14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 75.010 Session: Diagnostics Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Specificities of the APTIMA Combo 2 and ProbeTec for Chlamydia trachomatis and Neisseria gonorrhoeae in oropharyngeal and rectal specimens from MSM J. Schachter 1 , J. Moncada 1 , A. Roger 1 , L. Rauch 2 , S. Liska 2 , C. Shayevich 2 , J. D. Klausner 2 1 University of California, San Francisco, San Francisco, CA, USA, 2 San Francisco Department of Public Health, San Francisco, CA, USA Background: Nucleic acid amplification tests (NAATs) are not FDA-cleared for diagnostic testing of chlamydial (CT) or gonococcal (GC) infection in extragenital sites. In men who have sex with men (MSM), CT and GC infections of the oropharynx or rectum may be common. There have been some concerns about false-positive NAAT results from these sites. We evaluated the APTIMA Combo2 (AC2, Gen-Probe Inc.) and ProbeTec (SDA, Becton Dickinson, Co.) performance on these specimens. Here we present the specificity results. Methods: Oropharyngeal and rectal swabs were obtained from MSM in an STD clinic and SDA and AC2 were performed on all specimens. NAAT positive specimens were retested by AC2, SDA and by APTIMA CT Assay (ACT), or APTIMA GC Assay (AGC) which target rRNA sequences different from AC2. Results: We tested 1110 MSM. AC2 had more positive results than SDA for both organisms at both sites. The number of positive results, and the percentage confirmed are presented in the table. Only 11 oropharyngeal CT infections were detected, but the number of positive GC specimens and rectal CT specimens was larger allowing more confidence in the reliability of the confirmation data. Using a combination of all 3 tests confirmed >90% of positive samples, resulting in high specificities (>99.6%) for both AC2 and SDA. SDA AC2 CT GC CT GC (N) Phx (6) Rec (43) Phx (69) Rec (70) Phx (11) Rec (67) Phx (81) Confirmed by (%): AC2 or 100* 93 83 99 55 60 70 81 SDA ACT or 100 91 98** 99† 82 93 91 94 AGC Repeat 100 98 91 97 82 93 91 94 test All 3 100 100 94 99 91 94 98 98 Rec (85) % confirmed, **only 57 tested by AGC, † 1 not tested by AGC. Conclusion: In our MSM population, positive results obtained with AC2 and SDA are reliable, with PPVs >90%. The CT and GC NAAT positives were confirmed to a high degree. As expected, repeat testing, or using the ACT or AGC test, confirm more of the AC2 positive results than did the less sensitive SDA.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 75.011 Session: Diagnostics Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Reverse transcritpase multiplex PCR for detection of viral agents in central nervous system infections V. Gopalakrishnan 1 , H. Amar Singh 2 , T. Sadanandan 1 , G. Perkash Singh 1 1 UniKL RCMP, Ipoh, Malaysia, 2 Consultant Paediatrician and Head of Paediatric Department, Raja Permaisuri Bainun Hospital, iPOH, Malaysia Background: Viral infections of the central nervous system (CNS) may result in clinical syndromes like aseptic meningitis, encephalitis, and myelitis. These are often difficult to diagnose using conventional laboratory methods, such as viral culture and serology, because they are time consuming and unsatisfactory. Therefore rapid techniques should be employed to detect the etiologic agent. The study was aimed to standardize reverse transcriptase (RT) multiplex PCR aimed to detect viral etiology in CNS infections. Methods: An RT multiplex PCR designed to detect, viral etiologies, enterovirus, herpes simplex and varicella zoster viruses in CNS infections has been standardized. Three sets of primers were been employed for their detection. Amplification of target sequences was qualitatively analyzed by looking for the presence or absence of amplicons on agarose gel. The RT multiplex PCR was standardized. Sensitivity of the PCR has been ascertained. Results: Analysis of cerebrospinal fluid samples from pediatric patients is underway. Conclusion: The RT multiplex PCR standardized can be employed to detect CNS infections caused by herpes, varicella and entero viruses.
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