14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 75.030
Session: Diagnostics
Date: Friday, March 12, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Production and standardization of Brucella national standard antiserum equivalent to OIEISS in
Sudan
M. F. E. M. Alawad 1 , M. T. Musa 2
1 Animal Resources Research corporation, Central vet.Research lab.(CVRL) -Soba, Khartoum,
Sudan, 2 Animal Resources Research Corporation, Khartoum, Sudan
Background: Brucellosis is a bacterial contagious zoonotic disease caused by one of the ninth
species of the genus Brucella, including the three proposed new species Brucella
ceti,B.pinnipedis (Foster et al.,2002&2007) and B.microti (Scholz et.al, 2008a; 2008b).
The serological tests were rapid and applicable methods for Brucellosis diagnosis using validated
test methods and standardized reagents.
For the control of brucellosis at the national or local level, the buffered Brucella antigen tests
(Boats), i.e. the Rose Bengal test (RBT) and the buffered plate agglutination test (BPAT), as well
as the ELISA and the FPA, are suitable screening tests. Positive reactions should be retested
using a suitable confirmatory and/or complementary strategy (OIE manual, 2009).
The OIE reference standards are those against which all other standards are compared and
calibrated. These reference standards are all available to national reference laboratories and
should be used to establish secondary or national standards against which working standards
can be prepared and used in the diagnostic laboratory for daily routine use. These sera have
been developed and designated by the OIE as International Standard Sera. The use of these
promotes international harmonisation of diagnostic testing and antigen standardisation.
Methods: Sudanese national standard antiserum equivalent to OIEISS was produced and
standardised from naturally infected cows with a high titre of Brucella according to Veterinary
laboratory agency-Webridge Laboratory methods (Hendry etal., 1985) and OIE manual (OIE,
2009). Apanel of naturally infected cows sera were tested for Brucella Agglutinins by RBT, SAT
and ELISA.The selected sera and OIEISS were titrated, tested and compared with previously
mentioned serological tests.
Results: An sterile pooled high titre sera were successfully prepared. The serum was diluted
perfectly with negative serum to match with the OIEISS titre which was 1000 IU.
Conclusion: This study was result in production of standardized National standard antiserum for
the first time in Sudan, when checked locally using the appropriate methods of Veterinary
laboratory agency-Webridge, but needs further quality control check in the specialist OIE
reference laboratories.