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14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference:<br />

Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.<br />

Please note that the official publication of the <strong>International</strong> Journal of <strong>Infectious</strong> Diseases 2010, Volume 14, Supplement 1<br />

is available electronically on http://www.sciencedirect.com<br />

Final Abstract Number: 80.026<br />

Session: Pediatric and Perinatal Infections<br />

Date: Friday, March 12, 2010<br />

Time: 12:30-13:30<br />

Room: <strong>Poster</strong> & Exhibition Area/Ground Level<br />

Type: <strong>Poster</strong> Presentation<br />

Detection of rotavirus antigen in stools samples collected from children in parts of Nigeria<br />

D. Ehichioya 1 , C. Bode 2 , C. J. Elikwu 3 , I. Ossai 4 , R. Orenolu 5 , S. Omilabu 6<br />

1 University of Lagos, Lagos, Nigeria, 2 University Teaching Hospital, Lagos, Nigeria, 3 Lagos<br />

University Teaching Hospital, Idi-araba, Nigeria, Lagos, Nigeria, 4 University of Benin Teaching<br />

Hospital,, Benin, Edo, Nigeria, 5 College of Medicine, University of Lagos, Idi-araba, P.M.B.<br />

12003, Lagos,, Lagos, Nigeria, 6 College of Medicine, University of Lagos, Idi-araba, P.M.B.<br />

12003, Lagos, lagos, Nigeria<br />

Background: Rotavirus is the most common cause of severe diarrhoeal disease in infants and<br />

young children all over the world. About 40% of more than 125 million cases of diarrhoea each<br />

year in the world are attributed to rotavirus. According to reports of the Center <strong>for</strong> Disease Control<br />

and Prevention (CDC) in the United States, each year, rotavirus causes approximately 111 million<br />

infections, 25 million hospitalizations, and 440,000 deaths in children less than five years of age,<br />

worldwide, and the total associated medical costs due to rotavirus infection are estimated to be<br />

enormous.<br />

In Nigeria, about three hundred children under the age of five are thought to be lost annually to<br />

diarrhoea.<br />

Methods: Data including stool samples and background in<strong>for</strong>mation were collected from 157<br />

diarrhoeic children aged less than five years; from eight different hospitals in the southern part of<br />

Nigeria between June, 2006 and July 2009. Viral RNA was extracted from the stool samples with<br />

the RNA mini-kit (Qiagen) and a fragment of the VP7 gene was amplified with the Reverse<br />

transcriptase polymerase chain reaction (RT-PCR), from the extracted RNA.<br />

Results:<br />

Of the 157 specimens collected from these hospitals, Rotavirus was amplified from 29 (18.5%) of<br />

them. There was no significant sex preponderance observed (p

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