14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 79.002 Session: Obstetrical-Gynecological, Surgical and Sexually Transmitted Infections Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Syphilis and HIV co-infection in Thai Medical School Hospital N. Hiransuthikul Faculty of Medicine, Chulalongkorn University, 10330, Thailand Background: HIV and syphilis affect similar patient groups and co-infection is common. In HIV infected patients, clinical manifestation of syphilis may be atypical and different from HIV uninfected cases. This study aimed to compare the characteristics of syphilis between HIVseropositive and HIV- seronegative patients in Thai Medical School Hospital Methods: Retrospectively chart review of new cases of syphilis who attended the STD clinic, Chulalongkorn Hospital, Bangkok,Thailand from 1st June 2001 to 31st May 2006 was conducted. Results: There were totally 385 new cases of syphilis during the studied period and HIV screening was done in 325 patients.The prevalence of HIV infection among syphilis patients was 33.2%. We found that younger age, male gender, and men who have sex with men (MSM) were significantly associated with HIV infection (p
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 79.003 Session: Obstetrical-Gynecological, Surgical and Sexually Transmitted Infections Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Genotyping of Chlamydia trachomatis and human papillomavirus in clinical specimens from North-Eastern Croatia Z. Bosnjak 1 , M. Peric 1 , D. Pavlinic 2 , S. Dzijan 3 , I. Roksandic-Krizan 1 , D. Vukovic 1 , N. Ruzman 1 , J. Burazin 1 , G. Lauc 3 , A. Antolovic-Pozgain 1 1 INSTITUTE of Public Health for Osijek-Baranya County, Osijek, Croatia, 2 School of Medicine, Osijek, Croatia, 3 School of Medicine, DNA Laboratory, Osijek, Croatia Background: Human papillomavirus (HPV) and Chlamydia trachomatis (Ct) are major causes of sexually transmitted diseases. Clinical consequences of high-risk HPV (HR HPV) infection, especially with HPV types HPV 16 and HPV 18, include abnormal Pap test results, low and highgrade cervical intraepithelial neoplasia and cervical cancer, for which Ct may sometimes be a cofactor.The aim of the study was to determine the prevalence of Ct and age-related profiles of HR HPV infections among cases of other sexually transmitted infections in our county. Methods: Due to the chronic and “silent” infection of Ct, fast molecular diagnostics and adequate therapy of the infected individuals are the crucial steps in the Ct spread control. During three-year period we tested 2327 genitourinary samples for Ct and 945 gynaecological samples for HR HPV with normal and abnormal cervical cytological diagnosis. Subsequently all Ct positive samples were analysed by sequencing of the amplified omp1 fragments using Applied Biosystem 3130 Genetic Analyser. Genotyping and sequence mutation analysis were performed using ABI SEQSCAPE software and compared with the reference sequences of all known Ct serotypes. HR HPV positive samples with abnormal cervical cytological diagnosis were genotyped by Linear Array HPV Genotyping Test (Roche Diagnostics). Results: The determined serotype of Ct and HPV genotypes distribution were compared with the Ct and HPV distribution pattern in other regions of the world. The association between certain HPV genotype and cervical intraepithelial neoplasia was determined as well as mixed infections with four or more HPVs. The most prevalent Ct genotype in Osijek-Baranya County was serotype E (in concordance with Sweden and Taiwan data), followed by F, K, G, D, B and J (differs from Sweden and Taiwan data). The sequence of omp1 gene showed limited variation. HPV 16 and HPV 18 were the most common HR HPV genotypes in the cervical specimens with abnormal cervical cytological diagnosis. Obtained results also determined mixed infection of four HR HPV with one or more low risk HPV. Conclusion: The obtained results for Ct and HPV and further analyses in this ongoing project could be useful tool for clinical and epidemiological characterization of circulating pathogens in our community.
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