GTMB 7 - Gene Therapy & Molecular Biology

Spencer and Davie: Dynamic histone acetylation and its involvement in transcriptionA recent study mapping the distribution of di-methylatedlysine 9 on H3 across the chicken β-globin domain duringerythropoiesis showed that regions enriched in methylatedlysine 9 were depleted of di-acetylated H3 (K9 and K14).However, H3 acetylation correlated with lysine 4methylation, suggesting that transcriptional activation isassociated with H3 methylated at K4, as well as withacetylated H3 and H4 isoforms (Litt et al, 2001).Likewise, in Tetrahymena, methylated Lys4 of H3 isfound only in transcriptionally active macronuclei (Strahlet al, 1999).AcknowledgmentsResearch supported by grants from the CanadianInstitutes of Health Research (CIHR) (MT-9186,RO-15183), CancerCare Manitoba, and the U.S. ArmyMedical and Materiel Command Breast Cancer ResearchProgram (#DAM17-00-1-0319), and the National CancerInstitute of Canada with funds from the Canadian CancerSociety. A CIHR Senior Scientist Award to J.R.D. and aU.S. Army Medical and Materiel Command Fellowship toV.A.S. are gratefully acknowledged.ReferencesAfshar G, and Murnane JP (1999) Characterization of a humangene with sequence homology to Saccharomyces cerevisiaeSIR2. Gene 234, 161-168.Agalioti T, Lomvardas S, Parekh B, Yie J, Maniatis T, andThanos D (2000) Ordered recruitment of chromatinmodifying and general transcription factors to the IFN-βpromoter. Cell 103, 667-678.Annunziato AT, and Hansen J C (2000) Role of histoneacetylation in the assembly and modulation of chromatinstructures. Gene Expr 9, 37-61.Bannister AJ, Miska EA, Gorlich D, and Kouzarides T (2000)Acetylation of importin-alpha nuclear import factors byCBP/p300. Curr Biol 10, 467-470.Barbaric S, Walker J, Schmid A, Svejstrup JQ, and Horz W(2001) Increasing the rate of chromatin remodeling and geneactivation--a novel role for the histone acetyltransferaseGcn5. 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