14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 74.017
Session: Antibiotic Resistance: Gram-Positive
Date: Friday, March 12, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Resistance to antibiotics of acinetbacter strains isolated from hospital associated infections
L. M. Junie 1 , E. Papadomanolaki 2 , G. Aleuraki 2 , P. Karagianni 2 , A. Tsafaraki 2 , A. Tsouri 2 , D.
Labousaki 2 , E. Volanis 3 , S. KASTANAKIS 2
1 University of Medicine and Pharmacy , 43200, Romania, 2 St. George General Hospital Chania,
Chania, Greece, 3 Infection Control Committee "St. George" General Hospital, Chania, Greece
Background: Acinetobacter infection is an emerging problem, as multiple resistant strains to
antibiotics colonize Intensive Care Units. The objective was to study the resistance patterns of
Acinetobacter strains isolated in ICU and other wards.
Methods: Acinetobacter strains were isolated from Intensive Care Unit (ICU) and from others
clinics (OC) in Chania, Greece, representing 3.06% of the total positive blood cultures. Three
blood samples were taken from each patient in bottles of Bact-Alert system (Bio-Merieux). 68
strains were isolated from patients with urinary tract infections. Identification was carried out with
API 20E and Vitek2 method. Antibiotic sensitivity testing of strains was performed by disk
diffusion method (Kirby Bauer) as recommended by CLSI and Vitek 2 system.
Results: Acinetobacter strains isolated in ICU showed a high percent of resistance (84.6-100%)
to Pip, Caz, Imp, Taz, Cip (76.9%), Akn (61.5%), lower percent of resistance (53.8) to Mer and
Sam and only 30.7% were resistant to Gen. 87.5% of Acinetobacter strains isolated in OC were
resistant to Pip, Caz and Cip, 62.5% to Akn, Gen, Taz, Imp, 50% to Mer and only 25% to Sam.
Acinetobacter strains isolated in OC showed lower percent of resistance to Sam, Imp, Taz, Caz,
Pip and higher to Cip and Gen. No significant difference exists in resistance among strains
isolated in ICU and OC to Mer, Akn. 25%-50% of Acinetobacter strains isolated from urine were
resistant to Am, Pip, Azt, Caz, Gen, Cip. They were resistant also to Nalidixic acid and
Nitrofurantoin. They remain still susceptible to Imp, Mer, Ureidopenicillin+ lactamase inhibitor,
Ticarcillin/Clavulanic acid (CA), to Cefpodoxime and some third (Ceftriaxone) and fourth
generation cephalosporin’s (Cefepime).
Conclusion: Aminoglycosides, Quinolones and most of Lactamins are no more indicated in the
therapy of Acinetobacter produced infections.The presence of multiple resistant strains in ICU is
attributed to invasive procedures and the use of broad-spectrum antimicrobials. It is rather difficult
to distinguish morbidity and mortality attributable to Acinetobacter from that attributable to the
common and severe co-morbidity in these patients (ICU). Therefore good clinical evaluation is
essential to avoid unnecessary treatment. Infection control measures are crucial for limiting
spread and alternative therapies with ampicillin/sulbactam are an option that needs further study.