14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 84.031 Session: Virology and Viral Infections (Non-HIV) Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Regulation of poly(A) binding protein during herpes simplex virus infection O. G. Larralde-Diaz 1 , R. W. P. Smith 2 , P. Malik 3 , S. V. Graham 4 , N. K. Gray 2 1 NHS, Scottish National Blood Transfusion Service, Edinburgh, United Kingdom, 2 Queens Medical Research Institute, Edinburgh, United Kingdom, 3 University of Edinburgh, Edinburgh, United Kingdom, 4 University of Glasgow, Glasgow, United Kingdom Background: The herpes simplex virus (HSV) ICP27 protein is an essential IE protein, conserved through the Herpesviridae family, that constitutes a target for the development of antiviral drugs and vaccines. ICP27 is a key regulator of viral and cellular gene expression, with most of its activities in the nucleus. Methods: The regions of ICP27-PABP1 interaction were mapped by GST pull down and immunoprecipitation, using a panel of recombinant HSV. PABP1 phosphorylation was studied by 2D-electrophoresis, while shuttling activity was shown by confocal microscopy. Tether function assay in Xenopus eggs was used to study ICP27 effects in translation, independtly of its nuclear activities. Results: ICP27-PABP1 interaction is partially affected by RNAse I treatment in vivo, which could be explained by the ability of both proteins to form multimers with RNA. Both purified proteins are able to interact directly in vitro in the presence of RNAse I. The C-terminal of ICP27 is required for this interaction, while PABP1 interacted through RRM1-2 and C terminal. We described here a novel role for ICP27 in the cytoplasm, where it stimulates translation. We showed that PABP1 activity is regulated early in HSV infection by phosphorylation, mediated by HSV UL13 kinase. These events may cause redistribution of ICP27 from the cytoplasm to the nucleus late in infection. Conclusion: Our results showed that PABP1 activity is regulated during HSV-1 infection by phosphorylation and relocalisation, events that seems to be linked to translation regulation. Experiments to elucidate the mechanism of translational control exerted by ICP27 are currently under investigation.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 84.032 Session: Virology and Viral Infections (Non-HIV) Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Complications of varicella in healthy children in Izmir, Turkey Z. Kurugol 1 , O. Halicioglu 2 , I. Devrim 3 , G. Koturoglu 1 , F. Vardar 1 , C. Dizdarer 3 , M. Helvacı 2 1 Ege University, Izmir, Turkey, 2 Tepecik Children Hospital, Izmir, Turkey, 3 Behcet Uz Children Hospital, Izmir, Turkey Background: Varicella is usually a benign and self-limited childhood illness. Complications are thought to be quite rare in immunologically healthy children. However, studies report that varicella epidemiology is changing and that the rate of hospitalization in immunocompetent children are more frequent than previously thought. The purpose of the study was to describe complications of varicella requiring hospitalization in a defined population (Izmir, Turkey) and to compare the complication of varicella with our published study in 1997-2001. Methods: Between 2005 and 2009, hospital records of patients admitted with complications of varicella to three children’s hospitals in Izmir (Ege University Children's Hospital, Behçet Uz, and Tepecik Children’s Hospital) were reviewed. Incidence and clinical spectrum of complications were analyzed. Results: From 2005 to 2009, 262 cases (median age, 3.8 years) were hospitalized for varicella complications, whereas 178 cases (median age, 3 years) were hospitalized between 1997 and 2001. This resulted in a crude incidence of 8.7/100 000 population at risk (6.3/100.000 previously). There was a seasonal distribution of complications with a peak in January. The most frequent complications were infectious complications, which were observed in 96 children (36.6%). Pneumonia was observed in 48 children (18.5%). Superinfections of the skin were present in 39 patients. A total of 93 (35.5%) neurologic complications were observed. Cerebellar ataxia was most frequent neurologic complication. Infectious complications occurred more frequently in younger children (median age: 2.5 years), whereas neurologic complications occurred at an older age (median age: 6.3 years). Hematologic complications were seen in 9 children (3.4%). Three cases (0.1% of hospitalized patient) were dead from complications of varicella (2 from encephalitis and 1 from pneumonia) between2005 and 2009, whereas no fatality has been reported during previously study period (1997-2001). Conclusion: The rate of hospitalization of children with complications of varicella is higher than described in our previously study.
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