GTMB 7 - Gene Therapy & Molecular Biology

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Almazán et al: Expressed sequence tags in Ixodes scapularis4D11 Proteasome subunit 60%4D12Proteasome/Signalosomesubunit36% Mus musculus XP_13711855%43%56%4E7 Proteasome subunit 84%85%Drosophila melanogasterMus musculusAnopheles gambiaeMus musculusAnopheles gambiaeMus musculusNP_524115NP_035315EAA11895AAC33900EAA10351NP_036096The sequences of I. scapularis ESTs identical to ribosomal proteins pooled for DNA vaccination as described in Almazán et al. (2003),were compared to all non-redundant sequences in GenBank DNA and protein databases (1,419,727 sequences total; Apr-09-2003) usingBLASTX 2.2.6 (Altschul et al, 1997). The percent of identical amino acids to arachnida or insect and mouse sequences are showntogether with their corresponding GenBank accession number. The GenBank accession numbers for I. scapualris sequences are shownon Table 1.1 50M. musculus (1) ------------------------MAGACGKPHMSPASLPGKRRLEPDQED. melanogaster (1) MTKKLALKRRGKDSEPTNEVVASSEASENEEEEEDLLQAVKDPGEDSTDDH. sapiens (1) ----------------------------SVRPEKRRSEPELEPEPEPEPPA. gambiae (1) ---------------------QENLLGSIENEGEDSSDSDGEYATDDDEDI. scapularis (1) ----------------------MGPKTLSKQPAKASSSTSKRTAGPTISKConsensus (1) P S E A D D D51 100M. musculus (27) LQIQEPPLLSD-PDSSLSDSEESVFSGLEDSGSDSSEEDTEGVA----GSD. melanogaster (51) EGIDQEYHSDSSEELQFESDEEGNYLGRKQSSSAEEDEESSDEEDN---EH. sapiens (23) LLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDSSEDDDEGDEEGEDGAA. gambiae (30) DVLSFESLNSDGEE---EDEEEDAGTTLEEVEREAEEDDDEEDAERKQREI. scapularis (29) QTEDSDDEGSSSAYSDLEDSEGADSSDSNDLSDTEASEDDYDDSQDEENTConsensus (51) I E SS DS LEDSEES FSGLEDS SDSSEEDDEDDAE101 150M. musculus (72) SGDEDNHRAEETSEELAQAAPLCSRTEE--------------AGALAQDED. melanogaster (98) EEESTDGEEVEDEEKDSKSKQTDDKPSGSGAASKKALTAELPKRDSSKPEH. sapiens (73) LDDEGHSGIKKTTEEQVQASTPCPRTEM--------------ASARIGDEA. gambiae (77) EQFESDDEPLPDDLKLGRIEDVLGTGEKKTRGLGVFPPVPKRKGKAAQDEI. scapularis (79) KITLTGVEGKDLELRGKDQEAPVESGKRSAWHRQQEDAKEDRRTQVVEDEConsensus (101) DET E E EEK A R E K A DE151 200M. musculus (108) YEE-DSSDEEDIRNTVGNVPLAWYDEFPHVGYDLDGKRIYKPLRTRDELDD. melanogaster (148) YQDSDTSDEEDIRNTVGNIPMHWYDEYKHIGYDWDAKKIIKPPQG-DQIDH. sapiens (109) YAE-DSSDEEDIRNTVGNVPLEWYDDFPHVGYDLDGRRIYKPLRTRDELDA. gambiae (127) YAAGDTSDEEDIRNTVGNIPMHWYDEYKHVGYDWDAKKIIKAKKG-DAIDI. scapularis (129) YAF-DSSDEEDVRNTVGNIPLEWYEHYPHIGYDLEGKPILKPPRV-SDLDConsensus (151) YAE DSSDEEDIRNTVGNIPL WYDEYPHVGYDLDGKKIIKP R DELD201 250M. musculus (157) QFLDKMDDPDFWRTVQDKMTGRDLRLTDEQVALVHRLQRGQFGDSGFNPYD. melanogaster (197) EFLRKIEDPDFWRTVKDPLTGQDVRLTDEDIALIKRIVSGRIPNKDHEEYH. sapiens (158) QFLDKMDDPDYWRTVQDPMTGRDLRLTDEQVALVRRLQSGQFGDVGFNPYA. gambiae (176) DFLQRMEDPNFWRTVTDPQTGQKVVLSDEDIGLIKRIMSGRNPDAEYDDYI. scapularis (177) DFLRKMDDPNYWRTVKDKSTGQDVVLTDEDVDLIQRLQKGQFPSSTTDPYConsensus (201) DFL KMDDPDFWRTV DPMTGQDVRLTDEDVALIKRLQSGQFPDS FDPY251 300M. musculus (207) EPAVDFFSGDIMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIKPD. melanogaster (247) EPWIEWFTSEVEKMPIKNVPDHKRSFLPSVSEKKRVSRMVHALKMGWMKTH. sapiens (208) EPAVDFFSGDVMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIQPA. gambiae (226) EPFIEWFTSEVEKMPIRNIPESKRSFLPSKAEKHKIGRYVHALKMGWMKTI. scapularis (227) EPFEDIFSHETMIHPVTRHPPQKRSFVPSRIEKAMVSKMVHAIKMGWIKPConsensus (251) EPFIDFFS EVMIHPVTN P KRSFIPSLVEK KVSRMVHAIKMGWIKP301 350M. musculus (257) RRPHD------PTPSFYDLWAQEDPNAVLG-RHKMHVPAPKLALPGHAESD. melanogaster (297) TEEVEREKQAKRGPKFYMLWETDTSREHMR-RIHDPVSAPKRDLPGHAESH. sapiens (258) RRPRD------PTPSFYDLWAQEDPNAVLG-RHKMHVPAPKLALPGHAESA. gambiae (276) MAEKRRLEAIRRQPKFYMLWTTDHGKEEMR-RIHDHVAAPKRMLPGHAESI. scapularis (277) RVKKH------DPERFSLLWDKDDSTAGSNERMQRHIPAPKMKLPGHEESConsensus (301) R KD PKFYMLW DD A L RI HVPAPKL LPGHAES351 400M. musculus (300) YNPPPEYLPTEEERSAW--MQQEPVERKLNFLPQKFPSLRTVPAYSRFIQD. melanogaster (346) YNPPPEYLFDAKETKEWLKLKDEPHKRKLHFMPQKFKSLREVPAYSRYLRH. sapiens (301) YNPPPEYLLSEEERLAW--EQQEPGERKLSFLPRKFPSLRAVPAYGRFIQ52
Gene Therapy and Molecular Biology Vol 7, page 53A. gambiae (325) YNPPPEYLFDEKELEEWNKLANQPWKRKRAYVPQKYNSLREVPGYTRYVKI. scapularis (321) YNPPAEYLFTEEEEAKWR--EQEPEERRINFLPAKYPCLRAVPAYERFIEConsensus (351) YNPPPEYLFTEEE W L QEP ERKL FLPQKFPSLR VPAYSRFI401 450M. musculus (348) ERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPVCQALVYRGD. melanogaster (396) ERFLRCLDLYLCPRAKRVKLNIDAEYLIPKLPSPRDLQPFPTVESMVYRGH. sapiens (349) ERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPTCQALVYRGA. gambiae (375) ERFLRCLDLYLAPRMRRSRVAVGAEYLIPKLPSPRDLQPFPTLQNLIYTGI. scapularis (369) ERFERCLDLYLCPRQRKMRVNVDAEDLIPQLPKPKDLQPFPSIQSIVYEGConsensus (401) ERFERCLDLYLCPRQRKMRVNVDAEDLIPKLPRPRDLQPFPTIQALVYRG451 500M. musculus (398) HSDLVRCLSVSPGGQWLASGSDDGTLKLWEVATARCMKTVHVGGVVRSIAD. melanogaster (446) HTDLVRSVSVEPKGEYLVSGSDDKTVKIWEIATGRCIRTIETDEVVRCVAH. sapiens (399) HSDLVRCLSVSPGGQWLVSGSDDGSLRLWEVATARCVRTVPVGGVVKSVAA. gambiae (425) HTSLIRCISVEPKGEYIVTGSDDMTVKIWEISTARCIRTIPTGDIVRSVAI. scapularis (419) HTDCVLCLSLEPAGQFFASXSEDGTVRIWELLTGXCLKKFQFEAPVKSVAConsensus (451) HTDLVRCLSVEPGGQWLVSGSDDGTVKIWEIATARCIRTI GGVVRSVA501 550M. musculus (448) WNPNPTICLVAAAMDDAVLLLNPALGDRLLVGSTDQLLEAF----TPPEED. melanogaster (496) WCPNPKLSIIAVATGNRLLLVNPKVGDKVLVKKTDDLLAEAPSQDVIESEH. sapiens (449) WNPSPAVCLVAAAVEDSVLLLNPALGDRLVAGSTDQLLSAF----VPPEEA. gambiae (475) WCPNSKISLVAAASGKRVLLINPKVGDYMLVKKTDDLLTEAPRSDTVDSEI. scapularis (469) WCP--VVVPMKLCVDKTVSMLDAGVTDKLLPFTTGHRVVCPPRRVLGPGGConsensus (501) WCPNP I LVAAAVD VLLLNPAVGDKLLV STD LL P V P E551 600M. musculus (494) PALQPARWLEVSEEEHQRGLRLRICHSKPVTQVTWHGRGDYLAVVLSSQED. melanogaster (546) RIKTAVQWSNAEADEQEKGVRVVITHFKPIRQVTWHGRGDYLATVMPEGAH. sapiens (495) PPLQPARWLEASEEERQVGLRLRICHGKPVTQVTWHGRGDYLAVVLATQGA. gambiae (525) RIRSAVQWGEVTEEEKKLGVRIVITHFREVRQVTWHGRGDYFATVMPDGAI. scapularis (517) GSGVGADVGLLSRVPLPGGASAGRSPPR-CGAGDVALEGRLLCHCHGRGTConsensus (551) AA W EVSEEE GLRL ITH KPV QVTWHGRGDYLA VL GA601 650M. musculus (544) HTQVLLHQVSRRRSQSPFRRSHGQVQCVAFHPSRPFLLVASQRSIRIYHLD. melanogaster (596) NRSALIHQLSKRRSQIPFSKSKGLIQFVLFHPVKPCFFVATQHNIRIYDLH. sapiens (545) HTQVLIHQLSRRRSQSPFRRSHGQVQRVAFHPARPFLLVASQRSVRLYHLA. gambiae (575) YRSVMIHQLSKRRSQVPFSKSKGLIQCVLFHPIKPCLFVATQRHIRVYDLI. scapularis (566) GHRACPSVVHAAVRRLPFSKAKGGVSRVLFHPLRPFLLVACQRTVRVYHLConsensus (601) H VLIHQLSKRRSQIPFSKSKG VQ VLFHPIRPFLLVASQRSIRIYHL651 700M. musculus (594) LRQELTKKLMPNCKWVSSMAVHPAGDNIICGSYDSKLVWFDLDLSTKPYKD. melanogaster (646) VKQELVKKLLTNSKWISGMSIHPKGDNLLVSTYDKKMLWFDLDLSTKPYQH. sapiens (595) LRQELTKKLMPNCKWVSSLAVHPAGDNVICGSYDSKLVWFDLDLSTKPYRA. gambiae (625) VKQLMMKKLYPGCKWISSMAIHPKGDNLLIGTYEKRLMWFDLDLSTKPYQI. scapularis (616) LKQELAKRLTSNCKWISCMGRPPPGDNLLIGTYEKRLMWFDLDLSTKPYQConsensus (651) LKQEL KKLMPNCKWISSMAIHP GDNLLIGTYDKKLMWFDLDLSTKPYQ701 750M. musculus (644) VLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVD. melanogaster (696) TMRLHRNAVRSVAFHLRYPLFASGSDDQAVIVSHGMVYNDLLQNPLIVPLH. sapiens (645) MLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVA. gambiae (675) QLRIHNAAIRSVAFHPRYPLFASAGDDRSVIVSHGMVYNDLLQNPLIVPLI. scapularis (666) QLRIHNAAIRSVAFHPRYPLFASAGDDRSVIVSHGMVYNDLLQNPLIVPLConsensus (701) LRIHK AIRSVAFHPRYPLFASGSDD SVIVSHGMVYNDLLQNPLIVPL751 790M. musculus (694) KVLKGHTLTRDLGVLDVAFHPTQPWVFSSGADGTIRLFS-D. melanogaster (746) KKLQTHEKRDEFGVLDVNWHPVQPWVFSTGADSTIRLYT-H. sapiens (695) KVLKGHVLTRDLGVLDVIFHPTQPWVFSSGADGTVRLFT-A. gambiae (725) RRLKNHAVVNDFSVFDVVFHPTQPWVFSSGADNTVRLYT-I. scapularis (716) RRLKNHAISKGMGVLDCAFHPHQPWIVTAGADSTLRLFT-Consensus (751) KRLK H LTRDLGVLDV FHPTQPWVFSSGAD TIRLFTFigure 4. Analysis of clone 4F1 identical to block of proliferation (Bop1). (A) Alignment of M. musculus (AAH12693), D.melanogaster (NP_611270), A. gambiae (EAA04116), and H. sapiens (AAH07274) protein sequences and the translation product ofclone 4F1 identified as I. scapularis Bop (AY296116). Protein sequences are shown in the single letter amino acid code. Identical aminoacids are shown in red and amino acids conserved in 3-4 of 5 sequences are shown in blue.53
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