Message - 7th IAL Symposium

Lichen: from genome to ecosystem in a changing world
4I-P
4I: Lichenological research in South-East Asia and the Pacific region
(4I-P1) Submission ID: IAL0026-00001
THE LICHENS FAMILY PHYSCIACEAE (LECANORALES, ASCOMYCOTA)
IN THAILAND
Meesim S. 1 , Mongkolsuk P. 1 , Boonpragob K. 1 , Buaruang K. 1 , Manoch L. 2 , Kalb K. J. 3
1 Biology, Ramkhamhaeng University, Bangkok, Thailand
2 Plant Pathology, Kasetsart University, Bangkok, Thailand
3 Botany, University of Regensburg, Regensburg, Germany
Members of the lichens family Physciaceae were collected from sixteen provinces and eleven forest
types in Thailand and taxonomically identified in the period between 1995 and 2010. They were classified under
the rubrics of nineteen genera and one hundred twenty-eight species. Nine taxa of foliose and twenty-six taxa of
crustose lichens were newly described in Thailand. In addition, nine taxa of foliose and twenty-seven of crustose
lichens are expected to be identified as new species previously unknown to science.
(4I-P2) Submission ID: IAL0029-00001
IN VITRO ANTIPROLIFERATIVE ACTIVITY OF LAURERA SPECIES (TRYPETHELIACEAE,
PYRENULALES) CRUDE EXTRACTS AGAINST FOUR HUMAN CANCER CELL LINES
Vongshewarat K. 1 , Rodtong S. 2 , Siripong P. 3
1 Lichen Research Unit, Lichen Research Unit, Thailand, Bangkok, Thailand
2 Microbiology, Nakhon Ratchasima, Thailand
3 Research Division, National Cancer Institute, Bangkok, Thailand
Laurera species is a common genera of crustose lichens in Thailand, which has been reported to
produce antifungal compounds and photoprotective secondary products. The chemical constituents of several
lichens have been shown to have biological and pharmaceutical activity including antibacterial, antifungal, antiproliferative
and cytotoxic activity. The aim of this study is to investigate in vitro antiproliferative activity of Laurera
species crude extracts with different solvents of increasing polarity. Four crustose lichen species, Laurera benguelensis,
L. madreporiformis, L. meristospora, and L. subdiscreta, were extracted successively with chloroform
and methanol using maceration process. The cytotoxic activity of the eight lichen extracts was evaluated in vitro
using four human cancer cells: KB (human epidermoid carcinoma), HepG2 (human hepatocellular carcinoma)
HeLa (human cervical carcinoma) and MCF-7 (human breast carcinoma) and a non-cancerous cells, Vero (African
green monkey kidney cells). The inhibition of cell proliferation by crude extracts was determined by MTT
colorimetric assay and g/ml. The crude chloroform extract of the four µ30 < active standard value at IC 50 crustose
lichens showed against four human cancer cells (KB, HepG2, HeLa and g/ml, µMCF-7) at the IC 50 values
of 0.3-11.0, 0.49-24.0, 0.59-19.0 and 0.9-35.5 respectively, whereas the methanolic extracts had weak activity
(IC 50 vales of g/ml) but exhibit low toxicity with normal cell than other crude µ14.5-100 g/ml). Therefore, the nonpolar
lichen compounds µ extracts IC 50 vales of 45-59 showed higher antiproliferative and cytotoxic activity than
polar lichen compounds. Purification and identification of the bioactive components from these active lichens are
under investigation.
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