14th ICID - Poster Abstracts - International Society for Infectious ...

More documents

Recommendations

Info

When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 75.012 Session: Diagnostics Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation An improve dot enzyme immunoassay for serodiagnosis of melioidosis S. A. K. Zainoodin 1 , I. Asma 2 1 Advance Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia, 2 Deputy Vice Chancellor, Universiti Sains MAaaysia, Georgetown, Penang, Malaysia Background: Meliodosis in an infectious disease affecting multi-organ system of man and animals caused by a bacterium, Burkholderia pseudomallei. The disease is potentially fatal and early detection is crucial for institution of life saving antimicrobial therapy. Culture isolation remains as a mainstay for definitive laboratory diagnosis of melioidosis. But it is less sensitive, laborious and time-consuming task. In this report, an improved dot EIA test (patent pending) with user friendly assay protocol for serodiagnosis of melioidosis is described. Methods: Two optimised concentrations of antigens were dotted onto a nitro-cellulose membrane (Microfiltration System, Ca, USA, 0.45uM) divided into 9mm x 4mm. The membranes were blocked for 30 minutes in tris-buffered saline (TBS) wiht 5% non-fat skim milk. The membranes were rinsed and cut according to the divided size into test strips and placed in 48 well flat tissue culture plates (Costar 3548, UK.) The test strips were then incubated with patient serum at 1:100 in TBS for 1 hour at room temperature. After incubation, the test strips were washed with TBS for three times and each washing was done for 5 minutes. Subsequently, the diluted alkaline phosphatase conjugated goat anti-human immunoglobulin IgM, IgG and IgA isotypes were added into the respective wells and incubated for 1 hour at room temperature. After incubation the strips were washed three times in TBS as described above. Chromogenic substrate was used in the colour development reaction. Aftwer after 15 minutes the strips washing with distilled water to stop coloue development. The positive and negative results were determined by visual comparison of the dot intensity for each test with the positive cut off control. Results: The sensitivity and the specificity of the assay was 95% and 92%, respectively for the detection of IgG, IgM and IgA antibody isotypes. However, detection of IgG alone showed sensitivity value of 89.2%. The sensitivity value for the detection of IgM alone and IgA alone were 47.7% and 60% respectively. Dot EIA test results Conclusion: The results showed that combined detection of all three antibody isotypes are required to increase the sensitivity of the assay. In addition, the result from the propective study showed that the test was very useful for early and accurate diagnosis melioidosis in clinical cases
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 75.013 Session: Diagnostics Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Diagnostic value of Elisa serological test using synthetic peptides of Mycobacteriun tuberculosis antigens in childhood tuberculosis D. LOPEZ 1 , F. GIAMPRIETO 2 , J. DE WAARD 2 , M. A. Patarroyo 3 , C. Fernandez de Larrea 4 , Z. Araujo 5 1 JM DE LOS RIOS HOSPITAL, CARACAS, DISTRITO CAPITAL, Venezuela, 2 INSTITUTO DE BIOMEDICINA, CARACAS, DISTRITO CAPITAL, Venezuela, 3 Fundación Instituto de Inmunología de Colombia, Bogotá, Colombia, 4 Servei d’Hematologia. Hospital Clínic de Barcelona, Barcelona, Spain, 5 Instituto de Biomedicina, Universidad Central de Venezuela, 4043, D.C., Venezuela Background: One study was conducted to evaluate the efficacy of ELISA serological test for the detection of IgG antibodies against ESAT-6 synthetic peptides and the recombinant ESAT-6 antigen (rESAT-6). Methods: The study was carried out on children with pulmonary tuberculosis and healthy controls (13 cases and 63 controls). Informed consents of their parents or guardians were taken. They were subjected to clinical examination, relevant laboratory investigations, tuberculin test (epidemiological link with confirmed case), chest radiograph and therapeutic positive test. Relevant body fluids (sputum and gastric fluid) were subjected to biochemical test adenosine deaminase (ADA) and bacteriological tests (Lowëstein-Jensen (LJ) and Ziehl Neelson (ZN)) and PCR. Sera samples were analyzed for antibodies against 5 ESAT-6 synthetic peptides (12033, 12034, 12035, 12036 and 12037) and rESAT-6. The Utility of these methods was evaluated using receiver operating characteristic curve (ROC) analysis. Results: ELISA test using ESAT-6 peptide (anti-12036) showed a significantly higher sensitivity (100%) than ADA, LJ medium culture method and ZN staining and PCR tests (30% ADA, 0% ZN staining, 0% LJ culture and 12.5% PCR) in all patients (p < 0.001). ADA biochemical test was highly specific (100%), followed by anti-12033 test (95.2%). Anti-rESAT-6 showed been lowly sensible and specific. Conclusion: In view of the convenience, low cost and high sensitivity and specificity, the ELISA tests using a combination of ESAT-6 peptides could have a promising future in the diagnosis of childhood tuberculosis.
Page 1 and 2: When citing these abstracts please
Page 47: When citing these abstracts please
Page 99 and 100:
When citing these abstracts please
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Page 267 and 268:
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
show all

14th ICID - Poster Abstracts - International Society for Infectious ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?