14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 73.014 Session: Animal Models, Pathogenesis & Host Defenses Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Transcriptome profile of the human endothelial cell response to high- and low-density infections of Candida albicans P. P. Chong 1 , C. S. Y. Lim 1 , Y.-H. Tan 1 , H. F. Seow 2 , R. Rosli 1 1 University Putra Malaysia, Selangor, Selangor, Malaysia, 2 Victoria University , Melbourne, Victoria, Australia Background: Candida albicans morphology switching and quorum-sensing are important factors for pathogenicity and virulence in persons with a compromised or deficient immune system. This study investigates the in vitro response of human umbilical vein endothelial cells (HUVECs) to infections with low and high densities of C. albicans. We hypothesize that higher cell densities of C. albicans yeast-form cells (blastospores), are more detrimental to HUVECs than lower cell densities of hyphal forms. Methods: Three biological replicates of confluent HUVECs in 6-well plates were challenged with 106 C. albicans blastospores (low-density infection) and 5 x 107 blastospores (high-density infection) for 8 hours. The low-density infection generated true hyphae, but in the high-density infection, C. albicans remained as blastospores. RNA from these samples were subjected to DNA microarray transcript profiling. For MTT and XTT cell proliferation assays, conditioned media from the co-cultures for microarray experiments were incubated with HUVECs in 96-well plates for 24 hours. Results: The high-density blastospore-HUVEC co-cultures elicited significantly higher differential expression of genes involved in functional pathways of apoptosis, immune response, cell-cell signaling and cancer development, such as ZC3HAV1, HES1, CSF2, CXCL2 and PIM1, compared to the low-density true hyphae-HUVEC co-cultures. Cell proliferation assays also show that HUVECs incubated with conditioned media from the high-density infection caused a higher percentage of cell death compared to incubation with conditioned media from the low-density infection. These results suggest that high densities of unattenuated, innate C. albicans blastospore cells can cause significant cellular toxicity, even though the cells are in the yeast form, not filamentous. Microarray Data Analysis Conclusion: Transcript profiling of this in vitro endothelial cell model may provide new insights into how C. albicans cell densities affect the host during the colonization and invasion through the bloodstream to the deep organs. We also suggest that quorum-sensing molecules and other unknown secretions from high-density C. albicans infections are strong inducers of cellular injury leading to cell death in systemic candidiasis.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 73.015 Session: Animal Models, Pathogenesis & Host Defenses Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Levels of pro-inflammatory cytokines and soluble cell adhesion molecules serves as early markers for recognition of diseases severity in patients with dengue fever and dengue hemorrhagic fever M. Odreman 1 , S. Vielma 1 , C. Torres 1 , L. Tellez 2 , J. Mendoza 2 , S. Perez 1 , N. Mosqueda 1 , M. Muñoz 1 , J. Muñoz 1 , J. Goyo 1 1 Universidad de Los Andes, Facultad de Medicina, Merida, Merida, Venezuela, 2 Universidad de Los Andes, Merida, Merida, Venezuela Background: Several mechanisms have been proposed to explain the pathogenesis of dengue virus infection. One of the most important finding is the production of pro-inflammatory cytokines as responsible of activation of vascular wall, plasma leakage and subsequently disease severity. Our aim was to determine levels of pro-inflammatory cytokines during the course and severity of dengue infection. Methods: Seventeen febrile patients with dengue infection (RT-PCR and IgM-anti-dengue) were included and classified by diseases severity (OMS). Dengue fever (DF) were observed in 7/17 cases (2 Adults, 22-56 years old, 39 ± 24.04; and 5 children 3-11 years old, 7.60 ± 3.78); and ten cases had Dengue Hemorrhagic Fever (DHF, 2 Adults; 22-47 years-old, 34 ± 17.67 and 8 children, 5 months-12 year-olds, 4.52 ± 4.27). Two afebrile patients were included as controls. At least two serum samples were collected during either acute phase (0.05) and sTNF- (p=0.026) were significant higher in DHF cases during the critical phase (4d) of the diseases. High soluble levels of ICAM-1 were observed in FD and DHF cases, with a significant differences (p=0.025) during critical phase of the disease. Moreover, significant soluble levels of VCAM-1 were observed in both cases of DF (p=0.013) and DHF (p=0.011) during the early stages of the clinical course (
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