Sabato 27 ottobre 2012 - Pacini Editore

COmuNiCaziONi ORali
references
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Classification of Tumours. Pathology and genetics of tumours of
soft tissue and bone. IARC Press: Lyon 2002.
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Division of IAP 2006.
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of an oncofetal antigen (M2Aantigen) expressed on testicular
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specific for seminomas and dysgerminomas. Proc Natl Acad Sci
USA 1986;83:5291-5.
6 Bailey D, Marks A, Stratis M, et al. Immunohistochemical staining of
germ cell tumors and intratubular malignant germ cells of the testis
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antiseminoma antibody. Mod Pathol 1991;4:167-71.
7 Sonne SB, Herlihy AS, Hoei-Hansen CE, et al. Identity of M2A
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developing testis, testicular carcinoma in situ and germ-cell tumours.
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of the testis: case report with electron microscopic
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CIntec® PLUS immunocytochemistry:
a tool for the cytological diagnosis
of glandular lesions of the cervix uteri
A. Ravarino1 , S. Nemolato1 , E. Macciocu2 , M. Fraschini3 ,
G. Senes1 , G. Faa1 , G. Negri2 1 Department of Pathology, University Hospital San Giovanni di Dio of
Cagliari, Italy, 2 Department of Pathology, Central Hospital Bolzano, Bolzano,
Italy; 3 Department of Electrical and Electronic Engineering, Univeristy
of Cagliari, Italy
Cytology of glandular lesions of the cervix uteri is sometimes difficult
to evaluate because reactive conditions of the endocervical
epithelia may mimic neoplasia. CINtec PLUS is a novel biomarker
that combines p16 and Ki67 using a double stain technique.
In squamous lesions of the cervix uteri, CINtec Plus has already
shown a high sensitivity and specificity 1 . As far as we know,
however, no study evaluated CINtec PLUS on a large group of
histology-confirmed glandular lesions of the cervix uteri.
In this study, we evaluated the usefulness of CINtec PLUS p16/
Ki-67 double stain for the diagnosis of glandular lesions of the
cervix uteri in liquid-based cytologic specimens 2 .
The study included 47 abnormal ThinPrep (Hologic) liquid-based
cytologies with a subsequent histological diagnosis of adenocarcinoma,
in situ or with early invasion, and 16 samples with negative
follow-up. All samples were stained with CINtec PLUS p16/
Ki-67 double stain (Roche-mtm, Heidelberg).
Of the neoplastic samples, 7 were excluded because of insuf-
Tab. I. CiNtec pluS immunocytochemistry in 63 liquid-based cytologies.
323
ficient residual cellularity or loss of neoplastic cells. Of the adequate
samples, 92.5% were stained with Cintec PLUS (Fig. 1),
whereas 7.5% were judged inconclusive. The samples of all inconclusive
cases had been stored at room temperature for at least
3 years. Of the 16 negative samples, 15 (93.8%) stained negative
and only one (6.2%) showed positive clusters of cells (Tab. I).
By applying the Fisher’s exact test sensitivity, specificity, positive
predictive value, and negative predictive value were 93.75%,
97.37%, 83.33% and 92.50% respectively.
In conclusion, our study shows that CINtec PLUS may be helpful
for the diagnosis of glandular lesions of the cervix uteri and
may reduce the risk of false negatives as well as the number of
uncertain borderline reports.
references
1 Schmidt D, Bergeron C, Denton KJ, et al. European CINtec Cytology
Study Group. p16/Ki-67 Dual-Stain cytology in the triage of ASCUS
and LSIL papanicolaou cytology: results from the european equivocal
or mildly abnormal papanicolaou cytology study. Cancer Cytopathol
2011;119:158-66.
2 Ravarino A, Nemolato S, Macciocu E, et al. Evaluation of CINtec®
PLUS immunocytochemistry as a tool for the cytological diagnosis of
glandular lesions of the cervix uteri. Am J Clin Pathol 2012, in press.
Large cell neuroendocrine carcinoma of the ovary
associated with well differentiated neuroendocrine
tumor of the appendix
B.J. Rocca1 , M.R. Ambrosio1 , M.A.G.M. Butorano1 , A. Ginori1 ,
A. Ambrosio2 , C. Cardone1 , M. Vestri1 , R. Santopietro1 1 Department of Human Pathology and Oncology, Pathological Anatomy
Section, University of Siena, Italy; 2 University “Magna Graecia” of Catanzaro,
Italy
Background. neuroendocrine differentiation may be documented
in a variety of ovarian tumors including surface epithelial tumors,
Follow-up CINtec + CINtec - Inconclusive Inadeguate Total
Positive 37 (92.5%) 0 3 (7.5%) 40
Negative 1 (6.2%) 15 (93.8%) 0 16
Excluded 2* 5 7
total 38 17 3 5 63
* no residual neoplastic cells
Fig. 1. aiS. Sheet of positive cells: the red-stained nuclei are readily
evident on the background of the brown-stained cytoplasm (40x).