Message - 7th IAL Symposium

More documents

Recommendations

Info

Lichen: from genome to ecosystem in a changing world 4I-P (4I-P4) Submission ID: IAL0039-00001 OPTIMIZATION FOR SECONDARY METABOLITES PRODUCTION FROM TROPICAL LICHEN MYCOBIONTS Sanglarpcharoenkit M. 1 , Chokchaisiri R. 2 , Suksamrarn A. 3 , Whalley A. 4 , Sangvichien E. 1 1 Department of Biology, Ramkhamhaeng University, Bangkok, Thailand 2 Department of Chemistry, University of Phayao, Phayao, Thailand 3 Department of Chemistry, Ramkhamhaeng University, Bangkok, Thailand 4 School of Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom Lichens are resources for novel compounds with many of them having importance in medicine and other fields. Intact lichens cannot be cultivated but their mycobionts can be axenically grown in laboratory from ascospore discharge or tissue culture techniques. Frequency of spore discharge, germination and colony development were varied among tropical lichen species. Mycobiont cultures from stock at The Lichen Research Unit, Ramkhamhaeng University (RAMK) were screened for their abilities to produce biological activities. Among these four mycobiont species; Graphis sp., Graphina albissima, Ocellularia punctulata and Pyrenula kurzii were selected regarding their potential to produce metabolites with antimicrobial activities. From 7 different media for stimulation of growth of mycobionts, Malt-Yeast Extract medium was chosen for its superior performance and used for further experimentation. The mycobionts were grown on both solid and liquid media and the secondary metabolites produced under various conditions were examined. Static and shake liquid cultures with various supporting materials were examined for growth of mycobionts, however in these conditions growth rate were higher but the metabolites produced were lower in both number and quantities. Another way to culture the mycobionts was solid medium and was also considered an easier method and mycobionts were grown for period of 27 weeks at room temperature, cells and pieces of agar block were removed at intervals and extracted with methanol. Chemical profiles detected by Thin Layer Chromatography (TLC) indicated that the metabolites produced increased gradually during 9-15 weeks of incubation under these conditions. Comparison of pH between acidic, neutral and alkaline conditions for growth of these mycobionts showed that the optimize pH for all of them was at neutral pH. Some lichen substances absorb ultraviolet light and protect the algae from too intensive irradiation, in order to induce metabolite production in mycobionts cultures, both short and long wavelength UV light were investigated. The results indicated that the number of spots on TLC plates were decreased, however the conditions in this experiment were not entirely suitable. Scale up for high numbers of cell mass and secreted metabolites were done and further studied on the chemical structures of new chemical compounds were investigated and are discussed. (4I-P5) Submission ID: IAL0068-00001 LICHEN DIVERSITY «HOT SPOT» IN KRONOTSKY NATURE RESERVE, KAMCHATKA Stepanchikova I. S. 1 , Himelbrant D. E. 1 1 Botany, Saint-Petersburg State University, Saint-Petersburg, Russia Primeval old-growth spruce forests in vicinity of Levaya Schapina River (Kronotsky Nature Reserve, Eastern Kamchatka) were investigated. The area appeared to be exceptionally rich in lichens, particularly spruce epiphytes: more than 130 lichen species were recorded on bark of Picea ajanensis. The most important finding is a population of Erioderma pedicellatum. The species was rather abundant on twigs of old spruces, where it grew together with Lobaria pulmonaria, L. scrobiculata, Nephroma helveticum, Ramalina thrausta, Sticta limbata and other species. At least thirty lichen species found near Schapina are known as indicators of undisturbed old-growth forests, among them Arthonia zwackii (new for Russian Far East), Usnea longissima (extremely rare in the North of Russian Far East), Pseucyphelaria crocata and Schismatomma pericleum (new for the North of Russian Far East). 140
The 7 th International Association for Lichenology Symposium 2012 (4I-P6) Submission ID: IAL0070-00001 CHEMICAL STUDIES ON TWO LICHENS OF THE GENUS ERIODERMA FROM MALAYSIA Samsudin M. B. 1 , Din L. B. 2 , Hamat A. B. 2 , John E. A. 3 1 Faculty Sciences and Technology, University Kebangsaan Malaysia, Bangi, Selangor, Malaysia 2 Faculty of Science & Technology, UKM, Malaysia, Malaysia 3 Faculty of Science, ANU, Canberra, Australia Chemical studies on two lichens, Erioderma phaeorhizum vainio sens. lat. and Erioderma tomentosum Hue, collected from Brincang, Cameron Highlands Malaysia were carried out. The structure of the compounds isolated from both lichens were elucidated by chromatographic, spectroscopic, microanalysis methods and unambiguous synthesis. Two new depsidones, hypophysciosporin [methyl 2-chloro-3,8-dihydroxy-1,4,6,9tetramethyl-11-oxo-11H-dibenzo[b,e] [1,4] dioxepin-7-carboxylate] and 3-o-methylhypophysciosporin [methyl 2-chloro-8-hydroxy-3-methoxy-1,4,6,9-tetramethyl-11-oxo-11H-dibenzo[b,e] [1,4] dioxepin-7-carboxylate], have been isolated from Erioderma phaeorhizum together with eight known depsidones, vicanicin, isovicanicin, norvicanicin, o-methylvicanicin, eriodermin, argopsin, granulatin and physciosporin. In addition the chroman, vitamin E acetate , has been isolated from Erioderma tomentosum, the first reported occurrence of this compound in a lichen. The other compounds which have been isolated from this lichen were the depsidones, vicanicin , norvicanicin and argopsin, and the depside, atranorin . The minor unknown compounds have been isolated from Erioderma phaeorhizum. The structure of the first compound is related to 3-o-methylhypophysciosporin while the second compound is related to vitamin E. Biosynthesis pathway of these metabolites is proposed. Possibly it involves secondary modification of the simplest β-orcinol depsidone, hypoprotocetraric acid, via several stages of enzymatic reactions involving oxidation, o-methylation of hydroxyl and carboxyl groups, decarboxylation and chlorination. (4I-P7) Submission ID: IAL0088-00001 REDISCOVERY OF A FORGOTTEN LICHEN GENUS WITH UNIQUE CONIDIOMATA FROM NEW ZEALAND Ludwig L. R. 1 , Lücking R. 2 1 Department of Botany, University of Otago, Dunedin, Otago, New Zealand 2 Department of Botany, The Field Museum of Natural History, Chicago, Illinois, United States A peculiar lichen with unique conidiomata from southern New Zealand, Nukaia cupulifera, is described as new to science. The conidiomata closely resemble apothecia of a gyalectoid lichen but in section reveal their conidiomatal nature, owing close resemblance to the campylidia of the family Pilocarpaceae. The new taxon is closely related to and congeneric with a lichen described in 1955 as Ephelidium heardense C.W. Dodge & E.D. Rudolph, but differs in certain anatomical and chemical details. Unfortunately, the generic name Ephelidium C.W. Dodge & E.D. Rudolph cannot be used, although it was accepted in Species Fungorum and the Checklist Of The Lichens Of Australia And Its Island Territories, because it is a later homonym of the unrelated, nonlichenized, phytopathogenic ascomycete genus Ephelidium C. Spegazzini from 1921. We therefore introduce the replacement name Nukaia for this peculiar taxon. A structure apparently similar to the conidiomata of Nukaia was described in 1890 by Müller Argoviensis as “orthidium”; unfortunately, the specific material was not cited and was not found in the G herbarium, so we are unable to evaluate whether a similar or congeneric lichen is involved. It seems, however, unlikely, since Müller Argoviensis described this structure from a foliicolous lichen growing in the Atlantic rain forest of Brazil. The generic position of Nukaia cupulifera was tested using ITS sequence analysis, which places it in Ramalinaceae but not within any of the known genera of that family. 141 4I-P
Page 1 and 2:
The 7 th IAL Symposium 2012 Lichens
Page 3 and 4:
The 7 th International Association
Page 5 and 6:
Welcome Message The 7 th Internatio
Page 7 and 8:
Welcome Message Dear IAL7 Participa
Page 9 and 10:
Report The 7 th International Assoc
Page 11 and 12:
Opening Address The 7 th Internatio
Page 13 and 14:
IAL COUNCIL (2008-2012): The 7 th I
Page 15 and 16:
Page 17 and 18:
SYMPOSIUM VENUE The 7 th Internatio
Page 19 and 20:
FLOOR PLAN The 7 th International A
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Scientific Program P14
Page 37 and 38:
s s The 7 th International Associat
Page 39 and 40:
Page 41 and 42:
s s s s s s The 7 th International
Page 43 and 44:
s s The 7 th International Associat
Page 45 and 46:
s s s s s The 7 th International As
Page 47 and 48:
s s s The 7 th International Associ
Page 49 and 50:
s s s s The 7 th International Asso
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
s s s s s s s The 7 th Internationa
Page 63 and 64:
Page 65 and 66:
Page 67:
ABSTRACTS OF CONTRIBUTED PAPERS (OR
Page 70 and 71:
1 2 3
Page 72 and 73:
Lichen: from genome to ecosystem in
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
1A-O Lichen: from genome to ecosyst
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
1B-O Lichen: from genome to ecosyst
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
2A-1-O Lichen: from genome to ecosy
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
3B-1-O Lichen: from genome to ecosy
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161: Lichen: from genome to ecosystem in
Page 170 and 171: 2A-1-P Lichen: from genome to ecosy
Page 236 and 237: 3B-1-P Lichen: from genome to ecosy
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276:
1 2 3
show all

Message - 7th IAL Symposium

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?