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Saddleback Journal of Biology - Saddleback College

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Fall 2009 <strong>Biology</strong> 3B Paper<br />

Mean Colonies <strong>of</strong> E.Coli After Exposure<br />

200<br />

180<br />

160<br />

140<br />

120<br />

100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

E. Coli H.W.B.<br />

E. Coli Irradiated<br />

y = 142.2e -0.032x<br />

R² = 0.9905<br />

y = 211.11e -0.104x<br />

R² = 0.965<br />

0 50 100<br />

Time (sec)<br />

FIGURE 1.Mean number <strong>of</strong> colonies relative to time in seconds exposed. Showing exponential trend lines for 100°C<br />

Hot Water Bath (H.W.B.) and Microwave Irradiation at 2,450MHz (M.I.)<br />

160<br />

mean colonies <strong>of</strong> E. coli produced after<br />

exposure<br />

140<br />

120<br />

100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

Control 30s HWB 60s HWB 30s MI 60s MI<br />

interval and exposure type<br />

FIGURE 2.Bar graph <strong>of</strong> mean colonies <strong>of</strong> E. coli produced after exposures followed by 96 hour incubation at 37°C.<br />

Error bars are mean±SEM. HWB=Hot Water Bath at 100°C, MI= Microwave Irradiated at 2,450MHz. No<br />

significant difference p=0.0125.<br />

146<br />

<strong>Saddleback</strong> <strong>Journal</strong> <strong>of</strong> <strong>Biology</strong><br />

Spring 2010

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