14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 77.006 Session: Gram-positives & Miscellaneous Pathogens Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation The detection of antibodies directed against specific antigens of Borrelia burgdorferi sensu lato (B. burgdorferi s.s., B. afzelii, B. garinii, B. spielmanii) in patients with borreliosis in Eastern Poland M. Tokarska-Rodak 1 , H. Fota-Markowska 1 , M. Kozio-Montewka 1 , F. miechowicz 1 , B. Gajownik 2 , M. Prokop 3 , R. Modrzewska 1 1 Medical University, Lublin, Poland, 2 Public Hospital, uków, Poland, 3 Military Hospital, Lublin, Poland Background: The most common Borrelia genospecies involved in the etiology of Lyme disease in Eastern Europe are: B. burgdorferi s.s., B. garinii, and B. afzelii. However, the role of other genospecies including B. spielmanii has also been suggested. In view of a paucity of epidemiologic data concerning the role of B. spielmanii as the potential causative agent of Lyme disease in Poland, investigation towards identification of patients with serologic evidence of infection caused by this Borrelia genospecies was undertaken. Methods: The study group comprised 41 patients from Eastern Poland with borreliosis (age range: 19-65 yrs). Detection of IgG and IgM antibodies to B. burgdorferi s.l. was performed using test (Mikrogen) which included the following antigens for IgM and IgG: OspC and p18 (B. burgdorferi s.s., B. afzelii, B. garinii, B. spielmanii), VlsE, p100, p58, p41, p39, and OspA. Results: The results of the study indicate that the simultaneous presence of IgM antibodies against OspC protein characteristic of all four genospecies (B. burgdorferi s.s., B. afzelii, B. garinii, and B. spielmanii) was detected in 36 patients with clinical manifestations of borreliosis. The presence of IgM antibodies to OspC antigen of B. spielmanii accompanied by negative results for the presence of antibodies against antigens of the remaining genospecies was detected in one patient. The IgG antibodies against B. burgdorferi s.l. were observed with varying frequency: - anti- VlsE in 22 patients, - anti-OspC for four genospecies: B. burgdorferi s.s., B. afzelii, B. garinii and B. spielmanii in 9 patients, - anti-p18 for single genospecies, more often for B. afzelii (4 patients) and B. spielmanii (5 patients). Conclusion: The results of the study suggest the possibility of co-infections with several genospecies of B. burgdorferi s.l. evidenced by the presence of IgM antibodies to OspC of B. burgdorferi s.s., B. afzelii, B. garinii, and B. spielmanii. The detection of antibodies directed against specific B. spielmanii antigens suggests that this microorganism may be responsible for triggering borreliosis both as a single etiologic agent and in co-infection with other Borrelia genospecies.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 77.007 Session: Gram-positives & Miscellaneous Pathogens Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Enterococcal bone and joint infections: A working group experience along 17 years C. A. Pensotti 1 , F. Nacinovich 2 , C. Di Stéfano 3 , J. Thierer 4 , A. Ferraris 5 , D. Stamboulian 6 1 FUNCEI and Clinica Privada Monte Grande, Ciudad Autonoma de Buenos Aires, Argentina, 2 FUNCEI and ICBA, Buenos Aires, Argentina, 3 FUNCEI , Ciudad Autonoma de Buenos Aires, Argentina, 4 ICBA, Ciudad Autonoma de Buenos Aires, Argentina, 5 FUNCEI, Ciudad Autónoma de Buenos Aires, Argentina, 6 Clinical Director of FUNCEI, Ciudad Autonoma de Buenos Aires, Argentina Background: Enterococcal bone and joint infections (EBJI) are an uncommon disease and the optimal treatment and outcome have been poorly defined. The objective was to analize the outcome of EBJI episodes (ep.) according to patients’ characteristics and microbiological features, considering type of surgery implemented and antibiotic treatment chosen. Methods: We performed a retrospective cohort study of patients with EBJI, treated by our working group from 1/91 to 3/09. Categorical variables are presented as percentages and continuous variables as median and interquartile range (IQR). Differences were considered statistically significant at p
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