Saddleback Journal of Biology - Saddleback College

Fall 2009 Biology 3B Paper
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Figure 7. CTR1 protein detection and expression through western blotting reveals that there is increased CTR1
protein expression in mouse mammary explants when treated with lactational hormonal, compared to non-hormonal
treatment in two mice. CTR1 was once again detected by bands at the 105 kDa mark, where the trimer should exist
at. This replication saw obvious increase in CTR1 expression with hormonal treatment.
Effect of Lactational Hormones on Ceruloplasmin Protein Expression
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Lane Sample
1 1:10 dilution of mouse plasma as control
2 Mouse A 48 hour treatment without hormone
3 Mouse A 48 hour treatment with hormone
4 Mouse B 48 hour treatment without hormone
5 Mouse B 48 hour treatment with hormone
6 Mouse A 96 hour treatment without hormone
7 Mouse A 96 hour treatment with hormone
8 Mouse B 96 hour treatment without hormone
9 Mouse B 96 hour treatment with hormone
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Figure 8. Western blot for ceruloplasmin in mouse mammary explant secretions. The desired band size of 130 kDa
was not present. Non-specific bands (probably albumin) were detected after long exposure to substrate.
Discussion
Our findings have shown that there was no
statistically significant difference in the expression of
CTR1 mRNA treated with siCTR1 compared with the
nonspecific siRNA, indicating no CTR1 knockdown.
Likewise, there was no significant difference in the
amount of CTR1 protein expression based on our
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Saddleback Journal of Biology
Spring 2010