14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 82.013 Session: Trypanosomiasis, Leishmaniasis & Schistosomiasis Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Chagas disease: Mother to child transmission (MTCT). A single experience in a public hospital from Buenos Aires M. T. Rodriguez Brieschke 1 , M. Seoane 1 , C. Nieto 1 , V. Loggia 1 , E. Bottaro 2 , M. Giacco 1 , J. C. Gonzalez 1 , P. G. Scapellato 1 1 Hospital D F Santojanni, Buenos Aires, Argentina, 2 Hospital General de Agudos D F Santojanni, Buenos Aires, Argentina Background: Mother to child transmission of Chagas Diseases (ChD) rates was reported from 1 to > 10%. Factors reported to increase risk include younger maternal age, human immunodeficiency virus infection, and parasite strain. Methods: Retrospective analysis of children assisted in infectious diseases department from 2001 to 2008. Inclusion criteria was: every child born from a mother suffering of ChD with accesing a diagnostic procedures before 24 months of life and never traveled to endemics areas until finish diagnostic process. Diagnosis of ChD among mothers and children over 6 months old were performed by serology (ELISA and HAI). Diagnosis of ChD among children 6 months was performed with parasitemia investigation. Results: We studied 307children born from mother with ChD, 165 were 24 moths of life. Ratio man/woman 87/78. MTCT was 26/152 (17%). Diagnosis was performed by parasitemia in 20/26: the 1st parasitemia perfomed was positive in 13 cases, 2nd parasitemia was positive in 5 cases and 3rd parsitemia was positive in only a case. In six patients, ChD diagnosis was performed by serology. Mothers were from: Argentina=28, Bolivia=96, Paraguay=19 and without records=22. Only 11/26 chagasic children were symptomatic. Most frequent symptoms were: anemia 9/11, hepatosplenomegaly 3/11, hydrops fetalis 1/11, sepsis 1/11, abnormalities in serum transaminases 1/11 and cardiac abnormalities 1/11. Prematurity was observing in 5/26 with ChD vs 2/111 no ChD. Low birth weight was present in 5/26 (19.2%) with ChD vs 6/111(5.4%) no ChD. Three patients born from mother with HIV infection. MTCT were 3/3 (100%) vs 23/145 (15.8%) no HIV. No children were infected with HIV. The study of newborn allowed us performed study of ChD among 97 sibblings never studied; in 11 of them ChD was diagnosed. Benzindazol was indicated in all cases of vertically acquired ChD; 9 of the were lost of follow up and 5 had adverse reactions that. Conclusion: MTCT was higher than previously reported. Even if sample was small; it can be possible that mothers with HIV co-infection have more MTCT than no HIV. We have the opportunity to study all the members of family and bring treatment if it is necessary.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 82.014 Session: Trypanosomiasis, Leishmaniasis & Schistosomiasis Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Trypanosoma cruzi expressing luciferase for drug screening using a bolivian strain S. Nunez 1 , H. Mayta 2 , M. Verastegui 3 , C. Lafuente 4 , C. Bern 5 , R. Gilman 6 1 Prisma, Lima 31, Peru, 2 Universidad Peruana Cayetano Heredia, Lima 31, Peru, 3 Universidad Peruana Cayetano Heredia, Lima, Peru, 4 Hospital Universitario Japonés, , Santa Cruz, Bolivia, 5 CDC, Atlanta, AT, USA, 6 Johns Hopkins University, Baltimore, MD, USA Background: The commonly used drugs against T. cruzi are nifurtimox and benznidazole but they both have adverse effects for humans. Clinical symptoms of Chagas disease also depends on the strain present in each region. Drug screening methods are tedious and expensive. Highthroughput systems are required for screening anti-T. cruzi agents. The use of transfected parasites expressing reporter genes like -galactosidase has facilitated drug screening. However, luciferase (luc) has a higher sensibility. In our studies we found clinical and serological differences between the Peruvian and Bolivian strain. The Bolivian strain seems more virulent as it has been documented with a high number of acute and chronic cases. Here we developed transgenic parasites expressing luciferase gene for having a new drug screening method. Methods: Bolivian strain (DH29) was isolated from a child who acquired Chagas Disease by congenital infection based on microscopic examination and parasites were recovered by in vitro cultivation. The open reading frame of luc was inserted in two vectors: pBS:CL-Neo-01/BC-LacZ- 10 (pLAC) and pTREX. Stable transfectans were isolated with G418 following integration of the circular transforming plasmids into the nucleus of epimastigotes by electroporation. Luciferase was detected by PCR, western blot and its activity with a luminometer. Results: Two plasmids were developed pBS-Neo-Luc and pR-Luc-Neo. Drug (G418)-resistant parasites were isolated after three weeks post electroporation. PCR (1.7Kb) and western blot (61 KD) detected luciferase gene. Transfected parasites incubated with luciferin showed luciferase activity. The clones that failed to express luciferase were found to have integrated the neomicin gene. The clones that expressed luciferase were stable for more than six months without selection. Two clones (L95 y L97) expressing luciferase were isolated from DH29 strain. There is a correlation between the enzyme activity and number of parasites. Luciferase assay was able to detect 102 parasites ml-1. Conclusion: The constructed plasmids pBS-Neo-Luc and el pR-Luc-Neo are useful for stable expression in T. cruzi. Two clones L95 and L97 from DH29 strain of T. cruzi expressing luciferase gene were developed.There is a linear correlation between luciferase activity and the number of parasites ml-1. Luciferase assay is able to detect at least 102 parásitos ml-1.
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