14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 75.003
Session: Diagnostics
Date: Friday, March 12, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Sensivity and specifity of ELISA test for diagnosis of brucellosis
Z. Vakili, M. Momen Heravi, A. sharif
kashan university of medical sciences, kashan, isfahan, Iran, Islamic Republic of
Background: Brucellosis is a common disease with different clinical features and diagnostic
methods .Regarding to increasing use of ELISA as a new method this study was conducted to
determine sensivity and specifity of ELISA method for diagnosis of brucellosis in Kashan –Iran in
2007.
Methods: This study was done on 457 of persons with clinically suspected brucellosisThe
agglutination tests wright, coombs wright and 2ME as a standard tests and IgG and IgM ELISA
were done. Specifity and sensivity of ELISA was compared with standard tests.
Results: Sensivity of IgG and IgM ELISA was 93.7% &12.5%. Specifity of IgG and IgM ELISA
was 70.6% & 100%.positive predictive value IgG and IgM ELISA was 19.3% &100%.negative
predictive value of IgG and IgM ELISA was 99.3% &94%.
Conclusion: Regarding to high sensivity and false positivity of IgG ELISA and Low sensivity of
IgM ELISA, strict rely to ELISA for diagnosis of brucellosis may cause confusion of physicans.So
it must be used along by agglutination tests and clinical findings for confirmation of diagnosis of
brucellosis.