Message - 7th IAL Symposium

2A-2-P
Lichen: from genome to ecosystem in a changing world
(2A-2-P5) Submission ID: IAL0073-00001
SPECIES OF GENERA PARMELIA IN RUSSIAN FAR EAST
Chabanenko S. I. 1
1 Russian Academy of Science, Sakhalin Botanical Garden, Yuzhno-Sakhalinsk, Russia
Genera Parmelia is presented in the south of Russian Far East by 14 species. Preliminary revision
species in herbariums of the Far East (TIG, BGI, SAKH), and also LE (St.-Petersburg) has revealed a number
of species which require revision. Morphological variability such species differ: P. fertilis, P. adaugescens. Last
we will well distinguish only at presence apothecia since has large disputes with a thick cover. P. fertilis at well
developed and plentiful apothecia always has poorly developed spoors. Thus the size and the form of lobes,
presence adventives lobules, the size and the form pseudocyphellae vary at different samples. Stables morphological
signs differ P. omphalodes, P. praesquarrosa, P. saxatilis. P. squarrosa exists in two morphological forms.
Some samples have well developed marginal and laminal isidia, pseudocephellae form more less developed
network. Other samples differ more extended, linear lobes, with poorly developed laminal pseoducyphellae (they
are only for tops of lobes). Lobes plane to slightly concave. The tendency for isidia to be concentrated along the
lobe margins. Isidia cylindrical to coralloid and longer, than at the form 1. Samples P. laevior also it is presented
by 2 forms. There is a classical form linear lobe to 3 mm in width more often. On an island of Shikotan (Sakhalin
District) the form with shorter and wide, tiled leaning lobes and thick cortex has been met. Apothecia are plentiful
at two forms; the size dispute does not differ. Samples P. sulcata in the Far East with squarrosely rhizines and
with marginal soredia. Laminal soredia thus are absent or are extremely rare. There are samples at whom the
medulla C+ turns yellow, at others C –. P. marmarisa, P. isidioclada, P. pseudolaevior, P. fraudans – rare lichens
on south part of Russian Far East; it is necessary more quantity of samples to analyze these species. Thus the
preliminary data has shown that some species of genera Parmelia from Russian Far East need in analysis using
molecular, morphological and chemical characters.
(2A-2-P6) Submission ID: IAL0129-00003
THE SPECIES OF CETRARIA ACULEATA GROUP (PARMELIACEAE) IN UKRAINE:
IDENTIFICATION PROBLEMS
Nadyeina O. V. 1 , Lutsak T. V. 2 , Grakhov V. P. 3 , Blum O. B. 4
1 Lichenology & Bryology, M. G. Kholodny Institute of Botany, Kyiv, Ukraine
2 Department of Botany, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
3 Center for Collective Use of Equipment Hplc, M. M. Gryshko National Botanical Garden, Kyiv, Ukraine
4 Chemotaxonomy and Bioindication, M. M. Gryshko National Botanical Garden, Kyiv, Ukraine
Over 300 specimens of Cetraria aculeata group (C. aculeata, C. muricata, C. steppae) mainly from
Ukraine and also from Poland, Hungary, Czech Republic, Russia, Azerbaijan, Kyrgyzstan and Turkey were studied
for morphology, anatomy, chemistry, substrate specifity and geographic distribution. Previously known high
level of phenotypic plasticity of these species (Kärnefelt, 1986) is confirmed upon the analyses of the specimens.
Colored spot and microcrystal tests were used for norstictic acid detection in Cetraria specimens, but in this
case they show inapplicability due to interfering components, while TLC and HPLC seem to be effective. HPLC
demonstrates high variance of norstictic acid content in the studied thalli: from trace, minor, medium to major
quantities which are not determined ecologically. The specimens collected from the same populations growing in
the southern Russia and Ukraine regions and also Hungary may contain different amount of norstictic acid, even
traces (detected by TLC as absence). Therefore distinguishing of C. steppae and C. aculeata species based on
the presence/absence of norstictic acid (Kärnefelt, 1986) is considered as foundless. Taxonomic status of these
southern populations (usually identified as C. steppae) should be investigated using specific molecular markers.
Possible evolutionary and adaptive role of norstictic acid is of open question. Correct identification of C. steppae
and C. aculeata species is also significant since C. steppae is included in the Red Data Book of Ukraine as
vulnerable species.
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