14th ICID - Poster Abstracts - International Society for Infectious ...

More documents

Recommendations

Info

When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 82.007 Session: Trypanosomiasis, Leishmaniasis & Schistosomiasis Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Patients with suspected visceral leishmaniasis in Istanbul H. Cakan 1 , S. Saribas 2 , V. Oz 1 , E. Polat 3 , M. Aslan 2 , B. Kocazeybek 3 1 Istanbul University, Forensic Medicine Institue, Istanbul, Turkey, 2 Istanbul University Cerrahpasa Faculty of Medicine, Istanbul, Turkey, 3 Istanbul University Cerrahpasa Faculty of Medicine, Turkey, Turkey Background: Visceral Leishmaniasis (VL) is a parasitic disease caused by Leishmania infantum. It is transmitted through bites of infected sand flies (female Phlebotomus). We aimed to investigate bone marrow and blood samples obtained from the patients with suspected VL. Methods: Fifty-nine patients with suspected VL from Istanbul were included in this work. Bone marrow and blood samples of these patients were tested for possible VL infection using several methods including serological tests, microscopy, PCR. Results: Nineteen (32.2%) patients had positive results for VL after one or more of the tests performed, while only 7 patients (11.8%) had positive results with all the tests including Giemsa stain. Four (6.8%) patients had negative results based on all the serological tests performed except for positive results with Giemsa stain, culture and PCR. The other 4 (6.8%) patients had positive results with Formol-gel, ELISA IgG ( >1.1 ISR) and IFAT IgG, (>1/256) but negative results were obtained with direct microscopic examination, culture and PCR. Using PCR Leishmania infantum DNA was detected in 11(18.6%) of the (Leishmania) cultures originated from the bone marrow samples. Plasmodium vivax was found in 2 (3.4%) patients and leptospira was detected in 1 (1.7%) patient. One (1.7%) patient was diagnosed with Pneumonia (Streptococcus pneumoniae). Forty (67.8%) patients had negative results after direct microscopic examination, culture, serological tests and PCR. The kappa coefficients =0.80 = 1.00, =0.51, =0.55 and =0.45 were evaluated for PCR and direct microscopic examination, PCR and culture, PCR and ELISA, PCR and IFAT and PCR and Formol-Gel, as perfect agreement, perfect agreement, moderate agreement and moderate agreement fair moderate, respectively. The probability values (p) for comparisons of all the above tests with PCR showed a significant correlation (p
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 82.008 Session: Trypanosomiasis, Leishmaniasis & Schistosomiasis Date: Friday, March 12, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Immunological profile of CD18-deficient mice during Schistosoma mansoni infection M. S. Espíndola, F. G. Frantz, L. H. Faccioli Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Ribeirão Preto, SP, Brazil Background: Schistosomiasis is recognized as the most important human helminth infection in terms of morbidity and mortality harboring around 200 million people world-wide, beeing cosidered a risk for travelers. A study focusing the role of integrins, which are involved on cellular migration, antigen presentation and T cell activation, is necessary on the knowledge of immunopathology during schistosomiasis. The aim of this work is to evaluate the role of CD18 molecule, a 2 integrin, in modulate the immune response and pathology during the development of experimental schistosomiasis. Methods: C57BL/6 (WT) mice and CD18low mice were percutaneously infected with 50 cercariae and the parasitological evaluation was done 48 days after infection. The adult worms were recovered from the hepatic portal system and the liver by perfusion with citrate saline. Ten and 48 days after infection, the cellular recruitment to the bronchoalveolar lavage fluid (BALF), as well the number of inflammatory cells present on the peripheral blood and the cytokines production in the lung homogenates were evaluated. To determine the proliferation of T CD3+CD4+ cells and the cytokines production in vitro, splenocytes were stimulated with concanavalin-A. Results: CD18low mice showed an increased susceptibility to infection with S. mansoni since the worm burden was 135% higher than in the WT group. Nevertheless, the cellular recruitment to the BALF was similar between WT and CD18low mice, while CD18low mice showed a markedly enhancement on the accumulation of mononuclear cells in the peripheral blood, suggesting that less effector cells could migrate through blood to the inflammatory focus. Moreover, T cells from CD18low mice presented reduced potential to proliferate in the presence of Con-A than cells from infected WT mice. Ten days after infection the measurement of TNF-, IL-12, IL-5, IL-10 and IL-4 in the lung homogenates was always lower in CD18low mice. Although, 48 days after infection, only IL-5 and IL-12 in CD18low mice showed slightly inferior levels. After in vitro stimulation of splenocytes with Con-A, just IL-5 production from CD18low mice was lower than WT. CD18low mice are more susceptible to infection with S. mansoni than WT mice. Worm burden was obtained by perfusion of the hepatic portal system with citrate saline. ** p
Page 1 and 2:
When citing these abstracts please
Page 3 and 4:
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182: When citing these abstracts please
Page 231: When citing these abstracts please
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
show all

14th ICID - Poster Abstracts - International Society for Infectious ...

Create successful ePaper yourself

Delete template?

Save as template?