14th ICID - Poster Abstracts - International Society for Infectious ...

More documents

Recommendations

Info

When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 83.021 Session: Vaccines and Vaccine Development Date: Thursday, March 11, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Effectiveness of influenza vaccine in elderly people with chronic pulmonary and cardiovascular diseases in Argentina F. Nacinovich 1 , P. BONVEHI 2 , R. Ruttimann 1 , D. Stamboulian 3 1 FUNCEI, Buenos Aires, Argentina, 2 CEMIC, BUENOS AIRES, Argentina, 3 FUNCEI; Clinical Director, Ciudad Autonoma de Buenos Aires, Argentina Background: Influenza immunization proved to be effective in decreasing pneumonia hospitalizations in the elderly and in patients with high-risk conditions. The purpose of this study was to assess the effectiveness of influenza vaccine in persons 65 years with chronic pulmonary and cardiovascular diseases during four immunization campaign seasons in Argentina. Methods: Influenza vaccines containing the strains specifically recommended by the WHO were administered free of charge in immunization campaigns from 1994 to 1999. During campaigns, demographic data and disease status of each subject who was immunized was obtained through interview before vaccine was administered. Subjects with chronic pulmonary (CPD) and cardiovascular disease (CVD) were retrospectively evaluated for hospitalization due to pneumonia during the previous influenza season (June-October;1995-1998) and vaccination status. Results: A total of 176.778 people with CVD and 82.385 with CPD were evaluated. Annual influenza vaccine effectiveness for reduction of pneumonia hospitalization was: 1995 % (95%CI) 1996 % (95%CI) 1997 % (95%CI) 1998 % (95%CI) CVD 43 (33-52) 36 (28-46) 29 (18-38) 37 (26-46) CPD 41 (32-48) 35 (27-41) 27 (19-35) 27 (18-35) Conclusion: Influenza vaccination is associated with a significant reduction in hospitalization rates due to community acquired pneumonia in the elderly population in Argentina, particularly in those with high-risk conditions. Widespread use of influenza vaccine should be promoted.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 83.022 Session: Vaccines and Vaccine Development Date: Thursday, March 11, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Development and immunogenicity of a novel polyetherimine (PETIM) dendrimer based nanoformulated DNA rabies vaccine M. Shampur 1 , U. Padinjarenmattathil 2 , A. Desai 3 , J. Narayanaswamy 4 1 National Institute of Mental health and Neurosciences (NIMHANS), 560029, India, 2 National Institute of Mental Health & Neuroscience, Bangalore, India, 3 National Institute Of Mental health & Neurosciences, Bangalore, India, 4 Indian Institute of Science, Bangalore, India Background: Rabies is a fatal but preventable. In the past decade attempts have been made to develop an effective DNA vaccine against rabies. However, the effectiveness of plasmid DNA vaccines is limited due to ineffective intracellular gene delivery. Recently, a group of compounds called dendrimers have been shown to mediate effective gene delivery. In this study we have developed a novel, poletherimine (PETIM) dendrimer-based nano-formulated plasmid DNA rabies vaccine and evaluated its immunogenicity. Methods: The full length glycoprotein gene of rabies virus was cloned into the expression vector pIRES and its expression verified. Fourth generation amine-terminated polyetherimine (PETIM) dendrimer was synthesized and characterized. PETIM-pDNA complexes were prepared at varying ratios and their size, shape, transfection efficiency and cytotoxicity were studied. In vitro transfection experiments were conducted to identify the formulation possessing maximal transfection ability. The ability of the dendrimer to protect the encapsulated DNA was studied by deoxyribonuclease protection assay. The in vivo efficacy of the dendrimer-DNA complexes was evaluated by studying the virus neutralizing antibody responses in Swiss albino mice immunized with these formulations, in comparison with those produced by unmodified plasmid DNA. Results: The PETIM dendrimer was found to be non-toxic to cultured mammalian cells upto 1mg/mL. It was also observed to protect the complexed DNA from nuclease degradation.The PETIM-DNA complexes were also observed to produce satisfactory expression of the encoded gene in transfected cells. The animals immunized intramuscularly with the dendrimeric formulations of plasmid DNA produced adequate titres of protective, rabies-virus neutralizing antibodies by 2 weeks post-vaccination, after a single dose. The mean titre of neutralizing antibody was higher (1:512) in mice immunized with the dendrimeric formulations than in the group immunized with the unmodified plasmid DNA (1:16) (p
Page 1 and 2:
When citing these abstracts please
Page 3 and 4:
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210: When citing these abstracts please
Page 259: When citing these abstracts please
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
show all

14th ICID - Poster Abstracts - International Society for Infectious ...

Create successful ePaper yourself

Delete template?

Save as template?