Saddleback Journal of Biology - Saddleback College

Fall 2009 Biology 3B Paper
300000
250000
200000
CPM
150000
siCTR
siNEG
100000
50000
0
Apical
Cellular
Figure 3 Effect of CTR1 Knockdown: Copper uptake by PMC42 cell monolayers, in cells where CTR1 mRNA
knockdown was successful (comparing siRNA negative cells - purple; with CTR1 siRNA treated cells - blue). No
differences in uptake of 64Cu into cells or transfer to the apical medium were observed.
Effect of Lactational Hormones on CTR1 mRNA and Protein Expression
mRNA expression of CTR1
Real Time PCR was performed on mouse mammary explants in order to observe the mRNA expression of CTR1.
The amount of mRNA present in samples treated with and without lactational hormones allowed us to determine if
CTR1 responded to the treatment. There was no statistical difference in CTR1 mRNA expression when treated with
hormones having 1.47 million copies versus non-hormonal treatment having 0.66 million copies (figure 4). A
decrease in CTR1 expression at the mRNA was observed in one case, opposite of our expected results (figure 5).
Effect of lactational hormones on CTR-1 in mouse mammary explants
2.5
2.0
Relative CTR-1 mRNA
1.5
1.0
0.5
0.0
Non-Hormone
Hormone
Figure 4 Effect of lactational hormones on CTR1 mRNA expression in mouse mammary explants based upon data
from Real Time -PCR. The results did not show any statistical difference in CTR1 mRNA expression in the presence
of lactational hormones.
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Saddleback Journal of Biology
Spring 2010