Saddleback Journal of Biology - Saddleback College

More documents

Recommendations

Info

$Math 10 â Statistics - Saddleback College$

hemolymph concentrations were hyperosmotic to the media in which the crabs were kept and significantly different from each other. Brown and Terwilliger (1992) carried out a similar study on Dungeness crabs (Cancer magister) and obtained the same results. Susanto and Charmantier (1999) worked with a freshwater crustacean -- crayfish Astacus leptodactus; their experiment demonstrated that hemolymph concentarions of A. leptodactus were hyperosmotic to experimentally increased salinities of water up to 13‰ salinity and isoosmotic at higher salinities up to 21‰. The present study examined osmoregulation in green shore crab (Hemigrapsus oregonensis), which inhabit intertidal zone, at three different salinities of water: approximately 33‰, 20‰, and 10‰. It was hypothesized that at three different salinities hemolymph osmolalities of H. oregonensis would be hyperosmotic to the surrounding medium and significantly different from each other. Materials and Methods H. oregonensis were collected at Doheny State Beach, Dana Point, CA. In the laboratory the crabs were divided between two glass containers with water about 6 cm deep; the containers were set into an aquarium with gravel. Over the course of the experiment, the water in each container was aerated and kept at room temperature (20° C). First, H. oregonensis were placed into 33‰ salinity water. Crabs were allowed to acclimate for 24 hours; after that samples of hemolymph were collected from each crab. The same procedure was repeated after transferring the crabs into water of approximately 20‰ salinity, followed by transferring the crabs into Hemolymph Osmolality (mmol/kg) 1200 1000 800 600 400 200 Spring 2010 Biology 3B Paper 0 water of approximately 10‰ salinity. Thus, 3 rounds of hemolymph samples at three different salinities of water were collected. A 27 gauge syringe was used to collect hemolymph. The needle of a syringe was inserted through the membrane of the last walking leg, and 10 µL of hemolymph were removed. Osmolality of hemolymph samples was measured with a Wescor vapor pressure osmometer. Desired salinities of water were achieved by diluting 33‰ salinity water with distilled water in the appropriate proportion. A sample of water of each salinity was taken after 24-hour acclimation period, and osmolality of water was measured. Upon completion of the experiment the crabs were released back to their natural habitat. Results The mean osmolality of water of approximately 33‰ salinity was 1058 mmol/kg ± 13mmol/kg (±SEM, N=2); the mean osmolality of hemolymph of H. oregonensis acclimated to this salinity was 955 mmol/kg ± 14.3 mmol/kg (±SEM, N=8), which was hypoosmotic to the surrounding medium. The mean osmolalities of water of approximately 20‰ and 10‰ salinities were 645 mmol/kg ± 1 mmol/kg (±SEM, N=2) and 312 mmol/kg ± 8 mmol/kg (±SEM, N=2) respectively; the mean osmolalities of hemolymph of the crabs acclimated to these salinities were 882 mmol/kg ± 9.20 mmol/kg (±SEM,N=11) in 645 mmol/kg water osmolality and 724 mmol/kg ± 57.3mmol/kg (±SEM, N=10) in 312 mmol/kg water osmolality, both of which were hyperosmotic to the surrounding medium(Figure 1) . 1058 645 312 Water Osmolality (mmol/kg) Figure 1. The mean osmolalities of hemolymph of H. oregonensis acclimated to three different water osmolalities. Error bars indicate standard errors of the mean. 13 Saddleback Journal of Biology Spring 2010
Spring 2010 Biology 3B Paper ANOVA indicated that the mean hemolymph osmolalities of H. oregonensis acclimated to three different salinities of water were significantly different from each other (p = 4.2×10 -4 ). PostHoc analysis showed the following: there was no significant difference between the mean hemolymph osmolalities of H. oregonensis acclimated to 1058 mmol/kg water osmolality and the crabs acclimated to 645 mmol/kg water osmolality (p>0.05); there was a significant difference between mean hemolymph 1200 osmolalities of the crabs acclimated to 645 mmol/kg water osmolality and the crabs acclimated to 312 mmol/kg water osmolality (p
Page 1 and 2: Saddleback Journal of Biology Stoma
Page 3 and 4: TABLE OF CONTENTS Peer Reviewed Man
Page 5 and 6: Author(s) Title Page Angela C. Park
Page 7 and 8: TABLE OF CONTENTS Biology 3A Abstra
Page 9 and 10: Spring 2010 Biology 3B Paper Hills,
Page 11 and 12: Spring 2010 Biology 3B Paper Herppi
Page 13 and 14: Spring 2010 Biology 3B Paper 20 18
Page 15 and 16: Spring 2010 Biology 3B Paper patter
Page 17 and 18: Spring 2010 Biology 3B Paper Number
Page 19: Spring 2010 Biology 3B Paper Dimorp
Page 23 and 24: Spring 2010 Biology 3B Paper throug
Page 25 and 26: Spring 2010 Biology 3B Paper Table
Page 27 and 28: Spring 2010 Biology 3B Paper which
Page 29 and 30: Spring 2010 Biology 3B Paper Garlic
Page 31 and 32: Spring 2010 Biology 3B Paper Table
Page 33 and 34: Spring 2010 Biology 3B Paper Figure
Page 35 and 36: Spring 2010 Biology 3B Paper These
Page 37 and 38: Spring 2010 Biology 3B Paper distri
Page 39 and 40: Spring 2010 Biology 3B Paper Mycoba
Page 41 and 42: Spring 2010 Biology 3B Paper G ro w
Page 43 and 44: Spring 2010 Biology 3B Paper superv
Page 45 and 46: Spring 2010 Biology 3B Paper Studie
Page 47 and 48: Spring 2010 Biology 3B Paper The Ef
Page 49 and 50: Spring 2010 Biology 3B Paper 50 Mea
Page 51 and 52: Spring 2010 Biology 3B Paper Blood
Page 53 and 54: Spring 2010 Biology 3B Paper The fo
Page 55 and 56: Spring 2010 Biology 3B Paper Introd
Page 57 and 58: Spring 2010 Biology 3B Paper The da
Page 59 and 60: Spring 2010 Biology 3B Paper Materi
Page 61 and 62: Spring 2010 Biology 3B Paper Method
Page 63 and 64: Spring 2010 Biology 3B Paper Discus
Page 65 and 66: Fall 2009 Biology 3B Paper signific
Page 67 and 68: Fall 2009 Biology 3B Paper Mean Cha
Page 69 and 70: Fall 2009 Biology 3B Paper Mean Cha
Page 71 and 72:
Fall 2009 Biology 3B Paper Velasque
Page 73 and 74:
Fall 2009 Biology 3B Paper (192 mM
Page 75 and 76:
Fall 2009 Biology 3B Paper 0.9 0.8
Page 77 and 78:
Fall 2009 Biology 3B Paper immunobl
Page 79 and 80:
Fall 2009 Biology 3B Paper integrif
Page 81 and 82:
Fall 2009 Biology 3B Paper This cou
Page 83 and 84:
Fall 2009 Biology 3B Paper arterial
Page 85 and 86:
Fall 2009 Biology 3B Paper to five
Page 87 and 88:
Fall 2009 Biology 3B Paper The Effe
Page 89 and 90:
Fall 2009 Biology 3B Paper Length o
Page 91 and 92:
Fall 2009 Biology 3B Paper In order
Page 93 and 94:
Fall 2009 Biology 3B Paper The meas
Page 95 and 96:
Fall 2009 Biology 3B Paper Introduc
Page 97 and 98:
Fall 2009 Biology 3B Paper 4.4 Chan
Page 99 and 100:
Fall 2009 Biology 3B Paper percent
Page 101 and 102:
Fall 2009 Biology 3B Paper another,
Page 103 and 104:
Fall 2009 Biology 3B Paper Figure 1
Page 105 and 106:
Fall 2009 Biology 3B Paper Red-Wing
Page 107 and 108:
Fall 2009 Biology 3B Paper Cromie,
Page 109 and 110:
Fall 2009 Biology 3B Paper Discussi
Page 111 and 112:
Fall 2009 Biology 3B Paper up for 1
Page 113 and 114:
Page 115 and 116:
Fall 2009 Biology 3B Paper metaboli
Page 117 and 118:
Fall 2009 Biology 3B Paper conducte
Page 119 and 120:
Fall 2009 Biology 3B Paper College
Page 121 and 122:
Fall 2009 Biology 3B Paper Results
Page 123 and 124:
Fall 2009 Biology 3B Paper Roneus,
Page 125 and 126:
Fall 2009 Biology 3B Paper Results
Page 127 and 128:
Page 129 and 130:
Fall 2009 Biology 3B Paper nature o
Page 131 and 132:
Fall 2009 Biology 3B Paper the part
Page 133 and 134:
Fall 2009 Biology 3B Paper Korol, D
Page 135 and 136:
Fall 2009 Biology 3B Paper is so in
Page 137 and 138:
Fall 2009 Biology 3B Paper 12 10 Di
Page 139 and 140:
Fall 2009 Biology 3B Paper Water Qu
Page 141 and 142:
Fall 2009 Biology 3B Paper Mean MPN
Page 143 and 144:
Fall 2009 Biology 3B Paper ones. Wi
Page 145 and 146:
Fall 2009 Biology 3B Paper Schroete
Page 147 and 148:
Fall 2009 Biology 3B Paper 16 14 12
Page 149 and 150:
Fall 2009 Biology 3B Paper consumer
Page 151 and 152:
Fall 2009 Biology 3B Paper The smal
Page 153 and 154:
Fall 2009 Biology 3B Paper Mean Col
Page 155 and 156:
Fall 2009 Biology 3B Paper Introduc
Page 157 and 158:
Fall 2009 Biology 3B Paper Five sam
Page 159 and 160:
Spring 2010 Biology 3A Abstracts 4.
Page 161 and 162:
Spring 2010 Biology 3A Abstracts 10
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Fall 2009 Biology 3A Abstracts 4. A
Page 169 and 170:
Fall 2009 Biology 3A Abstracts 8. T
Page 171 and 172:
Fall 2009 Biology 3A Abstracts 12.
Page 173 and 174:
Fall 2009 Biology 3A Abstracts 18.
show all

Saddleback Journal of Biology - Saddleback College

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?