendix D NDV is classified in the
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Appendix D movement permit is requi
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Appendix D Spring Viremia of Carp V
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Appendix D REFERENCES 1. Hess WR. A
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Appendix D 37. Alexander DJ. Newcas
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Appendix E Arthropod Containment Gu
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Appendix F Select Agents and Toxins
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Appendix G IPM issues and requireme
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Appendix H Working with Human, NHP
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Appendix I Guidelines for Work with
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decontaminated periodically, for ex
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0.25N, and/or sodium hypochlorite (
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k Irradiation causes a dose-depende
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7. Morin R, Kozlovac J. Biological
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Appendix J NIH Oversight of Researc
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Appendix K Resources for Informatio
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Appendix K E-mail: http://www2.gsu.
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Appendix K and http://www.who.int/c
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Appendix L HEPA High Efficiency Par
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