14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 79.010
Session: Obstetrical-Gynecological, Surgical and Sexually Transmitted Infections
Date: Friday, March 12, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Superoxide anion in lesions of cervix associated or not papillomavirus-infection
Y. Carrero, D. Callejas, R. Mindiola, L. Porto, F. Alana, M. E. castellanos, C. Silva, L. Costa
Universidad del Zulia, Maracaibo, Zulia, Venezuela
Objective: To determine the expression of superoxide anions in premalignant and malignant
lesions of the cervix associated or not with human papillomavirus (HPV).
Methods: A total of 74 patients (45 CINI, 5 CIN II, 5 CIN III and 5 patients with Cancer) 7 women
who had inflammation and 7 apparently healthy women as controls, were sampled for cytology,
cervical exudate HPV and 2 biopsies determine: 1) fixed in 10% formalin for histological analysis,
2) component in OCT (Tissue Tek) to determine the expression of superoxide anions by reaction
of Briggs, one carries out the identification and genotyping of HPV Hybrid Capture (HC2).
Results: Of the patients studied 23% were positive for HPV infection with a 58.5% high-risk
genotypes were significant differences in the number of superoxide anion positive cells in both
epithelium and stroma in different degrees of damage observed increased as the degree of lesion
progressed
Conclusion: The results allow the inference that the superoxide anion plays an important role in
the genesis and progression of lesions in the cervix, but can not be used as markers of tumor
progression.