14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 76.018
Session: Emerging Infectious Diseases
Date: Friday, March 12, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Epidemiological, clinical and zoonotic evidences for the existence of Lyme disease in Central of
Mexico
G. GORDILLO 1 , F. Solorzano Santos 2 , J. Torres 3 , E. Velazquez 2 , G. Ramon 2 , R. Garcia 2 , M.
Vargas 4
1 INSTITUTO MEXICANO DEL SEGURO SOCIAL, MEXICO CITY, Mexico, 2 Hospital de pediatria
, Centro Medico Nacional SXXI, IMSS, Mexico, DISTRITO FEDERAL, Mexico, 3 HOSPITAL DE
PEDIATRIA CENTRO MEDICO NACIONAL SXXI , IMSS, MEXICO, DISTRITO FEDERAL,
Mexico, 4 Facultad de Medicina Veterinaria y Zootecnia, Mexico, Michoacan, Mexico
Background: Lyme borreliosis, caused by Borrelia burgdorferi, is the most commonly reported
vector-borne disease in the United States. Infection is transmitted to humans by Ixodes tick-bite,
and causes a disease affecting skin, nervous system, heart, and joints. Recent studies in Mexico
documented cutaneous clinical cases infected with B. burgdorferi and ticks from the Northeast of
the country infected with B. burgdorferi sensu stricto. Aim: To document Borrelia infection in
patients with suggestive Lyme disease in Central of Mexico, and to search for the presence of B.
burgdorferi-infected competent vectors and reservoirs.
Methods: We studied 800 patients suggestive of Lyme disease in one Pediatric and one General
hospital. Sera, biopsies, and cerebrospinal fluids were tested for infection using immunological,
microbiological, and molecular test. Ticks and mice were collected in forest areas, which patients
visited, and infection studied by culture and molecular tests.
MEXICAN PATIENT WITH EM
Results: From 800/136 cases (17%) fulfilled the CDC criteria confirming B. burgdorferi infection,
88 adults (64.5%) and 48 children (35.5%). Eighty-one presented neurological manifestation
(59.6%), 45 cutaneous lesions (33%), and 10 arthritis (7.4%), and 68 reported previous tick
exposure (50%). Thirty-six Peromyscus sp. and 17 Neotomodon alstoni mice were collected in
forest areas; five P. melanotis were found infested with Ixodes spinipalpis ticks. In 16 mice,
samples from bladder and articulation were tested; five were culture+ and seven PCR+ for fla and
ospA genes. Amplified fla and ospA genes from clinical, rodent and tick samples were sequenced
and confirmed as Borrelia burgdorferi sensu stricto genes.
Ixodes spinipalpis tick infesting Peromyscus mouse
Conclusion: These studies confirm for the first time the existence of this emerging zoonosis in
Latin America, and leaves open the intriguing possibility that this represent an example of the
epidemiological transition due to world climate changes.