14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 84.042 Session: Virology and Viral Infections (Non-HIV) Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Seroprevalence of human cytomegalovirus infection in Singapore H. N. Leong, B. H. Tan, S. H. Lim, K. P. Chan Singapore General Hospital, Singapore, Singapore Background: The human cytomegalovirus virus (HCMV) is a ubiquitous betaherpesvirus, common in all human populations, with seroprevalence of 80-100% in adults across the world. Most infections are harmless, but it can cause congenital HCMV infection in neonates or disseminated disease in the immunocompromised hosts. Till date, there has been scarce seroprevalence data of this disease in our country. Methods: A retrospective analysis of the HCMV IgG assays performed in our laboratory was done. The period of study was from 2004 to 2007. Results: A total of 3582 patient data were available. Duplicates and patient with missing variables (age, sex) were removed, leaving 2042 patients (median of 707 patients per year) for analysis. Across the 4 years of data, there was no significant difference seen in across the age groups, sex or race. An overall seropositivity rate was 40% for the 1-10 year age group. This rises gradually to 44%, 73%, 82%, 90%, 97% and 100% for each decade increase. The males and females have a similar rate of acquisition of HCMV seropositivity at age group 1-10 of 40%. However, seropositivity in females increases rapidly compared to the males in the age groups 10- 20 to 40-50 before being similar again in the older age groups. This difference becomes statistically different in the 20-30 year age group (p=0.0024). Amongst the races, the predominant Chinese race group has a lower seropositivity in all age groups from 1-10 to 40-50 compared to the minority races of Indians and Malays combined. This difference was statistically different in the 20-30 year age group (p=0.001). The differences are likely related to the social habits of each race, and sex. Conclusion: The result show a HCMV seroprevalence of 73% in the adult age group of 20-30. This prevalence is higher for females and in the minority races. This data has implications in estimating the risk for congenital CMV disease in neonates born to mothers acquiring their primary infection during pregnancy, and risk of CMV disease in transplant programmes.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 84.043 Session: Virology and Viral Infections (Non-HIV) Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Viral load and genome integration detection: Two molecular markers for HPV persistent infection C. Jurgensen 1 , S. Vielma 1 , E. Michelli 1 , L. Tellez 1 , J. Mendoza 1 , M. Muñoz 1 , M. Correnti 2 , M. Cavazza 3 1 Universidad de Los Andes, Facultad de Medicina, Merida, Merida, Venezuela, 2 Universidad Central de Venezuela, Caracas, Distrito Capital, Venezuela, 3 Universidad de Los Andes, Caracas, Distrito Capital, Venezuela Background: Detection and quantification of Human Papillomavirus (HPV) are predicting factors associated with cervical cancer progression. Molecular biology techniques based upon amplification are the most sensitive methods for the detection of the viral DNA, especially in patients without apparent lesions, colposcopics changes or pathologic PAP smears. The aim of this study was to determine HPV infection, genotypes and viral load, in a cohort of 250 women in Mérida State (2008-2009) comparing a novel SYBR Green® quantitative real-time (q-RT) polymerase chain reaction (PCR) technology targeting the late-L1 viral genes with a end-point- PCR assay. Additionally, in order to detect HPV integration, we amplified viral E6/E7 ORFs as the target region by end-point PCR. In this assay, consensus primers including HPV18 and HPV16 genotypes were tested. Methods: Samples were collected in transport medium device (Digene®) and DNAs were isolated using QIAmp® DNA isolation Kit (QIAGEN®). Pap smear was performer by Papanicolaou tech. Results: End-point PCR of L1 region was apply to 125 samples, 20% (71/125) were positive for HPV and there was a moderated agreement (= 0.46) with the SYBR Green® q-RT PCR assay. Amplification of early E6/E7 region identified 19.2% (24/125) more positive cases, 50% (12/24) were HPV18, 37.5% (9/24) HPV16 and in 12.5% (3/24) amplification of more than one genotype occurred. Conclusion: In conclusion, target region for amplification of HPV DNA is a determinant factor in the molecular diagnosis of cervical infection. Moreover, quantification of the viral load by qRT- PCR SYBR Green-L1 assay allows to determines HPV infection, viral load, genotyping especially in women with high risks lesions by PAP smear.
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