14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 79.011
Session: Obstetrical-Gynecological, Surgical and Sexually Transmitted Infections
Date: Friday, March 12, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Detection of human Papilloma virus DNA in cervical samples in Maracaibo-Venezuela
D. Callejas, M. E. castellanos, R. Mindiola, Y. Carrero, F. Monsalve, L. Porto, R. Atencio
Universidad del Zulia, Maracaibo, Zulia, Venezuela
Background: Cervical cancer is the second leading cause of cancer death among women
worldwide, mainly in countries in process of development. There are multiple risk factors involved
in the genesis of pre-invasive lesions and cancer, such as sexual behavior, and some sexually
transmitted infections especially Human Papilloma Virus. Objective: To perform molecular
diagnosis of HPV
Methods: In a period from 2007 to 2009 have been treated 300 patients in Research Seminar in
different health centers, each underwent a medical history, sampling for cytology and a citobrush
(DNA collection device, Digene ®), for diagnosis of HPV by PCR using primers for L1 region
(MY09/11, GP5 + / 6 +)
Results: Of the patients attended 40.5% were positive for HPV infection, of which one 22.55%
were diagnosed by MY09/11 primers vs 31.98% GP5 + / 6 + sharing between 14,14% of cases
Conclusion: The PCR evaluated in this study showed significant differences, the use of primers
GP5 + / 6 + possible to identify a larger number of cases, which is very important. The use of this
technique applied in conjunction with colposcopy and / or cytology, are fundamental tools for
definitive diagnosis and monitoring of cervical HPV infection.