Sabato 27 ottobre 2012 - Pacini Editore

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Immunoreactions were revealed us-
Fig. 1.
ing a streptavidin-biotin enhanced
immunoperoxidase technique (Super
Sensitive MultiLink, Menarini, Florence,
Italy) in an automated autostainer.
Receiver Operative Curve
(ROC) analysis was adopted for
optimal cut-off values. Evaluation
of the immunohistochemical results,
blinded to all patient data, was performed
independently and in blinded
manner by two investigators (MM
and FN). Statistical analyses: Multiple
Correspondence Analysis (MCA)
was used to identify sub-groups of
BC patients with different prognosis,
while uni-and multivariate Cox
regression analyses were applied to
determine the impact of parameters
identified by MCA on 10-yrs Disease
Free Survival (DFS), together
with clinico-pathological features.
Results. In our series of 133 BC
patients (median follow-up of 107
months) the MCA analysis (Fig. 1)
demonstrates the contrast between
high Ki67/p53+/p-Akt+/PI3K+/
HER2+ (Adverse Biologic Factors,
ABF) and presence of relapse (upper
right quadrant) vs low Ki67/
p53–/p-Akt–/PI3K–/HER2– BC and
no relapse (lower left quadrant).
Therefore, this statistical approach Fig. 2.
may define an Unfavorable Biologic
Profile (UBP) associated to disease
progression considering the presence/absence
of at least 3 variables
out of the 5 selected as discriminative
power, (UBP≥3 ABF) and a
Favourable Biologic Profile (FBP