GTMB 7 - Gene Therapy & Molecular Biology

Gene Therapy and Molecular Biology Vol 7, page 65Figure 5: Delayed injection of MnSOD-PL provides detectable protection from irradiation-induced organizing alveolitis/fibrosis.C57BL/6J mice were injected with MnSOD-PL 24 hours before 20 Gy irradiation to the pulmonary cavity. Subgroups of the MnSOD-PL-injected mice were given a second injection of MnSOD-PL at day 80, 90 or 100. Subgroups of non-injected but 20 Gy irradiatedcontrol mice were injected with MnSOD-PL only at day 1, 80, 90 or 100 following irradiation. Once 80% of the non-injected control,irradiated mice had been sacrificed due to moribund condition resulting from organizing alveolitis/fibrosis, representative mice in eachother group were sacrificed. The lungs were expanded in OCT, excised, frozen in OCT, sectioned, and H&E-stained. Representativephotographs of the lungs at the time of sacrifice are shown for: non-irradiated mice (A); 20 Gy non-injected control, irradiated mice, (B);20 Gy irradiated mice injected with MnSOD-PL at 80 days, (C); MnSOD-PL-injected mice 24 hours before 20 Gy, (D); or mice injectedwith MnSOD-PL both 24 hours before irradiation and again at day 80 (E).65