14th ICID - Poster Abstracts - International Society for Infectious ...
14th ICID - Poster Abstracts - International Society for Infectious ...
14th ICID - Poster Abstracts - International Society for Infectious ...
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When citing these abstracts please use the following reference:<br />
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.<br />
Please note that the official publication of the <strong>International</strong> Journal of <strong>Infectious</strong> Diseases 2010, Volume 14, Supplement 1<br />
is available electronically on http://www.sciencedirect.com<br />
Final Abstract Number: 80.026<br />
Session: Pediatric and Perinatal Infections<br />
Date: Friday, March 12, 2010<br />
Time: 12:30-13:30<br />
Room: <strong>Poster</strong> & Exhibition Area/Ground Level<br />
Type: <strong>Poster</strong> Presentation<br />
Detection of rotavirus antigen in stools samples collected from children in parts of Nigeria<br />
D. Ehichioya 1 , C. Bode 2 , C. J. Elikwu 3 , I. Ossai 4 , R. Orenolu 5 , S. Omilabu 6<br />
1 University of Lagos, Lagos, Nigeria, 2 University Teaching Hospital, Lagos, Nigeria, 3 Lagos<br />
University Teaching Hospital, Idi-araba, Nigeria, Lagos, Nigeria, 4 University of Benin Teaching<br />
Hospital,, Benin, Edo, Nigeria, 5 College of Medicine, University of Lagos, Idi-araba, P.M.B.<br />
12003, Lagos,, Lagos, Nigeria, 6 College of Medicine, University of Lagos, Idi-araba, P.M.B.<br />
12003, Lagos, lagos, Nigeria<br />
Background: Rotavirus is the most common cause of severe diarrhoeal disease in infants and<br />
young children all over the world. About 40% of more than 125 million cases of diarrhoea each<br />
year in the world are attributed to rotavirus. According to reports of the Center <strong>for</strong> Disease Control<br />
and Prevention (CDC) in the United States, each year, rotavirus causes approximately 111 million<br />
infections, 25 million hospitalizations, and 440,000 deaths in children less than five years of age,<br />
worldwide, and the total associated medical costs due to rotavirus infection are estimated to be<br />
enormous.<br />
In Nigeria, about three hundred children under the age of five are thought to be lost annually to<br />
diarrhoea.<br />
Methods: Data including stool samples and background in<strong>for</strong>mation were collected from 157<br />
diarrhoeic children aged less than five years; from eight different hospitals in the southern part of<br />
Nigeria between June, 2006 and July 2009. Viral RNA was extracted from the stool samples with<br />
the RNA mini-kit (Qiagen) and a fragment of the VP7 gene was amplified with the Reverse<br />
transcriptase polymerase chain reaction (RT-PCR), from the extracted RNA.<br />
Results:<br />
Of the 157 specimens collected from these hospitals, Rotavirus was amplified from 29 (18.5%) of<br />
them. There was no significant sex preponderance observed (p