14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 84.027 Session: Virology and Viral Infections (Non-HIV) Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Rotavirus diarrhea in Kashmir: Detection of genotype G12P[6] strains I. Ahmad SK University of Agricultural Sciences & Technology of Kashmir, Kashmir, Jammu & Kashmir, India Background: Rotavirus genotype G12P[6] was detected among children with acute gastroenteritis in Kashmir Valley-India. The study was conducted in two major hospitals of the Valley from Jan 2006 to Jan 2007. Six (6) cases of G12 infection were identified having P[6] specifity. Three (3) cases of G12 P[6] were detected by PCR and other three (3) by gene sequencing. Sequence analysis of the VP7 gene from three neonatal strains indicated a high level of aminoacid homology (98-99%) to other G12 strains reported world wide, suggesting introduction of novel rotavirus strain serotype into the community.The G12 strains were detected sporadically and were not associated with any outbreak or nosocomial infection.Genetic characterization of VP7 gene of these strains revealed one lineage and a very little aminoacid divergence
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 84.028 Session: Virology and Viral Infections (Non-HIV) Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Surveillance of rotavirus strains reveals evidence of emerging G12 and unusual human-animal reassortant strains in Manipur, North-eastern India A. Mukherjee, M. Chawla-Sarkar National Institute of Cholera and Enteric Diseases, Kolkata, India Background: The rotaviruses are the major cause of severe gastroenteritis and account for an estimated 100,000 deaths and 400,000 hospitalizations in children under 5 years of age in India. To date, 23 G and 31 P genotypes have been reported in Group A Rotaviruses on the basis of differences of the outer capsid VP7 and VP4 encoding genes. North-Eastern states of India are geographically distinct with temperate weather compared to rest of the India. Few outbreaks were reported during the year 1979 to 1988 in Manipur with evidence of zoonotic transmission but systematic surveillance study was not done till date. Methods: The samples were collected from children (98% identity with Bangladeshi, Thailand and USA strains. Few unusual G-P combinations like G4P[4], G4P[6], G10P[6] and G9P[19], along with some G-P nontypables were also found. G4 and G10 strains clustered with the porcine and bovine strains, indicating zoonotic transmission. Conclusion: High frequency of rotavirus infection ( 50%) and predominance of the common strains G1P[8] and G2P[4] among children with acute diarrhea emphasizes the need for implementation of currently available vaccines to reduce the burden of rotavirus induced diarrhea in high risk regions of developing countries.
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